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![Western Blot: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Western-Blot-NBP2-45515-img0009.jpg "Western Blot: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0008.jpg)
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0002.jpg)
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0003.jpg)
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0004.jpg)
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0005.jpg)
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0006.jpg)
![Immunohistochemistry: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunohistochemistry-NBP2-45515-img0007.jpg)
![Immunofluorescence: GRF2 Antibody (OTI2F5) [NBP2-45515] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GRF2.](http://images.novusbio.com/fullsize/GRF2-Antibody-OTI2F5-Immunofluorescence-NBP2-45515-img0001.jpg)
![Western Blot: GRF2 Antibody [NBP1-88266] - Analysis in control (vector only transfected HEK293T lysate) and RAPGEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/GRF2-Antibody-Western-Blot-NBP1-88266-img0006.jpg "Western Blot: GRF2 Antibody [NBP1-88266] - Analysis in control (vector only transfected HEK293T lysate) and RAPGEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/GRF2-Antibody-Immunohistochemistry-Paraffin-NBP1-88266-img0008.jpg)
![Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/GRF2-Antibody-Immunohistochemistry-Paraffin-NBP1-88266-img0001.jpg)
![Immunohistochemistry-Paraffin: GRF2 Antibody [NBP1-88266] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/GRF2-Antibody-Immunohistochemistry-Paraffin-NBP1-88266-img0007.jpg)
![Western Blot: GRF2 Antibody [NBP3-13284] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GRF2 antibody (NBP3-13284) diluted at 1:1000.](http://images.novusbio.com/fullsize/GRF2-Antibody-Western-Blot-NBP3-13284-img0001.jpg "Western Blot: GRF2 Antibody [NBP3-13284] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GRF2 antibody (NBP3-13284) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: GRF2 Antibody [NBP3-13284] - GRF2 antibody detects GRF2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. GRF2 stained by GRF2 antibody (NBP3-13284) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRF2-Antibody-Immunohistochemistry-Paraffin-NBP3-13284-img0003.jpg)
![Immunohistochemistry-Paraffin: GRF2 Antibody [NBP3-13284] - GRF2 antibody detects GRF2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. GRF2 stained by GRF2 antibody (NBP3-13284) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRF2-Antibody-Immunohistochemistry-Paraffin-NBP3-13284-img0002.jpg)
![Western Blot: GRF2 Antibody [NBP1-06500] - Detection of Human C3G by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GRF2-Antibody-Western-Blot-NBP1-06500-img0002.jpg "Western Blot: GRF2 Antibody [NBP1-06500] - Detection of Human C3G by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: GRF2 Antibody [NBP1-06500] - Whole cell lysate from HeLa cells. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06501.](http://images.novusbio.com/fullsize/GRF2-Antibody-Western-Blot-NBP1-06500-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: GRF2 Antibody [NBP1-06500] - Detection of human C3G by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for IP at 6 ug per reaction. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06501. For blotting immunoprecipitated C3G, NBP1-06500 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GRF2-Antibody-Immunoprecipitation-NBP1-06500-img0003.jpg)
![Western Blot: GRF2 Antibody (3D10) [H00002889-M01] - Analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GRF2-Antibody-3D10-Western-Blot-H00002889-M01-img0003.jpg "Western Blot: GRF2 Antibody (3D10) [H00002889-M01] - Analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: GRF2 Antibody (3D10) [H00002889-M01] - Detection limit for recombinant GST tagged RAPGEF1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GRF2-Antibody-3D10-Sandwich-ELISA-H00002889-M01-img0002.jpg)
![Western Blot: GRF2 Antibody [NBP1-06501] - Whole cell lysate from HeLa cells. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06500.](http://images.novusbio.com/fullsize/GRF2-Antibody-Western-Blot-NBP1-06501-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GRF2 Antibody [NBP1-06501] - Whole cell lysate from HeLa cells. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06500.")
![Western Blot: GRF2 Antibody [NBP1-58306] - Human Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/GRF2-Antibody-Western-Blot-NBP1-58306-img0002.jpg "Western Blot: GRF2 Antibody [NBP1-58306] - Human Brain lysate, concentration 0.2-1 ug/ml.")
![Western Blot: GRF2 Overexpression Lysate (Adult Normal) [NBL1-15151] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRF2.](http://images.novusbio.com/fullsize/GRF2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15151-img0002.jpg "Western Blot: GRF2 Overexpression Lysate (Adult Normal) [NBL1-15151] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRF2.")