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![Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining in human lymph node and pancreas tissues using anti-CRLF3 antibody. Corresponding CRLF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Immunohistochemistry-Paraffin-NBP1-84382-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining in human lymph node and pancreas tissues using anti-CRLF3 antibody. Corresponding CRLF3 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: CRLF3 Antibody [NBP1-84382] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-NBP1-84382-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CRLF3 Antibody [NBP1-84382] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84382-img0006.jpg)
![Western Blot: CRLF3 Antibody [NBP1-84382] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-NBP1-84382-img0005.jpg)
![Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Immunohistochemistry-Paraffin-NBP1-84382-img0007.jpg)
![Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Immunohistochemistry-Paraffin-NBP1-84382-img0008.jpg)
![Western Blot: CRLF3 Antibody [H00051379-B01P] - Analysis of CRLF3 expression in human spleen.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-H00051379-B01P-img0006.jpg "Western Blot: CRLF3 Antibody [H00051379-B01P] - Analysis of CRLF3 expression in human spleen.")
![Immunocytochemistry/Immunofluorescence: CRLF3 Antibody [H00051379-B01P] - Analysis of purified antibody to CRLF3 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/CRLF3-Antibody-Immunocytochemistry-Immunofluorescence-H00051379-B01P-img0004.jpg)
![Western Blot: CRLF3 Antibody [H00051379-B01P] - Analysis of CRLF3 expression in HeLa.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-H00051379-B01P-img0005.jpg)
![Western Blot: CRLF3 Antibody [H00051379-B01P] - Analysis of CRLF3 expression in transfected 293T cell line by CRLF3 polyclonal antibody. Lane 1: CRLF3 transfected lysate(48.62 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-H00051379-B01P-img0007.jpg)
![Western Blot: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Raji cell lysate (20 ug/Lane) Lane 3: Ramos cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: 293T cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse thymus tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat thymus tissue lysate (40 ug/Lane) Lane 13: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32223_rabbit-crlf3-mab-psh03-88-278202415212426.jpg "Western Blot: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Raji cell lysate (20 ug/Lane) Lane 3: Ramos cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: 293T cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse thymus tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat thymus tissue lysate (40 ug/Lane) Lane 13: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRLF3 Antibody (PSH03-88) [NBP3-32223] - Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (NBP3-32223) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32223_rabbit-crlf3-mab-psh03-88-29202410234273.jpg)
![Western Blot: CRLF3 Antibody [H00051379-D01P] - Analysis of CRLF3 expression in mouse liver.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-H00051379-D01P-img0001.jpg "Western Blot: CRLF3 Antibody [H00051379-D01P] - Analysis of CRLF3 expression in mouse liver.")
![Western Blot: CRLF3 Antibody [H00051379-D01P] - Analysis of CRLF3 expression in transfected 293T cell line by CRLF3 polyclonal antibody.Lane 1: CRLF3 transfected lysate(49.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CRLF3-Antibody-Western-Blot-H00051379-D01P-img0002.jpg)
![SDS-Page: CRLF3 Recombinant Protein [H00051379-P01]](http://images.novusbio.com/fullsize/CRLF3-Recombinant-Protein-SDS-Page-H00051379-P01-img0001.jpg "SDS-Page: CRLF3 Recombinant Protein [H00051379-P01]")
![Western Blot: CRLF3 Overexpression Lysate (Adult Normal) [NBL1-09486] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRLF3.](http://images.novusbio.com/fullsize/CRLF3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09486-img0002.jpg "Western Blot: CRLF3 Overexpression Lysate (Adult Normal) [NBL1-09486] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRLF3.")