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![Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-1F3-Western-Blot-NBP1-41190-img0003.jpg "Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - A. 293T whole cell lysate/extract. B. A431 whole cell lysate/extract. C. HeLa whole cell lysate/extract. D. HepG2 whole cell lysate/extract. DNA ligase III dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-1F3-Western-Blot-NBP1-41190-img0002.jpg)
![Western Blot: DNA Ligase III Antibody [NBP2-92719] - Analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Western-Blot-NBP2-92719-img0005.jpg "Western Blot: DNA Ligase III Antibody [NBP2-92719] - Analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody [NBP2-92719] - Analysis of PC-12 cells using LIG3 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92719-img0011.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase III Antibody [NBP2-92719] - Immunohistochemistry of paraffin-embedded human colon using DNA Ligase III Rabbit pAb (NBP2-92719) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunohistochemistry-Paraffin-NBP2-92719-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody [NBP2-92719] - Analysis of NIH/3T3 cells using LIG3 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92719-img0010.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase III Antibody [NBP2-92719] - Mouse kidney using LIG3 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunohistochemistry-Paraffin-NBP2-92719-img0006.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase III Antibody [NBP2-92719] - Rat brain using LIG3 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunohistochemistry-Paraffin-NBP2-92719-img0007.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase III Antibody [NBP2-92719] - Rat liver using LIG3 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunohistochemistry-Paraffin-NBP2-92719-img0008.jpg)
![Immunohistochemistry-Paraffin: DNA Ligase III Antibody [NBP2-92719] - Rat ovary using LIG3 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunohistochemistry-Paraffin-NBP2-92719-img0009.jpg)
![Western Blot: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25441_rabbit-dna-ligase-iii-mab-hl2280-azide-and-bsa-free-2120249422676.jpg "Western Blot: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - DNA ligase III antibody [HL2280] detects DNA ligase III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. DNA ligase III stained by DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25441_rabbit-dna-ligase-iii-mab-hl2280-azide-and-bsa-free-2120249425423.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - DNA ligase III antibody [HL2280] detects DNA ligase III protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNA ligase III stained by DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25441_rabbit-dna-ligase-iii-mab-hl2280-azide-and-bsa-free-2120249422666.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25441_rabbit-dna-ligase-iii-mab-hl2280-azide-and-bsa-free-212024936275.jpg)
![Western Blot: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25441_rabbit-dna-ligase-iii-mab-hl2280-azide-and-bsa-free-212024941446.jpg)
![Western Blot: DNA Ligase III Antibody [NB100-81661] - Detection of Human DNL3 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody NB100-81661 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Western-Blot-NB100-81661-img0002.jpg "Western Blot: DNA Ligase III Antibody [NB100-81661] - Detection of Human DNL3 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody NB100-81661 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DNA Ligase III Antibody [NB100-81661] - Detection of human DNA Ligase III by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNA Ligase III antibody NB100-81661 used for IP at 6 ug per reaction. DNA Ligase III was also immunoprecipitated by rabbit anti-DNA Ligase III antibody from Company B. For blotting immunoprecipitated DNA Ligase III, NB100-81661 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunoprecipitation-NB100-81661-img0003.jpg)
![Western Blot: DNA Ligase III Overexpression Lysate (Adult Normal) [NBL1-12520] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase III.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12520-img0001.jpg "Western Blot: DNA Ligase III Overexpression Lysate (Adult Normal) [NBL1-12520] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNA Ligase III.")