| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37397-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10168-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-15339SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70151 | |||
![Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Western-Blot-NBP2-37397-img0004.jpg "Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.")
![Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Immunocytochemistry-Immunofluorescence-NBP2-37397-img0005.jpg)
![Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Immunohistochemistry-NBP2-37397-img0008.jpg)
![Flow Cytometry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Flow cytometric analysis of HeLa cells using ALDH2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Flow-Cytometry-NBP2-37397-img0006.jpg)
![Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mAb against human ALDH2 recombinant protein. (Expected MW is 47.4 kDa)](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Western-Blot-NBP2-37397-img0002.jpg)
![Western Blot: ALDH2 Antibody (4G6A3) [NBP2-37397] - Western blot analysis using ALDH2 mAb against HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Western-Blot-NBP2-37397-img0003.jpg)
![Immunohistochemistry: ALDH2 Antibody (4G6A3) [NBP2-37397] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-Immunohistochemistry-NBP2-37397-img0007.jpg)
![ELISA: ALDH2 Antibody (4G6A3) [NBP2-37397] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ALDH2-Antibody-4G6A3-ELISA-NBP2-37397-img0001.jpg)
![Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Western-Blot-NBP1-31749-img0004.jpg "Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ALDH2 Antibody [NBP1-31749] - Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: ALDH2 protein stained by ALDH2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31749-img0002.jpg)
![Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Western-Blot-NBP1-31749-img0003.jpg)
![Western Blot: ALDH2 Antibody [NBP1-31749] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP1-31749 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31749_rabbit-polyclonal-aldh2-antibody-9102024205661.jpg)
![Immunohistochemistry: ALDH2 Antibody [NBP1-31749] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody [N1C1] (NBP1-31749) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31749_rabbit-polyclonal-aldh2-antibody-91020242021938.jpg)
![Immunohistochemistry: ALDH2 Antibody [NBP1-31749] - ALDH2 antibody [N1C1] detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. ALDH2 antibody [N1C1] (NBP1-31749) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp1-31749_rabbit-polyclonal-aldh2-antibody-91020242014645.jpg)
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ALDH1A3 (NBP2-15339) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Western-Blot-NBP2-15339-img0021.jpg "Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ALDH1A3 (NBP2-15339) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ALDH1A3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-15339-img0022.jpg)
![Immunohistochemistry: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jdr/2017/8503754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-NBP2-15339-img0020.jpg)
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - IL25 Deficiency Induced loss of CRC stemness. The expression levels of CSC markers, namely, DCLK1, ALDH1 (NBP2-15339), and CD44, were examined in tumor tissues by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35359953/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH1A3 Antibody - BSA Free-Western Blot-NBP2-15339-img0023.jpg)
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Western-Blot-NBP2-15339-img0010.jpg)
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Western-Blot-NBP2-15339-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-15339-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced with human Aldh1a3 and probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-15339-img0019.jpg)
![Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP2-15339-img0007.jpg)
![Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP2-15339-img0015.jpg)
![Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP2-15339-img0016.jpg)
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Palbociclib induces a potential transition of PN GSCs to MES GSCs(A) qPCR analysis of markers of PN & MES subtypes in G44 & G559 treated with the indicated doses of palbociclib for 5 days. n = 3. Differences were analyzed between the control & the palbociclib-treated groups. NS: not statistically significant; #p < 0.05; *p < 0.01; **p < 0.001. (B) qPCR & immunoblot analysis of ALDH1A3 in three independent PN GSC lines that were treated with 10 nM of palbociclib for 5 days. n = 3. Differences were analyzed between the control & the palbociclib-treated groups. NS: not statistically significant; #p < 0.05; *p < 0.01; **p < 0.001. (C) Cell proliferation analysis of three independent PN GSC lines that were treated with palbociclib (10 nM), DEAB (25 μM), or both for 5 days. The cells were cultured on a laminin (10 μg/ml in poly ornithine)-coated 96-well plate. Cell number was determined by CyQUANT Direct Cell Proliferation assay. n = 3. *p < 0.05, comparison between palbociclib or DEAB treatment with the vehicle control; #p < 0.05, comparison between combination group with palbociclib treatment. G44, CDI = 0.79; G559, CDI = 0.565; G816, CDI = 0.677. Shown are representative data of three independent experiments with similar results. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.19429), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15339_rabbit-polyclonal-aldh1a3-antibody-310202416114717.jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. (a) Immunofluorescent analysis of islets from weight-matched WD-fed mice (top row) & db/db mice (bottom row) showing Nkx6.1 (red), insulin (green), & DAPI (blue). In the merged image, note the loss of double-positive nuclei (DAPI plus Nkx6.1; purple color) in the db/db mice but not in the WD-fed mice. (b) Expression of the Aldh1a3 gene in islets isolated from mice fed a WD for 4 or 12 weeks normalized to mice fed a control diet compared with db/db mice at 8 weeks of age (normalized to lean db/+ controls). ∗∗∗p < 0.001 versus both WD groups by one-way ANOVA. (c) Staining for Aldh1a3 protein (red) & insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29038790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15339_rabbit-polyclonal-aldh1a3-antibody-31020241613622.jpg)
![Western Blot: ALDH2 Antibody (1E5) [H00000217-M01] - Analysis of ALDH2 expression in transfected 293T cell line by ALDH2 monoclonal antibody (M01), clone 1E5.Lane 1: ALDH2 transfected lysate(56.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ALDH2-Antibody-1E5-Western-Blot-H00000217-M01-img0005.jpg "Western Blot: ALDH2 Antibody (1E5) [H00000217-M01] - Analysis of ALDH2 expression in transfected 293T cell line by ALDH2 monoclonal antibody (M01), clone 1E5.Lane 1: ALDH2 transfected lysate(56.4 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: ALDH2 Antibody (1E5) [H00000217-M01] - ALDH2 monoclonal antibody (M01), clone 1E5 Analysis of ALDH2 expression in MCF-7.](http://images.novusbio.com/fullsize/ALDH2-Antibody-1E5-Western-Blot-H00000217-M01-img0004.jpg)
![ELISA: ALDH2 Antibody (1E5) [H00000217-M01] - Detection limit for recombinant GST tagged ALDH2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ALDH2-Antibody-1E5-ELISA-H00000217-M01-img0003.jpg)
![Western Blot: ALDH2 Antibody [NBP2-14280] - Analysis in human liver tissue.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Western-Blot-NBP2-14280-img0003.jpg "Western Blot: ALDH2 Antibody [NBP2-14280] - Analysis in human liver tissue.")
