Hu,
Mu,
RtSpecies Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, ICC/IF, KD, KO, Single-Cell Western
Applications: WB, Simple Western, ICC/IF, KD, KO, Single-Cell Western
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: CRBN Antibody [NBP1-91810] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP1-91810-img0034.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CRBN Antibody [NBP1-91810] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91810-img0027.jpg)
![Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP1-91810-img0025.jpg)
![Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CRBN-Antibody-Simple-Western-NBP1-91810-img0014.jpg)
![Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/CRBN-Antibody-Simple-Western-NBP1-91810-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Single Cell Western: CRBN Antibody [NBP1-91810] - CRBN expression after Downregulation by siRNA, dilution 1:500. Single Cell Western Image submitted by verified customer review.](http://images.novusbio.com/fullsize/CRBN-Antibody-Single-Cell-Western-NBP1-91810-img0035.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: CRBN Antibody [NBP1-91810] - Validation of SALL4 asa bona fide IMiD-dependent CRL4CRBN substrate. Parental Kelly cells or two independent pools of CRBN -/- Kelly cells were treated with increasing concentrations of thalidomide. Following 24 h incubation, SALL4, CRBN, and GAPDH protein levels were assessed by western blot analysis. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30067223/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP1-91810-img0036.jpg)
![Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202420243341.jpg "Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![Western Blot: CRBN Antibody [NBP2-82079] - Analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP2-82079-img0001.jpg)
![Immunohistochemistry: CRBN Antibody [NBP2-82079] - Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunohistochemistry-NBP2-82079-img0002.jpg)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Human Cells. Loading: 15 ug of cell lysate Antibodies: CRBN, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-271120242013238.gif)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Mouse Tissues.Loading: 15 ug of mouse tissue lysate Antibodies: CRBN, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202420141976.gif)
![Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Rat Tissues. Loading: 15 ug of rat tissue lysate Antibodies: CRBN, 0.5 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-82079_rabbit-polyclonal-crbn-antibody-2711202419592026.gif)
![Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP2-82079] - Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82079-img0003.jpg)
![Western Blot: CRBN Antibody [H00051185-B01P] - Analysis of CRBN expression in transfected 293T cell line by CRBN polyclonal antibody. Lane 1: CRBN transfected lysate(48.62 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-H00051185-B01P-img0002.jpg "Western Blot: CRBN Antibody [H00051185-B01P] - Analysis of CRBN expression in transfected 293T cell line by CRBN polyclonal antibody. Lane 1: CRBN transfected lysate(48.62 KDa). Lane 2: Non-transfected lysate.")
![SDS-Page: Recombinant Human CRBN Protein [H00051185-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-CRBN-Protein-SDS-Page-H00051185-P01-img0002.jpg "SDS-Page: Recombinant Human CRBN Protein [H00051185-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: CRBN Antibody (PSH0-70) [NBP3-32217] - Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (NBP3-32217) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32217) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32217_rabbit-crbn-mab-psh0-70-27820241628525.jpg "Western Blot: CRBN Antibody (PSH0-70) [NBP3-32217] - Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (NBP3-32217) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat testis tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32217) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRBN Antibody (PSH0-70) [NBP3-32217] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (NBP3-32217) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32217_rabbit-crbn-mab-psh0-70-298202413585562.jpg)
![Western Blot: CRBN Antibody [NBP2-92446] - Western blot analysis of extracts of various cell lines, using CRBN antibody (NBP2-92446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CRBN Antibody-Western Blot-NBP2-92446-img0001.jpg "Western Blot: CRBN Antibody [NBP2-92446] - Western blot analysis of extracts of various cell lines, using CRBN antibody (NBP2-92446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: CRBN Antibody [NBP2-87207] - Host: Rabbit. Target Name: CRBN. Sample Type: Jurkat Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP2-87207-img0001.jpg "Western Blot: CRBN Antibody [NBP2-87207] - Host: Rabbit. Target Name: CRBN. Sample Type: Jurkat Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: CRBN Overexpression Lysate (Adult Normal) [NBP2-07061] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRBN.](http://images.novusbio.com/fullsize/CRBN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07061-img0001.jpg "Western Blot: CRBN Overexpression Lysate (Adult Normal) [NBP2-07061] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRBN.")
![ELISA: Human CRBN - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31215] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31215_human-crbn-ready-to-use-elisa-kit-colorimetric-206202415322775.png "ELISA: Human CRBN - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31215] - Standard Curve Reference")
![ELISA: Human CRBN ELISA Kit (Colorimetric) [NBP3-27431] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27431_human-crbn-elisa-kit-colorimetric-11420241025105.png "ELISA: Human CRBN ELISA Kit (Colorimetric) [NBP3-27431] - Standard Curve Reference")