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![Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PELP1-Antibody-Western-Blot-NB200-331-img0010.jpg "Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-NB200-331-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PELP1-Antibody-Western-Blot-NB200-331-img0008.jpg)
![Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Samples: FFPE sections of mouse renal cell carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR dilution of 1:2,500 (0.4ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-NB200-331-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PELP1 Antibody [NB200-331] - Detection of human PELP1/MNAR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 (lot NB200-331-3) used for IP at 6 ug per reaction. PELP1/MNAR was also immunoprecipitated by a previous lot of this antibody (lot NB200-331-2) and rabbit anti-PELP1/MNAR antibody NB110-40622. For blotting immunoprecipitated PELP1/MNAR, NB200-331 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunoprecipitation-NB200-331-img0009.jpg)
![Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-26820241582612.jpg "Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL")
![Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734] - Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-27820241521240.jpg)
![Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-298202414333664.jpg)
![Immunocytochemistry/ Immunofluorescence: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202410334017.jpg)
![Flow Cytometry: PELP1 Antibody (JE02-42) [NBP3-32734] - Flow cytometric analysis of MCF7 cells labeling PELP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32734, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202416453251.jpg)
![Immunohistochemistry-Paraffin: PELP1 Antibody [NB110-40622] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiPELP1/MNAR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-Paraffin-NB110-40622-img0003.jpg "Immunohistochemistry-Paraffin: PELP1 Antibody [NB110-40622] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiPELP1/MNAR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PELP1 Antibody [NB110-40622] - Detection of Human PELP1/MNAR by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody NB110-40622 used for IP at 3 mcg/mg lysate. PELP1/MNAR was also immunoprecipitated by other rabbit anti-PELP1/MNAR antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunoprecipitation-NB110-40622-img0002.jpg)
![Western Blot: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-Western-Blot-NBP2-61778-img0003.jpg "Western Blot: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)")
![Flow Cytometry: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis of Hela cells using PELP1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-Flow-Cytometry-NBP2-61778-img0002.jpg)
![Western Blot: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis using PELP1 mAb against HEK293 (1) and PELP1 (AA: 1031-1180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-Western-Blot-NBP2-61778-img0004.jpg)
![ELISA: PELP1 Antibody (4F1D1) [NBP2-61778] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-ELISA-NBP2-61778-img0001.jpg)
![Western Blot: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-Western-Blot-NBP2-61777-img0003.jpg "Western Blot: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)")
![Western Blot: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis using PELP1 mAb against HEK293 (1) and PELP1 (AA: 1031-1180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-Western-Blot-NBP2-61777-img0004.jpg)
![ELISA: PELP1 Antibody (2D6D11) [NBP2-61777] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-ELISA-NBP2-61777-img0001.jpg)
![ELISA: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis of Hela cells using PELP1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-ELISA-NBP2-61777-img0002.jpg)
![Western Blot: PELP1 Antibody (6H4) [NBP3-15157] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate. All lanes: PELP1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 KDa. Observed band size: 160 KDa.](http://images.novusbio.com/fullsize/PELP1-Antibody-6H4-Western-Blot-NBP3-15157-img0003.jpg "Western Blot: PELP1 Antibody (6H4) [NBP3-15157] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate. All lanes: PELP1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 KDa. Observed band size: 160 KDa.")
![Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157] - IHC image of PELP1 antibody diluted at 1:100 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-6H4-Immunohistochemistry-NBP3-15157-img0002.jpg)
![Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157] - IHC image of PELP1 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-6H4-Immunohistochemistry-NBP3-15157-img0001.jpg)
![Western Blot: PELP1 Antibody (SR1228) [NBP3-21650] - Western blot analysis using NBP3-21650 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21650_rabbit-pelp1-mab-sr1228-672023106234.jpg "Western Blot: PELP1 Antibody (SR1228) [NBP3-21650] - Western blot analysis using NBP3-21650 in 293T cell lysate.")
![Immunohistochemistry: PELP1 Antibody (SR1228) [NBP3-21650] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21650.](http://images.novusbio.com/fullsize/nbp3-21650_rabbit-pelp1-mab-sr1228-5720231624741.jpg)
![Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PELP1-Antibody-9V9A5-Western-Blot-NBP3-16736-img0001.jpg "Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of Rat testis, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PELP1-Antibody-9V9A5-Western-Blot-NBP3-16736-img0002.jpg)
![Western Blot: PELP1 Antibody (1F7) [H00027043-M01-100ug] - Expression in PC-12.](http://images.novusbio.com/fullsize/PELP1-Antibody-1F7-Western-Blot-H00027043-M01-100ug-img0001.jpg "Western Blot: PELP1 Antibody (1F7) [H00027043-M01-100ug] - Expression in PC-12.")
![Sandwich ELISA: PELP1 Antibody (1F7) [H00027043-M01-100ug] - Detection limit for recombinant GST tagged PELP1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PELP1-Antibody-1F7-Sandwich-ELISA-H00027043-M01-100ug-img0002.jpg)
![Immunohistochemistry-Paraffin: PELP1 Antibody [NBP2-32565] - Staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-Paraffin-NBP2-32565-img0007.jpg "Immunohistochemistry-Paraffin: PELP1 Antibody [NBP2-32565] - Staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.")
![Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-56265] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56265-img0001.jpg "Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-56265] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")
![Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-58736] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58736-img0001.jpg "Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-58736] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")