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PVRL2 Products By Gene Name

4 Results for "PVRL2"

AF2229

Nectin-2/CD112 Antibody [Unconjugated]

(10 Publications)

100 μg

AF2229

Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nectin-2/CD112 at approximately 60-75 kDa (as indicated). Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line are shown as negative controls. GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Nectin-2/CD112 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nectin‑2/CD112 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Nectin‑2/CD112 at approximately 71-86 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

B3GNT2 disrupts ligand–receptor interactions between tumor and T cells.a Cell survival against T cell cytotoxicity (top) and T cell IFN gamma secretion (bottom) in A375 cells overexpressing B3GNT2 or GFP that have been treated with different concentrations of kifunensine. Kifunensine was used to pretreat A375 cells and was present during co-culture with T cells at E:T ratio of 3. Kifunensine-treated cells that were co-cultured with ESO T cells were compared to kifunensine-treated cells cultured in parallel without T cells to determine percent survival. N = 6. Two-tailed t tests were performed. b Tomato lectin IP of A375 cells overexpressing GFP or B3GNT2 followed by western blot for different B3GNT2 target proteins. 2% of the input and no lectin IP controls are shown. Data are representative of two independent experiments. c Western blots of A375 cells overexpressing B3GNT2 or GFP that were treated with kifunensine (KIF) or benzyl-2-acetamido-2-deoxy-alpha -D-galactopyranoside (BAG) to remove N- or O-glycosylation respectively. Data are representative of two independent experiments. d Histograms (top) and corresponding median fluorescence intensity (MFI; bottom) showing binding of recombinant T cell proteins to A375 cells measured by flow cytometry. A375 cells were overexpressing GFP or B3GNT2 and treated with KIF or BAG. N = 3. Two-tailed t tests were performed. e Schematic showing the tumor cell surface ligands and receptors modified by B3GNT2 to disrupt interactions with T cells that mediate cytotoxicity. All values are mean ± s.e.m. ns not significant. Source data are provided in Source Data 4. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35338135), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2229-SP

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MAB3869

Nectin-2/CD112 Antibody (829038)

(7 Publications)

100 μg

MAB3869

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Nectin‑2/CD112 Monoclonal Antibody (Catalog # MAB3869, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3869U-100UG
Alexa Fluor 405	FAB3869V-100UG
Alexa Fluor 488	FAB3869G
Alexa Fluor 532	FAB3869X
Alexa Fluor 594	FAB3869T-100UG
Alexa Fluor 647	FAB3869R-100UG
Alexa Fluor 700	FAB3869N-100UG
Alexa Fluor 750	FAB3869S-100UG
Allophycocyanin	FAB3869A
Allophycocyanin/Cy7	FAB3869APCCY7
Biotin	FAB3869B
CoraFluor 1	FAB3869CL1
DyLight 350	FAB3869D
DyLight 405	FAB3869E
DyLight 488	FAB3869K
DyLight 550	FAB3869L
DyLight 594	FAB3869M
DyLight 650	FAB3869W
DyLight 680	FAB3869Y
DyLight 755	FAB3869Z
FITC	FAB3869F
HRP	FAB3869H
Janelia Fluor 525	FAB3869JF525
Janelia Fluor 549	FAB3869I
Janelia Fluor 646	FAB3869J
Janelia Fluor 669	FAB3869JF669
PE/Atto594	FAB3869PEATT594
PE/Cy5.5	FAB3869PECY55
PE/Cy7	FAB3869PECY7
PerCP	FAB3869C
Phycoerythrin	FAB3869P
mFluor Violet 450 SE	FAB3869MFV450
mFluor Violet 500 SE	FAB3869MFV500
mFluor Violet 610 SE	FAB3869MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3869P
Carrier Free	FAB3869A
Carrier Free	FAB3869G
Carrier Free	MAB3869-SP
Carrier Free	FAB3869V-100UG
Carrier Free	FAB3869U-100UG
Carrier Free	FAB3869T-100UG
Carrier Free	FAB3869S-100UG
Carrier Free	FAB3869R-100UG
Carrier Free	FAB3869N-100UG

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AF3869

Nectin-2/CD112 Antibody

100 μg

AF3869

Western blot shows lysates of C2C12 mouse myoblast cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Nectin-2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nectin-2/CD112 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3869-SP

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FAB3869G

Nectin-2/CD112 Antibody (829038) [Alexa Fluor® 488]

(2 Publications)

100 Tests

FAB3869G

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Nectin-2/CD112 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3869G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Alexa Fluor 488

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3869U-100UG
Alexa Fluor 405	FAB3869V-100UG
Alexa Fluor 532	FAB3869X
Alexa Fluor 594	FAB3869T-100UG
Alexa Fluor 647	FAB3869R-100UG
Alexa Fluor 700	FAB3869N-100UG
Alexa Fluor 750	FAB3869S-100UG
Allophycocyanin	FAB3869A
Allophycocyanin/Cy7	FAB3869APCCY7
Biotin	FAB3869B
CoraFluor 1	FAB3869CL1
DyLight 350	FAB3869D
DyLight 405	FAB3869E
DyLight 488	FAB3869K
DyLight 550	FAB3869L
DyLight 594	FAB3869M
DyLight 650	FAB3869W
DyLight 680	FAB3869Y
DyLight 755	FAB3869Z
FITC	FAB3869F
HRP	FAB3869H
Janelia Fluor 525	FAB3869JF525
Janelia Fluor 549	FAB3869I
Janelia Fluor 585	FAB3869JF585
Janelia Fluor 635	FAB3869JF635
Janelia Fluor 646	FAB3869J
Janelia Fluor 669	FAB3869JF669
PE/Atto594	FAB3869PEATT594
PE/Cy5.5	FAB3869PECY55
PE/Cy7	FAB3869PECY7
PerCP	FAB3869C
Phycoerythrin	FAB3869P
mFluor Violet 450 SE	FAB3869MFV450
mFluor Violet 500 SE	FAB3869MFV500
mFluor Violet 610 SE	FAB3869MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB3869P
Carrier Free	MAB3869
Carrier Free	FAB3869A
Carrier Free	MAB3869-SP
Carrier Free	FAB3869V-100UG
Carrier Free	FAB3869U-100UG
Carrier Free	FAB3869T-100UG
Carrier Free	FAB3869S-100UG
Carrier Free	FAB3869R-100UG
Carrier Free	FAB3869N-100UG

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