![Immunohistochemistry-Paraffin: ALDH2 Antibody [NBP2-14280] - Staining of human heart shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Immunohistochemistry-Paraffin-NBP2-14280-img0006.jpg)
![Immunohistochemistry-Paraffin: ALDH2 Antibody [NBP2-14280] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Immunohistochemistry-Paraffin-NBP2-14280-img0004.jpg)
![Immunohistochemistry-Paraffin: ALDH2 Antibody [NBP2-14280] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Immunohistochemistry-Paraffin-NBP2-14280-img0005.jpg)
![Immunohistochemistry-Paraffin: ALDH2 Antibody [NBP2-14280] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Immunohistochemistry-Paraffin-NBP2-14280-img0007.jpg)
![Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-27820241514176.jpg "Western Blot: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Lane 5: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-298202413585574.jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (E4-D10-R) [NBP3-32021] - Immunocytochemistry analysis of HeLa cells labeling ALDH2 with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (NBP3-32021) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32021_mouse-aldh2-mab-e4-d10-r-29202410234220.jpg)
![Western Blot: ALDH2 Antibody (7-D8-R) [NBP3-32022] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32022) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: THP-1 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Rat liver tissue lysate (40 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32022_mouse-aldh2-mab-7-d8-r-27820241514177.jpg "Western Blot: ALDH2 Antibody (7-D8-R) [NBP3-32022] - Western blot analysis of ALDH2 on different lysates with Mouse anti-ALDH2 antibody (NBP3-32022) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: THP-1 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Rat liver tissue lysate (40 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ALDH2 Antibody (7-D8-R) [NBP3-32022] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ALDH2 antibody (NBP3-32022) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32022) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32022_mouse-aldh2-mab-7-d8-r-29820241420978.jpg)
![Immunocytochemistry/ Immunofluorescence: ALDH2 Antibody (7-D8-R) [NBP3-32022] - Immunocytochemistry analysis of HepG2 cells labeling ALDH2 with Mouse anti-ALDH2 antibody (NBP3-32022) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH2 antibody (NBP3-32022) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32022_mouse-aldh2-mab-7-d8-r-2920241114821.jpg)
![Western Blot: ALDH2 Antibody [NBP1-52051] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ALDH2-Antibody-Western-Blot-NBP1-52051-img0001.jpg "Western Blot: ALDH2 Antibody [NBP1-52051] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4H2) [NBP2-02102] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4H2-Immunocytochemistry-Immunofluorescence-NBP2-02102-img0003.jpg "Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4H2) [NBP2-02102] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH2.")
![Flow Cytometry: ALDH2 Antibody (4H2) [NBP2-02102] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4H2-Flow-Cytometry-NBP2-02102-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (4H2) [NBP2-02102] - Immunofluorescent staining of HepG2 cells using anti-ALDH2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ALDH2-Antibody-4H2-Immunocytochemistry-Immunofluorescence-NBP2-02102-img0002.jpg)
![Western Blot: ALDH2 Antibody (3F10) [NBP3-15140] - Positive WB detected in: Ntera-2 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver cell lysate. All lanes: ALDH2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 51 KDa. Observed band size: 57 KDa.](http://images.novusbio.com/fullsize/ALDH2-Antibody-3F10-Western-Blot-NBP3-15140-img0002.jpg "Western Blot: ALDH2 Antibody (3F10) [NBP3-15140] - Positive WB detected in: Ntera-2 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver cell lysate. All lanes: ALDH2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57, 51 KDa. Observed band size: 57 KDa.")
![Immunohistochemistry: ALDH2 Antibody (3F10) [NBP3-15140] - IHC image of ALDH2 antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/ALDH2-Antibody-3F10-Immunohistochemistry-NBP3-15140-img0001.jpg)
![Western Blot: ALDH2 Antibody (SR2007) [NBP3-21946] - Western blot analysis using NBP3-21946 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21946_rabbit-aldh2-mab-sr2007-57202316582173.jpg "Western Blot: ALDH2 Antibody (SR2007) [NBP3-21946] - Western blot analysis using NBP3-21946 in HepG2 cell lysate.")