| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61715-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4147-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4147-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1543-0.025ml | |||
![Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Western-Blot-NB100-56150-img0001.jpg "Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: BNIP3 Antibody - Unpurified [NB100-56150] - BNIP3 Antibody [NB100-56150] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using BNIP3 antibody at 1:1000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Mitochondrial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NB100-56150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77683-img0005.jpg "Immunocytochemistry/Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: BNIP3 Antibody [NBP1-77683] - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-NBP1-77683-img0004.jpg)
![Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Flow-Cytometry-NBP1-77683-img0007.jpg)
![Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Flow-Cytometry-NBP1-77683-img0006.jpg)
![Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Western-Blot-NBP2-67192-img0009.jpg "Western Blot: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of BNIP3 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0008.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (JA71-10) [NBP2-67192] - Staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunocytochemistry-Immunofluorescence-NBP2-67192-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0002.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (JA71-10) [NBP2-67192] - Analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-JA71-10-Immunohistochemistry-Paraffin-NBP2-67192-img0004.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0006.jpg "Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against human BNIP3 (AA: 50-155) recombinant protein. (Expected MW is 38 kDa)")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunocytochemistry-Immunofluorescence-NBP2-61715-img0003.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of rectum cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0005.jpg)
![Flow Cytometry: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of Hela cells using BNIP3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Flow-Cytometry-NBP2-61715-img0002.jpg)
![Western Blot: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis using BNIP3 mAb against HEK293 (1) and BNIP3 (AA: 50-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Western-Blot-NBP2-61715-img0007.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody (6A5F7) [NBP2-61715] - Analysis of breast cancer tissues using BNIP3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-Immunohistochemistry-NBP2-61715-img0004.jpg)
![ELISA: BNIP3 Antibody (6A5F7) [NBP2-61715] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BNIP3-Antibody-6A5F7-ELISA-NBP2-61715-img0001.jpg)
![Western Blot: BNIP3 Antibody [NBP1-82566] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Western-Blot-NBP1-82566-img0006.jpg "Western Blot: BNIP3 Antibody [NBP1-82566] - Analysis in human cell line U-87 MG.")
![Immunohistochemistry-Paraffin: BNIP3 Antibody [NBP1-82566] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-82566-img0010.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody [NBP1-82566] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-82566-img0008.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody [NBP1-82566] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-82566-img0009.jpg)
![Immunohistochemistry-Paraffin: BNIP3 Antibody [NBP1-82566] - Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/BNIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-82566-img0011.jpg)
![Western Blot: BNIP3 Antibody (ANa40) [NB100-1543] - Monoclonal Anti-BNIP3 antibody produced in mouse](http://images.novusbio.com/fullsize/BNIP3-Antibody-ANa40-Western-Blot-NB100-1543-img0001.jpg "Western Blot: BNIP3 Antibody (ANa40) [NB100-1543] - Monoclonal Anti-BNIP3 antibody produced in mouse")
![Western Blot: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Western blot analysis of extracts of various cell lines, using BNIP3 antibody (NBP3-15799) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37C for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/BNIP3-Antibody-7Q7V3-Western-Blot-NBP3-15799-img0002.jpg "Western Blot: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Western blot analysis of extracts of various cell lines, using BNIP3 antibody (NBP3-15799) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37C for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: BNIP3 Antibody (7Q7V3) [NBP3-15799] - Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (NBP3-15799) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BNIP3-Antibody-7Q7V3-Immunohistochemistry-Paraffin-NBP3-15799-img0001.jpg)
![Western Blot: BNIP3 Antibody (S09-1G8) [NBP3-19550] - Western blot detection of BNIP3 in Rat Brain lysates using NBP3-19550 (1:500 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.](http://images.novusbio.com/fullsize/BNIP3-Antibody-S09-1G8-Western-Blot-NBP3-19550-img0002.jpg "Western Blot: BNIP3 Antibody (S09-1G8) [NBP3-19550] - Western blot detection of BNIP3 in Rat Brain lysates using NBP3-19550 (1:500 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.")
![Immunocytochemistry/Immunofluorescence: BNIP3 Antibody (S09-1G8) [NBP3-19550] - Immunofluorescence of BNIP3 (green) in Hela cells using NBP3-19550 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/BNIP3-Antibody-S09-1G8-Immunocytochemistry-Immunofluorescence-NBP3-19550-img0001.jpg)