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![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Immunohistochemistry analysis in human smooth muscle and liver tissues using Anti-SLMAP antibody. Corresponding SLMAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-NBP1-81397-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Immunohistochemistry analysis in human smooth muscle and liver tissues using Anti-SLMAP antibody. Corresponding SLMAP RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human liver, lymph node, smooth muscle and testis using Anti-SLMAP antibody NBP1-81397 (A) shows similar protein distribution across tissues to independent antibody NBP1-81398 (B).](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0017.jpg)
![Western Blot: SLMAP Antibody [NBP1-81397] - SLMAP transgene protein expression in Tg mice. Western blot of heart lysate from Wt and Tg mice with anti-myc in high (H), moderate (M), and low (L) SLMAP expression, GAPDH was used as loading control. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0214669) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Western-Blot-NBP1-81397-img0027.jpg)
![Immunocytochemistry/Immunofluorescence: SLMAP Antibody [NBP1-81397] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81397-img0009.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0026.jpg)
![Western Blot: SLMAP Antibody [NBP1-81397] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Western-Blot-NBP1-81397-img0015.jpg)
![Western Blot: SLMAP Antibody [NBP1-81397] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Western-Blot-NBP1-81397-img0016.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human testis.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0019.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human lymph node.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0020.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human liver using Anti-SLMAP antibody NBP1-81397.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0021.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human smooth muscle using Anti-SLMAP antibody NBP1-81397.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0022.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81397-img0025.jpg)
![Western Blot: SLMAP Antibody [NBP1-81397] - Postnatal expression of SLMAP3 in Tg mice.(A) Schematic representation of the SLMAP3 transgene construct comprising the forkhead associated domain (FHA), the leucine zipper coiled-coil region (LZ), & the transmembrane domain 2 (TM2) in frame with 6-myc tag, driven by alpha -MHC promoter. (B) Number of SLMAP3 transgene copies in hearts. DNA was isolated from hearts of mice & qRT-PCR was performed with appropriate primers to assess copies of Myc-SLMAP3 transgene. (C) SLMAP3 transgene protein expression in Tg mice. Western blot of heart lysate from Wt & Tg mice with anti-myc in high (H), moderate (M), & low (L) SLMAP3-six myc expression, GAPDH was used as loading control. (D) Endogenous SLMAP isoforms in Wt & Tg myocardium. Western blots of SLMAP isoforms in Wt & Tg mice at 5 weeks of age with anti-SLMAP (~35 kDa, ~43 kDa, ~81 kDa, ~91 kDa, & 110 kDa) & anti-myc antibodies (~110 kDa). SLMAP3 image was acquired with a longer exposure of the same membrane. (E) Quantification & fold change of protein expression levels of SLMAP1 (~35 kDa) & SLMAP2 (~43 kDa) isoforms in Tg mice compared to Wt age-matched littermates at 5 weeks of age; Wt corresponds to 1 (100%), n = 3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30934005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81397_rabbit-polyclonal-slmap-antibody-310202416121919.jpg)
![Western Blot: SLMAP Antibody [NBP1-81397] - Expression of calcium handling proteins & their phosphorylation in microsomes.(A) Western blot analysis of SERCA2a protein expression in microsomal fractions & total protein acquired by stain-free. (B) Quantification of SERCA2a in microsomal fractions normalized to total protein assessed by stain free technology in Wt (n = 4) & Tg (n = 7) mice. (C) Western blots for total ryanodine receptor 2 (t-RyR2), it’s phosphorylation on serine 2808 (p-RyR2 ser 2808), calsequestrin (CSQ), total phospholamban (t-PLN) & it’s phosphorylation on serine 16 (p-PLN ser16) in microsomal fractions from 5 weeks old Tg & Wt mice hearts. Anti-calreticulin was used as loading control. Tg SLMAP3 was detected by anti-myc (Myc-SLMAP3). (D) Quantification of RyR2, CSQ, & PLN (monomeric) protein expression in Wt & Tg mice. *p<0.05, #p = 0.053, n (Wt) = 5, n (Tg) = 4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30934005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81397_rabbit-polyclonal-slmap-antibody-310202416175231.jpg)
