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![Western Blot: RPL5 Antibody [NBP1-31413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPL5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-31413-img0018.jpg "Western Blot: RPL5 Antibody [NBP1-31413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPL5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: RPL5 Antibody [NBP1-31413] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 protein stained by RPL5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31413-img0013.jpg)
![Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Mouse hind brain. RPL5 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunohistochemistry-Paraffin-NBP1-31413-img0012.jpg)
![Western Blot: RPL5 Antibody [NBP1-31413] - A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE RPL5 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-31413-img0007.jpg)
![Western Blot: RPL5 Antibody [NBP1-31413] - A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-31413-img0010.jpg)
![Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Gastric CA N87 xenograft, using RPL5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunohistochemistry-Paraffin-NBP1-31413-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RPL5 Antibody [NBP1-31413] - Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice. Image collected and cropped by CiteAb from the following publication (www.discoveriesjournals.org/discoveries/D.2019.03.DI-Kazerounian-Beggs.DOI) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPL5-Antibody-Knockdown-Validated-NBP1-31413-img0016.jpg)
![Western Blot: RPL5 Antibody [NBP1-31413] - A. Methylcellulose colony assay on bone marrow cells from doxycycline treated shRNA Rpl5 & control mice (n=3), (CFU-E, P=0.0003; BFU-E, P=1; GFU-GM, P=0.3); B. FACS analysis of bone marrow cells from shRNA Rpl5 & control mice. Total bone marrow cells from shRNA Rpl5 (B.1) & control (B.2) mice; viable cells were gated for analysis & sorting. We used the cell-surface markers CD71 (Y-axis) & Ter119 (X-axis) to separate mature erythroids & erythroid precursors. The most mature erythroblasts are CD71lowTer119high, while the erythroid precursors are CD71highTer119intermediate cells. In our FACS analysis the gated population comprises CD71highTer119med cells (P4) & CD71highTer119high cells (P5) from shRNA Rpl5 (B.3 & B.5) & control (B4 & B6) mice; the results here are collected from one mouse per group & are representative of 6 independent experiments; in each experiment, cells were collected from 1-3 mice for each group & sorted independently; C. Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice; D. Western Blot analysis of GATA1 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression of GATA1 in the shRNA Rpl5 group compared to the control mice. In these experiments, GAPDH was used as a loading control, & they are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32309614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31413_rabbit-polyclonal-rpl5-antibody-310202416171434.jpg)
![Western Blot: RPL5 Antibody [NBP2-22285] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-RPL5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence withan exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP2-22285-img0013.jpg "Western Blot: RPL5 Antibody [NBP2-22285] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-RPL5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence withan exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (NBP2-22285). Detection: DAB](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunocytochemistry-NBP2-22285-img0010.jpg)
![Immunohistochemistry-Paraffin: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (NBP2-22285). Detection: DAB](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunohistochemistry-Paraffin-NBP2-22285-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RPL5 Antibody [NBP2-22285] - Detection of human RPL5 by western blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody NBP2-22285 used for IP at 3 ug per reaction. RPL5 was also immunoprecipitated by another rabbit anti-RPL5 antibody. For blotting immunoprecipitated RPL5, NBP2-22285 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunoprecipitation-NBP2-22285-img0012.jpg)
![Western Blot: RPL5 Antibody [NBP1-92347] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-92347-img0005.jpg "Western Blot: RPL5 Antibody [NBP1-92347] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-92347] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunohistochemistry-Paraffin-NBP1-92347-img0006.jpg)
![Western Blot: RPL5 Antibody [NBP1-92347] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-92347-img0004.jpg)
![Western Blot: RPL5 Antibody [NBP1-57126] - Sample Type: Arabidopsis thaliana extract (30ug) Primary Diltution: 1:1000 Secondary Antibody: anti-rabbit HRP Secondary Dilution: 1:15,000 Exposure Time: 1 minute Image Submitted By: Anonymous.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-57126-img0005.jpg "Western Blot: RPL5 Antibody [NBP1-57126] - Sample Type: Arabidopsis thaliana extract (30ug) Primary Diltution: 1:1000 Secondary Antibody: anti-rabbit HRP Secondary Dilution: 1:15,000 Exposure Time: 1 minute Image Submitted By: Anonymous.")
![Western Blot: RPL5 Antibody [NBP1-57126] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-57126-img0003.jpg)
![Western Blot: RPL5 Antibody [NBP2-68710] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP2-68710-img0001.jpg "Western Blot: RPL5 Antibody [NBP2-68710] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: RPL5 Antibody [NBP2-68710] - Staining of human cell line A-431 shows localization to nucleoli, cytosol & endoplasmic reticulum.](http://images.novusbio.com/fullsize/RPL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68710-img0002.jpg)
![Western Blot: RPL5 Antibody [NBP1-74074] - Titration: 1.0 ug/ml Positive Control: Mouse Heart.](http://images.novusbio.com/fullsize/RPL5-Antibody-Western-Blot-NBP1-74074-img0002.jpg "Western Blot: RPL5 Antibody [NBP1-74074] - Titration: 1.0 ug/ml Positive Control: Mouse Heart.")
![Western Blot: RPL5 Overexpression Lysate (Adult Normal) [NBL1-15541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPL5.](http://images.novusbio.com/fullsize/RPL5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15541-img0002.jpg "Western Blot: RPL5 Overexpression Lysate (Adult Normal) [NBL1-15541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPL5.")
![SDS-Page: RPL5 Recombinant Protein [NBP2-51794] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/RPL5-Recombinant-Protein-SDS-Page-NBP2-51794-img0001.jpg "SDS-Page: RPL5 Recombinant Protein [NBP2-51794] - 15% SDS Page (3 ug)")