| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-249SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF800-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34531-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44415 | |||
| Azide and BSA Free | NBP2-34743-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47814-0.1mg | |||
| IHC-Prediluted | NBP2-48304 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33164 | |||
| IHC-Prediluted | NBP2-44413 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44417 | |||
| Azide and BSA Free | NBP2-34571-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44417 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08777 | |||
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Beclin-1-Antibody---BSA-Free-Western-Blot-NB500-249-img0018.jpg "Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody - BSA Free [NB500-249] - Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Beclin-1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-249-img0013.jpg)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB500-249] - Detection of Beclin 1 (red) in Pheochromocytes of the Adrenal Medulla 40x.](http://images.novusbio.com/fullsize/Beclin-1-Antibody---BSA-Free-Immunohistochemistry-NB500-249-img0020.jpg)
![Flow Cytometry: Beclin 1 Antibody - BSA Free [NB500-249] - An intracellular stain was performed on U-87MG cells with Beclin 1 Antibody NB500-249 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Beclin-1-Antibody---BSA-Free-Flow-Cytometry-NB500-249-img0024.jpg)
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Representative Western blot images and quantitative analyses of Beclin 1 from the left hemisphere of rat brains at different time points after SAH. Sample size is 36, n = 6 per group. Data were presented as mean +/- SD. F = 12.37 for Beclin 1. *P 0.05 versus TBI + vehicle group. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241537194.jpg)
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - AMPK knockout interrupts & decreases apoptosis in cochlea. (C) WB results show changes in autophagy-related proteins in cochleae of aging mice. There is a remarkable decline of mTOR signaling (Tg-B1 vs. AMPK+/−/Tg-B1, p 0.05 versus TBI + DMSO group. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416161847.jpg)
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Inhibition of lysosomes leads to G1 arrest by inactivating cyclin E/CDK2 complex. (A) CQ treatment (10, 50, or 100 μM, 24 h) leads to cell cycle G1 arrest. Quantitation of different cell cycle stages in TM3 cells in the presence or absence of CQ. (B–D) EdU incorporation & mitotic index are reduced in CQ-treated TM3 cells. (B) Immunostaining of EdU (red) & DAPI (blue) in scramble control (CTL) or CQ treated TM3 cells. Quantitation of EdU incorporation (C) or mitotic index (D) in scramble control (CTL) or CQ-treated TM3 cells. These results are mean +/− SD from three independent experiments; more than 1000 cells were counted in each individual group. (E–G) CQ inhibited cyclin E1 expression & CDK2 activation. (E,F) Whole cell extracts of CQ-treated TM3 cells at the concentration of 10, 50, or 100 μM are analyzed by immunoblot with antibodies against Beclin1, cyclin D, cyclin A, cyclin E1, CDK2, phosphorylated CDK2 at Thr160 (pCDK2) & alpha -tubulin. (G) Quantitation of relative intensity of cyclin E & pCDK2 in (F). *P 50 cells were counted under a dissecting microscope. Image collected & cropped by CiteAb from the following publication (//jhoonline.biomedcentral.com/articles/10.1186/1756-8722-7-23), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241618261.jpg)
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - Fucoxanthin activated autophagy after TBI.(A) Representative images of immunofluorescence for LC3 surrounding the injured cortex. LC3 punctate dots were observed in the cytoplasm by immunofluorescent staining of LC3 (red). Neuron cells & nuclei are labeled with NeuN (green) & DAPI (blue), respectively. Magnification: 40 x. Scale bar: 50 mm. (B) Mice brain tissues were collected 1 day after TBI in different groups, & the expression of LC3, Beclin-1 & p62 was measured by western blot. Fucoxanthin treatment significantly increased the level of LC3-II & Beclin-1 while decreasing the level of p62 after TBI. (C) 3-MA (400 nM) was injected i.c.v. 30 min before TBI. Mice were then subjected to TBI & treatment of fucoxanthin 30 min after TBI. Pretreatment with 3-MA significantly attenuated fucoxanthin-induced activation of autophagy & suppression of apoptosis & oxidative stress in the ipsilateral cortex. Data are presented as mean ± SEM, n = 6 per group; **p < 0.01, ***p < 0.001 versus sham group; #p < 0.05, ##p < 0.01 versus TBI + vehicle group; &&p < 0.01, &&&p < 0.001 versus TBI + fucoxanthin group. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28429775), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416171472.jpg)