![Western Blot: SLMAP Antibody [NBP1-81397] - Expression of calcium handling proteins & their phosphorylation in microsomes.(A) Western blot analysis of SERCA2a protein expression in microsomal fractions & total protein acquired by stain-free. (B) Quantification of SERCA2a in microsomal fractions normalized to total protein assessed by stain free technology in Wt (n = 4) & Tg (n = 7) mice. (C) Western blots for total ryanodine receptor 2 (t-RyR2), it’s phosphorylation on serine 2808 (p-RyR2 ser 2808), calsequestrin (CSQ), total phospholamban (t-PLN) & it’s phosphorylation on serine 16 (p-PLN ser16) in microsomal fractions from 5 weeks old Tg & Wt mice hearts. Anti-calreticulin was used as loading control. Tg SLMAP3 was detected by anti-myc (Myc-SLMAP3). (D) Quantification of RyR2, CSQ, & PLN (monomeric) protein expression in Wt & Tg mice. *p<0.05, #p = 0.053, n (Wt) = 5, n (Tg) = 4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30934005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81397_rabbit-polyclonal-slmap-antibody-310202416163787.jpg)
![Western Blot: SLMAP Antibody [NBP1-81398] - Analysis using Anti-SLMAP antibody NBP1-81398 (A) shows similar pattern to independent antibody NBP1-81397 (B).](http://images.novusbio.com/fullsize/SLMAP-Antibody-Western-Blot-NBP1-81398-img0013.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SLMAP Antibody [NBP1-81398] - Analysis using Anti-SLMAP antibody NBP1-81398 (A) shows similar pattern to independent antibody NBP1-81397 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SLMAP Antibody [NBP1-81398] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81398-img0007.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human smooth muscle using Anti-SLMAP antibody NBP1-81398.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0017.jpg)
![Western Blot: SLMAP Antibody [NBP1-81398] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/SLMAP-Antibody-Western-Blot-NBP1-81398-img0005.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0009.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining in human smooth muscle and liver tissues using anti-SLMAP antibody. Corresponding SLMAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human liver, lymph node, smooth muscle and testis using Anti-SLMAP antibody NBP1-81398 (A) shows similar protein distribution across tissues to independent antibody NBP1-81397 (B).](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0012.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human testis.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0014.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human lymph node.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0015.jpg)
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81398] - Staining of human liver using Anti-SLMAP antibody NBP1-81398.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP1-81398-img0016.jpg)
![Western Blot: SLMAP Antibody (2A7) [H00007871-M08] - Analysis of SLMAP expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/SLMAP-Antibody-2A7-Western-Blot-H00007871-M08-img0005.jpg "Western Blot: SLMAP Antibody (2A7) [H00007871-M08] - Analysis of SLMAP expression in A-431 (Cat # L015V1).")
![Immunohistochemistry-Paraffin: SLMAP Antibody (2A7) [H00007871-M08] - Analysis of monoclonal antibody to SLMAP on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/SLMAP-Antibody-2A7-Immunohistochemistry-Paraffin-H00007871-M08-img0003.jpg)
![ELISA: SLMAP Antibody (2A7) [H00007871-M08] - Detection limit for recombinant GST tagged SLMAP is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SLMAP-Antibody-2A7-ELISA-H00007871-M08-img0006.jpg)
![Western Blot: SLMAP Antibody [NBP3-15511] - Western blot analysis of extracts of various cell lines, using SLMAP Rabbit pAb (NBP3-15511) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Western-Blot-NBP3-15511-img0001.jpg "Western Blot: SLMAP Antibody [NBP3-15511] - Western blot analysis of extracts of various cell lines, using SLMAP Rabbit pAb (NBP3-15511) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: SLMAP Overexpression Lysate (Adult Normal) [NBP2-07525] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLMAP.](http://images.novusbio.com/fullsize/SLMAP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07525-img0001.jpg "Western Blot: SLMAP Overexpression Lysate (Adult Normal) [NBP2-07525] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLMAP.")
![Immunohistochemistry-Paraffin: SLMAP Antibody [NBP3-06131] - Staining of human SLMAP in human testis with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SLMAP-Antibody-Immunohistochemistry-Paraffin-NBP3-06131-img0001.jpg "Immunohistochemistry-Paraffin: SLMAP Antibody [NBP3-06131] - Staining of human SLMAP in human testis with rabbit polyclonal antibody at 1:500 dilution.")