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![Western Blot: Peroxiredoxin 1 Antibody [NBP1-82558] - Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PRDX1 antibody. Corresponding PRDX1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-82558-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Peroxiredoxin 1 Antibody [NBP1-82558] - Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PRDX1 antibody. Corresponding PRDX1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82558-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0014.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0011.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0012.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0013.jpg)
![Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-30973-img0014.jpg "Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-30973] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PRX I stained by PRX I antibody [N1C2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30973-img0010.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-30973] - Mouse lung. PRX I antibody [N1C2] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30973-img0009.jpg)
![Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-30973-img0006.jpg)
![Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-30973-img0007.jpg)
![Western Blot: Peroxiredoxin 1 Antibody [NBP1-30973] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-30973-img0012.jpg)
![Western Blot: Peroxiredoxin 1 Antibody (4B11-D10) [H00005052-M01] - PRDX1 monoclonal antibody (M01), clone 4B11-D10 Analysis of PRDX1 expression in HeLa.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-4B11-D10-Western-Blot-H00005052-M01-img0004.jpg "Western Blot: Peroxiredoxin 1 Antibody (4B11-D10) [H00005052-M01] - PRDX1 monoclonal antibody (M01), clone 4B11-D10 Analysis of PRDX1 expression in HeLa.")
![ELISA: Peroxiredoxin 1 Antibody (4B11-D10) [H00005052-M01] - Detection limit for recombinant GST tagged PRDX1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-4B11-D10-ELISA-H00005052-M01-img0005.jpg)
![Western Blot: Peroxiredoxin 1 Antibody [NBP1-06064] - Staining lysate of cell line HEK293 (A), A431 (B) and HepG2 (C). Antibody at 0.1 ug/mL. 35 ug protein in RIPA buffer. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-06064-img0001.jpg "Western Blot: Peroxiredoxin 1 Antibody [NBP1-06064] - Staining lysate of cell line HEK293 (A), A431 (B) and HepG2 (C). Antibody at 0.1 ug/mL. 35 ug protein in RIPA buffer. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-06064] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. Antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-06064-img0002.jpg)
![SDS-Page: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-Peroxiredoxin-1-His-Protein-SDS-Page-NBC1-18543-img0007.jpg "SDS-Page: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Functional: Recombinant Human Peroxiredoxin 1 His Protein [NBC1-18543] - The remaining peroxide content was determined by measuring the red-colored ferrithiocyanate complex formed as result of reaction at 475nm. delta H2O2 means the discrepancy of peroxide amount before and after reaction. This test was performed triplicate.](http://images.novusbio.com/fullsize/Recombinant-Human-Peroxiredoxin-1-His-Protein-Functional-NBC1-18543-img0006.jpg)
![SDS-Page: Recombinant Mouse Peroxiredoxin 1 Protein [NBP2-52340]](http://images.novusbio.com/fullsize/Recombinant-Mouse-Peroxiredoxin-1-Protein-SDS-Page-NBP2-52340-img0002.jpg "SDS-Page: Recombinant Mouse Peroxiredoxin 1 Protein [NBP2-52340]")
![Western Blot: Peroxiredoxin 1 Antibody [NBP2-99736] - Anti-Peroxiredoxin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP2-99736-img0004.jpg "Western Blot: Peroxiredoxin 1 Antibody [NBP2-99736] - Anti-Peroxiredoxin 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa")
![Immunoprecipitation: Peroxiredoxin 1 Antibody [NBP2-99736] - Peroxiredoxin 1 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. 2 ul anti-Peroxiredoxin 1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Peroxiredoxin 1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunoprecipitation-NBP2-99736-img0003.jpg)
![Western Blot: Peroxiredoxin 1 Antibody (3F8X3) [NBP3-16519] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 1 Rabbit mAb (NBP3-16519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-3F8X3-Western-Blot-NBP3-16519-img0001.jpg "Western Blot: Peroxiredoxin 1 Antibody (3F8X3) [NBP3-16519] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 1 Rabbit mAb (NBP3-16519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Peroxiredoxin 1 Overexpression Lysate (Adult Normal) [NBL1-14738] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 1.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14738-img0002.jpg "Western Blot: Peroxiredoxin 1 Overexpression Lysate (Adult Normal) [NBL1-14738] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 1.")
![Western Blot: Peroxiredoxin 1 Overexpression Lysate (Adult Normal) [NBP2-04806] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 1.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04806-img0001.jpg "Western Blot: Peroxiredoxin 1 Overexpression Lysate (Adult Normal) [NBP2-04806] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 1.")