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![Western Blot: FoxC1 Antibody [NB100-1268] - Left- Significant upregulation of Foxc1 mRNA in Ezh2-/- Tet-ON PyVmT endpoint tumours compared to wild-type tumours. Right- Immunoblot of Ezh2+/+ or Ezh2-/- Tet-ON PyVmT endpoint tumours for Foxc1 and Ezh2 levels. Vinculin loading control. *p<0.05, two tailed t-test. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04864-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Western-Blot-NB100-1268-img0018.jpg "<b>Biological Strategies Validation. </b>Western Blot: FoxC1 Antibody [NB100-1268] - Left- Significant upregulation of Foxc1 mRNA in Ezh2-/- Tet-ON PyVmT endpoint tumours compared to wild-type tumours. Right- Immunoblot of Ezh2+/+ or Ezh2-/- Tet-ON PyVmT endpoint tumours for Foxc1 and Ezh2 levels. Vinculin loading control. *p<0.05, two tailed t-test. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04864-8), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1268-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: FoxC1 Antibody [NB100-1268] - Representative images of formalin fixed paraffin-embedded tissue samples from human Luminal B tumours stained for FOXC1 and EZH2. Scale bar is 100um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04864-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunohistochemistry-NB100-1268-img0016.jpg)
![Flow Cytometry: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody at 2 ug/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Flow-Cytometry-NB100-1268-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: FoxC1 Antibody [NB100-1268] - Left: Representative immunoblot showing Foxc1 expression in cells stably transduced with a control shRNA (shLacZ) or two independent shRNAs targeting Foxc1 and treated with DMSO or GSK-126 (2uM for 72h). a-Tubulin was used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04864-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Knockdown-Validated-NB100-1268-img0017.jpg)
![Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody [NB100-1268] - Breast Cancer subtype specificity of the EZH2/FOXC1 metastatic axis. a Representative images of formalin fixed paraffin-embedded tissue samples from human Luminal B tumours stained for FOXC1 & EZH2. Scale bar is 100 μm. b Significant negative correlation between FOXC1 transcript levels & EZH2 protein levels (Total intensity) quantified by immunofluorescence in human Luminal B patient samples (r = −0.52, p = 0.02). Eight different fields taken with a ×20 objective were quantified per sample. c Kaplan–Meier survival curve of relapse-free survival (RFS) of 1142 luminal B tumours with high or low FOXC1 (log rank p = 0.001). d Correlation between FOXC1 mRNA levels & the FOXC1 Signature in a cohort of 385 Luminal B patients from publicly available TCGA Breast Cancer data set. e Kaplan–Meier analysis of relapse-free survival of 1142 Luminal B tumours with high or low FOXC1 gene signature expression (log rank p = 0.0044). RFS relapse-free survival Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29959321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1268_goat-polyclonal-foxc1-antibody-310202415541964.jpg)
![Western Blot: FoxC1 Antibody [NB100-1268] - Ectopic expression of Foxc1 alters invasion but not proliferation. a Transwell invasion assay of PyVmT cells expressing exogenous Foxc1 or GFP. Experiments were performed three times, & results are displayed relative to untreated parental cell. *p < 0.05, Student’s two tailed t-tests. b CyQUANT assay measuring cellular proliferation of PyVmT cells expressing exogenous GFP or Foxc1. R.F.U relative fluorescence units. Data were normalized to the fluorescence values at time = 0. c Enumeration of lung lesions following the injection of PyVmT cells expressing exogenous Foxc1 or GFP in the tail vein of athymic nude mice. *p < 0.05, Student’s two tailed t-tests. Scale bar is 5 mm. d Left: Representative immunoblot showing Foxc1 expression in cells stably transduced with a control shRNA (shLacZ) or two independent shRNAs targeting Foxc1 & treated with DMSO or GSK-126 (2 μM for 72 h). alpha -Tubulin was used as a loading control. Right: Transwell invasion assay of PyVmT cells infected with shRNA targeting LacZ (control) or two different sequences targeting Foxc1. Cells were pre-treated with DMSO or GSK-126 (2 μM) for 72 h & assayed for their capacity to invade through Matrigel. All assays were performed in triplicate. *p < 0.05, Student’s two tailed t-tests Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29959321), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1268_goat-polyclonal-foxc1-antibody-3102024168211.jpg)
![Immunohistochemistry-Paraffin: FoxC1 Antibody [NBP1-89025] - Staining in human salivary gland and liver tissues using anti-FOXC1 antibody. Corresponding FOXC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89025-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FoxC1 Antibody [NBP1-89025] - Staining in human salivary gland and liver tissues using anti-FOXC1 antibody. Corresponding FOXC1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NBP1-89025] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89025-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: FoxC1 Antibody [NBP1-89025] - Mouse embryonic brain section was stained with anti-FOXC1 antibody.Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89025-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxC1 Antibody [NBP1-89025] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89025-img0006.jpg)
![Immunohistochemistry-Paraffin: FoxC1 Antibody [NBP1-89025] - Staining of human salivary gland shows high expression.](http://images.novusbio.com/fullsize/FoxC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89025-img0007.jpg)
![Western Blot: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Western blot analysis of FoxC1 on different lysates with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: C2C12 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32347) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32347_rabbit-foxc1-mab-psh03-89-278202415212454.jpg "Western Blot: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Western blot analysis of FoxC1 on different lysates with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: C2C12 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32347) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32347) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32347_rabbit-foxc1-mab-psh03-89-29820241420930.jpg)
![Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody (PSH03-89) [NBP3-32347] - Immunocytochemistry analysis of HeLa cells labeling FoxC1 with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FoxC1 antibody (NBP3-32347) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32347_rabbit-foxc1-mab-psh03-89-2920241114841.jpg)
![Western Blot: FoxC1 Antibody [NBP3-05017] - Analysis of extracts of various cell lines, using FoxC1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FoxC1-Antibody-Western-Blot-NBP3-05017-img0001.jpg "Western Blot: FoxC1 Antibody [NBP3-05017] - Analysis of extracts of various cell lines, using FoxC1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunohistochemistry;FoxC1 Antibody[NBP3-29487]Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FOXC1 used at a dilution of 1:1,000 . Detection: DAB. Counterstain: hematoxylin .](http://images.novusbio.com/fullsize/nbp3-29488_rabbit-foxc1-pab-78202410153523.jpg "Immunohistochemistry;FoxC1 Antibody[NBP3-29487]Detection of human FOXC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FOXC1 used at a dilution of 1:1,000 . Detection: DAB. Counterstain: hematoxylin .")
![Western Blot: FOXC1 Overexpression Lysate (Adult Normal) [NBL1-10801] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXC1.](http://images.novusbio.com/fullsize/FOXC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10801-img0002.jpg "Western Blot: FOXC1 Overexpression Lysate (Adult Normal) [NBL1-10801] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXC1.")
![Western Blot: FoxC1 Antibody (SR2414) [NBP3-27713] - Western blot analysis of FoxC1 in 3T3 lysates](http://images.novusbio.com/fullsize/nbp3-27713_rabbit-foxc1-mab-sr2414-75202410133432.jpg "Western Blot: FoxC1 Antibody (SR2414) [NBP3-27713] - Western blot analysis of FoxC1 in 3T3 lysates")