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - GABARAP interacts with AC3 via LIRs of AC3 in a ciliary expression‐dependent manner. A‐E) WB (A) & densitometric quantification of the expression of GABARAP (B), ATG5 (C), VPS34 (D), & Beclin1(E) in the hypothalami of AC3+/+, AC3−/−, & hAC3 mice (n = 3 mice per group). Actin served as the loading control. F) Representative IF co‐staining with AC3 & GABARAP antibodies in the VMHs of WT mice. F′) A higher magnification of the boxed region. Scale bars: F) 20 µm; F′) 5 µm. G) Representative images showing the expression levels of GABARAP & AC3 in the VMHs of VMH pIFT88‐AC3 KD mice & the controls. Scale bars: 20 µm. H) Schematic representation of GABARAP binding LIRs at aa488‐aa493 & aa958‐aa963 of AC3. I) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (LIR1 Mut), or AC3 (LIR2 Mut). LgG served as the negative control. J) Pull‐down analysis of GABARAP & AC3. K) Co‐IP analysis of GABARAP & AC3 (WT), AC3 (296 Mut), or AC3 (465 Mut). LgG served as the negative control. L) Schematic representation of AC3 regulating GABARAP. Data represent the mean ± SEM; *p < 0.05 & **p < 0.01; one‐way ANOVA & Bonferroni pairwise comparisons. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34783461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-249_rabbit-polyclonal-beclin-1-antibody-310202416165618.jpg)
![Western Blot: Beclin 1 Antibody - BSA Free [NB500-249] - EMPA treatment enhanced autophagy in failing hearts. (A) Transmission electron microscopy (TEM) showed autophagosomes in the hearts. Red arrows point to autophagosomes. (B) EMPA activated autophagy pathway by inhibiting mTOR pathway. (C) RT-PCR results showed that the mRNA expression of Beclin1, Atg7 & LC3 were increased by EMPA treatment after TAC. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; AMPK, AMP-activated protein kinase; mTOR, mammalian target of rapamycin; Ulk, Unc-51 like autophagy activating kinase; Atg7, autophagy related 7; LC3, light chain 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-249_rabbit-polyclonal-beclin-1-antibody-31020241616566.jpg)
![Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Rat and mouse neonatal cardiomyocytes. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-2H12-Western-Blot-NBP2-32917-img0004.jpg "Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Rat and mouse neonatal cardiomyocytes. Western blot image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) [NBP2-32917] - FFPE human Hodgkin's lymphoma stained with bcl-x Ab (2H12). Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-2H12-Immunohistochemistry-Paraffin-NBP2-32917-img0001.jpg)
![Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Western blot analysis of Jurkat Cell lysate using bcl-X Ab (2H12).](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-2H12-Western-Blot-NBP2-32917-img0003.jpg)
![Immunohistochemistry-Paraffin: bcl-x Antibody (SPM165) [NBP2-32810] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with bcl-x Mouse Monoclonal antibody (SPM165).](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-SPM165-Immunohistochemistry-Paraffin-NBP2-32810-img0001.jpg "Immunohistochemistry-Paraffin: bcl-x Antibody (SPM165) [NBP2-32810] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with bcl-x Mouse Monoclonal antibody (SPM165).")
![Immunohistochemistry-Paraffin: bcl-x Antibody (SPM337) [NBP2-44416] - Human Hodgkin's Lymphoma stained with bcl-x Monoclonal Antibody (SPM337).](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-SPM337-Immunohistochemistry-Paraffin-NBP2-44416-img0001.jpg "Immunohistochemistry-Paraffin: bcl-x Antibody (SPM337) [NBP2-44416] - Human Hodgkin's Lymphoma stained with bcl-x Monoclonal Antibody (SPM337).")
