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![Western Blot: eIF5A Antibody [NBP1-51982] - Analysis of eIF5A in MOLT4 lysate (35 ug protein in RIPA buffer) using NBP1-51982 at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa band according to NP_001137232.1).](http://images.novusbio.com/fullsize/eIF5A-Antibody-Western-Blot-NBP1-51982-img0002.jpg "Western Blot: eIF5A Antibody [NBP1-51982] - Analysis of eIF5A in MOLT4 lysate (35 ug protein in RIPA buffer) using NBP1-51982 at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa band according to NP_001137232.1).")
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP1-51982] - Staining of paraffin embedded Human Spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-51982-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP1-51982] - Staining of paraffin embedded Human Vessel. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-51982-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP1-51982] - Staining of paraffin embedded Human Tonsil. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP1-51982-img0004.jpg)
![Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] - Image of eIF5A Antibody (5E1) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-2722024831372.jpg "Immunohistochemistry: eIF5A Antibody (5E1) [NBP3-26604] - Image of eIF5A Antibody (5E1) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: eIF5A Antibody (5E1) [NBP3-26604] - Positive Western Blot detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate. All lanes: eIF5A Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 17, 21 kDa Observed band size: 18 kDa](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-28220241148586.jpg)
![Immunocytochemistry/Immunofluorescence: eIF5A Antibody (5E1) [NBP3-26604] - Staining of Hela Cells with eIF5A Antibody (5E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-28220241247518.jpg)
![Flow Cytometry: eIF5A Antibody (5E1) [NBP3-26604] - Overlay histogram showing Jurkat cells stained with eIF5A Antibody (5E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26604_rabbit-eif5a-mab-5e1-282202414183642.jpg)
![Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates](http://images.novusbio.com/fullsize/eIF5A-Antibody-4D1-Western-Blot-NBP2-59403-img0001.jpg "Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates")
![Immunocytochemistry/Immunofluorescence: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/eIF5A-Antibody-4D1-Immunocytochemistry-Immunofluorescence-NBP2-59403-img0002.jpg)
![Flow Cytometry: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)](http://images.novusbio.com/fullsize/eIF5A-Antibody-4D1-Flow-Cytometry-NBP2-59403-img0003.jpg)
![Western Blot: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.Lane 1: EIF5A transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/eIF5A-Antibody-8C1-Western-Blot-H00001984-M01-img0004.jpg "Western Blot: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.Lane 1: EIF5A transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of monoclonal antibody to EIF5A on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/eIF5A-Antibody-8C1-Immunocytochemistry-Immunofluorescence-H00001984-M01-img0002.jpg)
![Sandwich ELISA: eIF5A Antibody (8C1) [H00001984-M01] - Detection limit for recombinant GST tagged EIF5A is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/eIF5A-Antibody-8C1-Sandwich-ELISA-H00001984-M01-img0003.jpg)
![Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409] - Western blot analysis of extracts of various cell lines, using eIF5A Rabbit mAb (NBP3-16409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/eIF5A-Antibody-3R10I7-Western-Blot-NBP3-16409-img0001.jpg "Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409] - Western blot analysis of extracts of various cell lines, using eIF5A Rabbit mAb (NBP3-16409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: eIF5A Antibody [NBP2-38911] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/eIF5A-Antibody-Immunohistochemistry-Paraffin-NBP2-38911-img0002.jpg "Immunohistochemistry-Paraffin: eIF5A Antibody [NBP2-38911] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Western Blot: eIF5A Overexpression Lysate (Adult Normal) [NBL1-10214] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF5A.](http://images.novusbio.com/fullsize/eIF5A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10214-img0002.jpg "Western Blot: eIF5A Overexpression Lysate (Adult Normal) [NBL1-10214] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF5A.")
![Western Blot: eIF5A Overexpression Lysate (Adult Normal) [NBP2-09679] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF5A.](http://images.novusbio.com/fullsize/eIF5A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09679-img0001.jpg "Western Blot: eIF5A Overexpression Lysate (Adult Normal) [NBP2-09679] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF5A.")
![Western Blot: eIF5A Overexpression Lysate (Adult Normal) [NBP2-09680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF5A.](http://images.novusbio.com/fullsize/eIF5A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09680-img0001.jpg "Western Blot: eIF5A Overexpression Lysate (Adult Normal) [NBP2-09680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF5A.")
![SDS-Page: eIF5A Protein [NBC1-21053] - eIF5A, 16.8 kDa (154 aa) , confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/eIF5A-Protein-SDS-Page-NBC1-21053-img0001.jpg "SDS-Page: eIF5A Protein [NBC1-21053] - eIF5A, 16.8 kDa (154 aa) , confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")