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![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0007.jpg "Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-59320_mouse-monoclonal-sur1-antibody-s289-16-310202416114773.jpg)
![Western Blot: SUR1 Antibody [NBP1-59778] - 293T cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/SUR1-Antibody-Western-Blot-NBP1-59778-img0005.jpg "Western Blot: SUR1 Antibody [NBP1-59778] - 293T cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: SUR1 Antibody [NBP1-59778] - Human small intestine tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-Paraffin-NBP1-59778-img0004.jpg)
![Western Blot: SUR1 Antibody [NBP3-12497] - 1:10000 antibody dilution in DiluOBuffer. Apparent MW is 18.5 KDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-Western-Blot-NBP3-12497-img0001.jpg "Western Blot: SUR1 Antibody [NBP3-12497] - 1:10000 antibody dilution in DiluOBuffer. Apparent MW is 18.5 KDa.")
![Western Blot: SUR1 Antibody [NBP1-00138] - (0.5ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SUR1-Antibody-Western-Blot-NBP1-00138-img0002.jpg "Western Blot: SUR1 Antibody [NBP1-00138] - (0.5ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Sandwich ELISA: SUR1 Antibody (3E1) [H00006833-M02] - Detection limit for recombinant GST tagged ABCC8 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SUR1-Antibody-3E1-Sandwich-ELISA-H00006833-M02-img0001.jpg "Sandwich ELISA: SUR1 Antibody (3E1) [H00006833-M02] - Detection limit for recombinant GST tagged ABCC8 is 0.03 ng/ml as a capture antibody.")
![ELISA: Human SUR1 ELISA Kit (Colorimetric) [NBP3-27490] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27490_human-sur1-elisa-kit-colorimetric-114202410295927.png "ELISA: Human SUR1 ELISA Kit (Colorimetric) [NBP3-27490] - Standard Curve Reference")
![ELISA: Human SUR1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31125] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31125_human-sur1-ready-to-use-elisa-kit-colorimetric-20620241533972.png "ELISA: Human SUR1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31125] - Standard Curve Reference")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody [NBP2-34077] - Staining of human cell line U-2 OS shows localization to nucleoli, cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34077-img0008.jpg "Immunocytochemistry/Immunofluorescence: SUR1 Antibody [NBP2-34077] - Staining of human cell line U-2 OS shows localization to nucleoli, cytosol & the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-Paraffin-NBP2-34077-img0013.jpg)
![Immunohistochemistry: SUR1 Antibody [NBP2-34077] - IHC using NBP2-34077 at a dilution of 1:50 on FFPE human placenta. Primary antibody was incubated overnight at 4 C, then secondary HRP conjugate applied for 1 hour RT and visualized with DAB. HIER performed with citrate buffer pH 6.0 at 95 C for 15 minutes. Antibody incubation with 10x immunogenic peptide shows no staining. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-NBP2-34077-img0007.jpg)
![Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-Paraffin-NBP2-34077-img0011.jpg)
![Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-Paraffin-NBP2-34077-img0012.jpg)
![Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SUR1-Antibody-Immunohistochemistry-Paraffin-NBP2-34077-img0014.jpg)