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![Western Blot: RECQ1 Antibody [NB100-619] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RecQ1 antibody NB100-619 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Western-Blot-NB100-619-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: RECQ1 Antibody [NB100-619] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RecQ1 antibody NB100-619 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: RECQ1 Antibody [NB100-619] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 at 1:5000 (0.2 ug/mL). Detection: DAB](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunohistochemistry-NB100-619-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RECQ1 Antibody [NB100-619] - Detection of human RecQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RecQ1 antibody NB100-619 used for IP at 6 ug per reaction. RecQ1 was also immunoprecipitated by rabbit anti-RecQ1 antibody NB100-618. For blotting immunoprecipitated RecQ1, NB100-619 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunoprecipitation-NB100-619-img0009.jpg)
![Western Blot: RECQ1 Antibody [NB100-618] - Detection of human and mouse RecQ1 by Western blot using NB100-618 and human RecQ1 by immunoprecipitation. RecQ1 was successfully immunoprecipitated by NB100-619.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Western-Blot-NB100-618-img0001.jpg "Western Blot: RECQ1 Antibody [NB100-618] - Detection of human and mouse RecQ1 by Western blot using NB100-618 and human RecQ1 by immunoprecipitation. RecQ1 was successfully immunoprecipitated by NB100-619.")
![Immunohistochemistry-Paraffin: RECQ1 Antibody [NB100-618] - Human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti-RecQ1 used at a dilution of 1:200 (1ug/ml). detection: DAB](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunohistochemistry-Paraffin-NB100-618-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RECQ1 Antibody [NBP1-87803] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87803-img0007.jpg "Immunocytochemistry/Immunofluorescence: RECQ1 Antibody [NBP1-87803] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunohistochemistry-Paraffin-NBP1-87803-img0009.jpg)
![Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunohistochemistry-Paraffin-NBP1-87803-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803] - Staining in human lymph node and pancreas tissues using anti-RECQL antibody. Corresponding RECQL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunohistochemistry-Paraffin-NBP1-87803-img0010.jpg)
![Western Blot: RECQ1 Antibody [NBP2-57572] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Western-Blot-NBP2-57572-img0002.jpg "Western Blot: RECQ1 Antibody [NBP2-57572] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: RECQ1 Antibody [NBP2-57572] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RECQ1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57572-img0001.jpg)
![SDS-Page: Recombinant Human RECQ1 Protein [H00005965-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005965-Q01-1.jpg "SDS-Page: Recombinant Human RECQ1 Protein [H00005965-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: RECQ1 Overexpression Lysate (Adult Normal) [NBL1-15264] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RECQ1.](http://images.novusbio.com/fullsize/RECQ1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15264-img0002.jpg "Western Blot: RECQ1 Overexpression Lysate (Adult Normal) [NBL1-15264] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RECQ1.")
![Sandwich ELISA: RECQ1 Antibody (4D5) [H00005965-M07] - Detection limit for recombinant GST tagged RECQL is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RECQ1-Antibody-4D5-Sandwich-ELISA-H00005965-M07-img0001.jpg "Sandwich ELISA: RECQ1 Antibody (4D5) [H00005965-M07] - Detection limit for recombinant GST tagged RECQL is 3 ng/ml as a capture antibody.")