RPL5 Antibody Summary
Immunogen |
The immunogen this antibody was made to, maps to a region between residue 247 and 297 of human Ribosomal Protein L5 using the numbering given in entry NP_000960.2 (GeneID 6125). |
Predicted Species |
Rat (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
RPL5 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:500 - 1:2000
- Immunohistochemistry-Paraffin 1:500 - 1:2000
- Immunoprecipitation 2 - 5 ug/mg
- Western Blot 1:2000 - 1:10000
|
Application Notes |
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
Preservative |
0.09% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for RPL5 Antibody
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Secondary Antibodies
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