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![Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - SNF2H Antibody [NB100-55310] - Detection of Human SNF2h/ISWI on HeLa whole cell lysate using NB100-55310. SNF2h/ISWI was also immunoprecipitated by rabbit anti-SNF2h/ISWI antibodies BL4495 and NB100-55311.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NB100-55310-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - SNF2H Antibody [NB100-55310] - Detection of Human SNF2h/ISWI on HeLa whole cell lysate using NB100-55310. SNF2h/ISWI was also immunoprecipitated by rabbit anti-SNF2h/ISWI antibodies BL4495 and NB100-55311.")
![<b>Independent Antibodies Validation. </b>Western Blot: SMARCA5/SNF2H Antibody [NB100-55310] - WB analysis of SNF2H in Mouse Stem Cell lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NB100-55310-img0003.jpg)
![Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMARCA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NBP1-89692-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMARCA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody [NBP1-89692] - Staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89692-img0008.jpg)
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunohistochemistry-Paraffin-NBP1-89692-img0001.jpg)
![Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NBP1-89692-img0006.jpg)
![Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NBP1-89692-img0007.jpg)
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692] -Staining of human tonsil shows strong nuclear positivity in germinal and non-germinal center cells](http://images.novusbio.com/fullsize/antibody/nbp1-89692_rabbit-polyclonal-smarca5-snf2h-antibody-immunohistochemistry-paraffin-276202312526..jpg)
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692] -Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-89692_rabbit-polyclonal-smarca5-snf2h-antibody-immunohistochemistry-paraffin-2762023125316..jpg)
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692] -Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-89692_rabbit-polyclonal-smarca5-snf2h-antibody-immunohistochemistry-paraffin-2762023125421..jpg)
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0005.jpg "Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Immunofluorescence analysis of U-2 OS cells using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Immunocytochemistry-Immunofluorescence-NBP3-16216-img0003.jpg)
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of various cell lines, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Immunofluorescence analysis of C6 cells using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Immunocytochemistry-Immunofluorescence-NBP3-16216-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Immunofluorescence analysis of NIH-3T3 cells using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Immunocytochemistry-Immunofluorescence-NBP3-16216-img0002.jpg)
![Immunoprecipitation: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SMARCA5/SNF2H antibody (NBP3-16216). Western blot was performed from the immunoprecipitate using SMARCA5/SNF2H antibody (NBP3-16216) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-6H8I7-Immunoprecipitation-NBP3-16216-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Immunocytochemistry-Immunofluorescence-H00008467-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Immunohistochemistry-Paraffin-H00008467-M02-img0005.jpg)
![Sandwich ELISA: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Detection limit for recombinant GST tagged SMARCA5 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-3F4-Sandwich-ELISA-H00008467-M02-img0006.jpg)
![Western Blot: SMARCA5/SNF2H Antibody [NBP3-12952] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:10000.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Western-Blot-NBP3-12952-img0001.jpg "Western Blot: SMARCA5/SNF2H Antibody [NBP3-12952] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody [NBP3-12952] - SMARCA5/SNF2H antibody detects SMARCA5/SNF2H protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA5/SNF2H protein stained by SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12952-img0003.jpg)
![Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP3-12952] - SMARCA5/SNF2H antibody detects SMARCA5/SNF2H protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. SMARCA5/SNF2H antibody (NBP3-12952) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunohistochemistry-Paraffin-NBP3-12952-img0002.jpg)
![Western Blot: SMARCA5/SNF2H Antibody (S05-9D5) [NBP3-15093] - Western blot detection of SMARCA5/SNF2H in K562, C6, 3T3, Hela cell lysates using SMARCA5/SNF2H Rabbit mAb (1:1000 diluted). Predicted band size: 122kDa. Observed band size: 122kDa.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-S05-9D5-Western-Blot-NBP3-15093-img0001.jpg "Western Blot: SMARCA5/SNF2H Antibody (S05-9D5) [NBP3-15093] - Western blot detection of SMARCA5/SNF2H in K562, C6, 3T3, Hela cell lysates using SMARCA5/SNF2H Rabbit mAb (1:1000 diluted). Predicted band size: 122kDa. Observed band size: 122kDa.")
![Western Blot: SMARCA5/SNF2H Antibody (JE40-44) [NBP3-32963] - Western blot analysis of SMARCA5/SNF2H on different lysates with Rabbit anti-SMARCA5/SNF2H antibody (NBP3-32963) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: A549 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 122 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32963_rabbit-smarca5-snf2h-mab-je40-44-288202412493474.jpg "Western Blot: SMARCA5/SNF2H Antibody (JE40-44) [NBP3-32963] - Western blot analysis of SMARCA5/SNF2H on different lysates with Rabbit anti-SMARCA5/SNF2H antibody (NBP3-32963) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: A549 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 122 kDa Observed band size: 122 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Chromatin Immunoprecipitation: SMARCA5/SNF2H Antibody [NBP3-10883] - Chromatin Immunoprecipitation (ChIP) using NBP3-10883 and HCT116 Cells](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Chromatin-Immunoprecipitation-NBP3-10883-img0001.jpg "Chromatin Immunoprecipitation: SMARCA5/SNF2H Antibody [NBP3-10883] - Chromatin Immunoprecipitation (ChIP) using NBP3-10883 and HCT116 Cells")
![Western Blot: SNF2H Overexpression Lysate (Adult Normal) [NBL1-16228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNF2H.](http://images.novusbio.com/fullsize/SNF2H-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16228-img0002.jpg "Western Blot: SNF2H Overexpression Lysate (Adult Normal) [NBL1-16228] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNF2H.")
![Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311] - Detection of Human SNF2h/ISWI by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI antibody NB100-55311 used for IP at 3 mcg/mg lysate. SNF2h/ISWI was also immunoprecipitated by another rabbit anti-SNF2h/ISWI antibody and NB100-55310, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SMARCA5-SNF2H-Antibody-Immunoprecipitation-NB100-55311-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311] - Detection of Human SNF2h/ISWI by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI antibody NB100-55311 used for IP at 3 mcg/mg lysate. SNF2h/ISWI was also immunoprecipitated by another rabbit anti-SNF2h/ISWI antibody and NB100-55310, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.")