![Western Blot: bcl-x Antibody (BX006) [NBP2-25240] - Analysis of Bcl-X in Jurkat lysate using Bcl-xl antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-BX006-Western-Blot-NBP2-25240-img0008.jpg "Western Blot: bcl-x Antibody (BX006) [NBP2-25240] - Analysis of Bcl-X in Jurkat lysate using Bcl-xl antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: bcl-x Antibody (BX006) [NBP2-25240] - Formalin-paraffin Hodgkin's lymphoma stained with Bcl-xl Ab (BX006). Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-BX006-Immunohistochemistry-Paraffin-NBP2-25240-img0009.jpg)
![Western Blot: bcl-x Antibody (BX006 + 2H12) [NBP2-34290] - Western blot analysis of Jurkat Cell lysate using bcl-X MAb (BX006 + 2H12).](http://images.novusbio.com/fullsize/bcl-x-Antibody-BX006-+-2H12-Western-Blot-NBP2-34290-img0003.jpg "Western Blot: bcl-x Antibody (BX006 + 2H12) [NBP2-34290] - Western blot analysis of Jurkat Cell lysate using bcl-X MAb (BX006 + 2H12).")
![Western Blot: bcl-x Antibody (BX006 + 2H12) - Azide and BSA Free [NBP2-34571] - Western blot analysis of Jurkat Cell lysate using bcl-X monoclonal antibody (BX006 + 2H12).](http://images.novusbio.com/fullsize/bcl-x-Antibody-BX006-+-2H12-Azide-and-BSA-Free-Western-Blot-NBP2-34571-img0001.jpg "Western Blot: bcl-x Antibody (BX006 + 2H12) - Azide and BSA Free [NBP2-34571] - Western blot analysis of Jurkat Cell lysate using bcl-X monoclonal antibody (BX006 + 2H12).")
![Immunohistochemistry: bcl-x Antibody (5E9) [NBP3-26480] - Image of bcl-x Antibody (5E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26480_rabbit-bcl-x-mab-5e9-2722024845329.jpg "Immunohistochemistry: bcl-x Antibody (5E9) [NBP3-26480] - Image of bcl-x Antibody (5E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: bcl-x Antibody (5E9) [NBP3-26480] - Positive Western Blot detected in: K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: bcl-x Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27, 19, 26 kDa Observed band size: 30 kDa](http://images.novusbio.com/fullsize/nbp3-26480_rabbit-bcl-x-mab-5e9-28220241144266.jpg)
![Western Blot: bcl-x Antibody (2H12) [NBP3-11881] - Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha's anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/bcl-x-Antibody-2H12-Western-Blot-NBP3-11881-img0001.jpg "Western Blot: bcl-x Antibody (2H12) [NBP3-11881] - Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha's anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.")
![Immunohistochemistry-Paraffin: bcl-x Antibody (BCL2L1/2406) [NBP3-07455] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with bcl-x Mouse Monoclonal Antibody (BCL2L1/2406).](http://images.novusbio.com/fullsize/bcl-x-Antibody-BCL2L1-2406-Immunohistochemistry-Paraffin-NBP3-07455-img0002.jpg "Immunohistochemistry-Paraffin: bcl-x Antibody (BCL2L1/2406) [NBP3-07455] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with bcl-x Mouse Monoclonal Antibody (BCL2L1/2406).")
![Immunohistochemistry-Paraffin: bcl-x Antibody (BCL2L1/2406) [NBP3-07455] - Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with bcl-x Mouse Monoclonal Antibody (BCL2L1/2406).](http://images.novusbio.com/fullsize/bcl-x-Antibody-BCL2L1-2406-Immunohistochemistry-Paraffin-NBP3-07455-img0001.jpg)
![ELISA: Human bcl-x ELISA Kit (Chemiluminescence) [NBP2-69949] - Samples were spiked with high concentrations of Human bcl-x and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69949_human-bcl-x-elisa-kit-chemiluminescence-13220241692253.jpg "ELISA: Human bcl-x ELISA Kit (Chemiluminescence) [NBP2-69949] - Samples were spiked with high concentrations of Human bcl-x and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human bcl-x ELISA Kit (Chemiluminescence) [NBP2-69949] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69949_human-bcl-x-elisa-kit-chemiluminescence-4320248595186.jpg)