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![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 uM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. Cells were scrapped into TBS containing protease inhibitors. Equal amounts of cell protein were applied into a 6% polyacrylamide electrophoresis gel and immunoblot was performed utilizing anti ABCG-1 antibody. Data (arbitrary units; n = 4) were corrected per beta-actin. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-172), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0016.jpg "Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - ABCG-1 protein level in control and glycolaldehyde-treated macrophages. J774 macrophages were overloaded with oxidized LDL (50 ug/mL de DMEM) for 48 h. In the last 5h, cells were incubated with 0.25 mM glycolaldehyde (GAD; black bars) and 1 uM LXR agonist (T0901317). Control cells (C; gray bars) were incubated with T0901317 alone. Cells were scrapped into TBS containing protease inhibitors. Equal amounts of cell protein were applied into a 6% polyacrylamide electrophoresis gel and immunoblot was performed utilizing anti ABCG-1 antibody. Data (arbitrary units; n = 4) were corrected per beta-actin. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/1476-511X-10-172), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: ABCG1 Antibody - BSA Free [NB400-132] - Staining of human lung, alveolar macrophages.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB400-132-img0013.jpg)
![Flow (Intracellular): ABCG1 Antibody - BSA Free [NB400-132] - RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours (B). An intracellular stain was performed with NB400-132 (blue) and a matched isotype control NB810-56910 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rabbit Dylight 550-conjugated antibody.](http://images.novusbio.com/fullsize/ABCG1-Antibody-BSA-Free-Flow-Intracellular-NB400-132-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Cells were pretreated with 5 uM F12511 or 5 uM MG132 for 30 min and then exposed to 5 or 50 uM 24S-OHC for 6 h. Whole-cell lysates were immunoblotted with antibodies specific for ABCG1 or beta-actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31285856/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCG1 Antibody - BSA Free-Western Blot-NB400-132-img0017.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0005.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0009.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Lane 1: untreated mouse peritoneal macrophages, Lane 2: 22-(R)-hydrocholesterol treated macrophages, Lane 3: T0901713 treated macrophages, Lane 4: T091713 treated macrophages.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0004.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - SAA has little impact on the impairment in biliary and fecal excretion of macrophage-derived 3H-cholesterol during endotoxemia. WT and SAAKO mice were injected subcutaneously with PBS or 0.8 ug/g LPS as indicated, followed 4 hr later by intraperitoneal injection of 3H-cholesterol-labeled J774 macrophages. ABCG1 in liver lysates (40 ug protein) was identified by immunoblotting and quantified by densitometry. Results from each of the 3 experiments (n = 4-5 per group per experiment) were expressed relative to PBS-injected WT mice after normalization to beta-actin. A representative western blot is shown. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jl/2013/283486/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCG1-Antibody---BSA-Free-Western-Blot-NB400-132-img0014.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - 6‐Dihydroparadol increases A) ABCA1 & B) ABCG1 protein stability. THP‐1 cells were differentiated as described in Figure 2. Then cells were loaded with unlabeled cholesterol & treated with 6‐dihydroparadol (10 μm), or solvent vehicle (0.1% DMSO, control) for 24 h. Cells were lysed at different time points (0, 1, 2, 3, 4, & 6 h) after treatment with the protein synthesis inhibitor cycloheximide (CHX, 100 μm). The protein levels of both ABCA1 & ABCG1 were detected by Western blot analysis. The data points represent mean ± SD from three independent experiments. *p < 0.05 & **p < 0.01 versus control at the same time point (determined by Student's t‐test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29802792), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-132_rabbit-polyclonal-abcg1-antibody-310202415291917.jpg)
![Western Blot: ABCG1 Antibody - BSA Free [NB400-132] - Evodiamine enhances apo A1-mediated ChE from THP-1 macrophages & increases ABCA1 protein level. (a) Differentiated THP-1 cells were loaded with [3H]-cholesterol together with the indicated treatments for 24 h. On the next day, the cells were washed twice with PBS & incubated with the same compounds [solvent vehicle control (Veh; ≤0.1% DMSO), evodiamine (1–20 μM), & the PPAR gamma agonist pioglitazone (10 μM) as positive control] with or without 10 µg/mL apo A1. Extracellular as well as intracellular radioactivities were quantified with scintillation counter. Differentiated THP-1-derived macrophages were treated with solvent vehicle control (Veh; ≤0.1% DMSO), evodiamine (10 μM), & the PPAR gamma agonist pioglitazone (10 μM) as positive control. After 24 h incubation, the cells were lysed & 20 μg protein was resolved via SDS-PAGE. Immunodetection was performed with antibodies against the indicated proteins, ABCA1 (b), ABCG1 (c), & SR-B1 (d), & visualized by chemiluminescence detection. All experiments were performed at least three times & data are presented as means ± S.D. vs. solvent vehicle control, *p < 0.05, **p < 0.01, ***p < 0.001, n.s. no significance (ANOVA/Bonferroni). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30038271), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-132_rabbit-polyclonal-abcg1-antibody-31020241618932.jpg)
![Western Blot: ABCG1 Antibody [NBP2-54682] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/ABCG1-Antibody-Western-Blot-NBP2-54682-img0003.jpg "Western Blot: ABCG1 Antibody [NBP2-54682] - Analysis in human cell line Daudi.")
![Immunohistochemistry-Paraffin: ABCG1 Antibody [NBP2-54682] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ABCG1-Antibody-Immunohistochemistry-Paraffin-NBP2-54682-img0001.jpg)
![Western Blot: ABCG1 Antibody (4Y7B3) [NBP3-15558] - Western blot analysis of extracts of various cell lines, using ABCG1 antibody (NBP3-15558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ABCG1-Antibody-4Y7B3-Western-Blot-NBP3-15558-img0001.jpg "Western Blot: ABCG1 Antibody (4Y7B3) [NBP3-15558] - Western blot analysis of extracts of various cell lines, using ABCG1 antibody (NBP3-15558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: ABCG1 Antibody (S06-5B9) [NBP3-20076] - Western blot detection of ABCG1 in Hela cell lysates using NBP3-20076 (1:1000 diluted). Predicted band size: 76kDa. Observed band size: 100kDa.](http://images.novusbio.com/fullsize/ABCG1-Antibody-S06-5B9-Western-Blot-NBP3-20076-img0001.jpg "Western Blot: ABCG1 Antibody (S06-5B9) [NBP3-20076] - Western blot detection of ABCG1 in Hela cell lysates using NBP3-20076 (1:1000 diluted). Predicted band size: 76kDa. Observed band size: 100kDa.")
![Immunocytochemistry/Immunofluorescence: ABCG1 Antibody (2H8) [H00009619-M03] - Analysis of monoclonal antibody to ABCG1 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/ABCG1-Antibody-2H8-Immunocytochemistry-Immunofluorescence-H00009619-M03-img0004.jpg "Immunocytochemistry/Immunofluorescence: ABCG1 Antibody (2H8) [H00009619-M03] - Analysis of monoclonal antibody to ABCG1 on HeLa cell. Antibody concentration 20 ug/ml")
![ELISA: ABCG1 Antibody (2H8) [H00009619-M03] - Detection limit for recombinant GST tagged ABCG1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ABCG1-Antibody-2H8-ELISA-H00009619-M03-img0005.jpg)
![Western Blot: ABCG1 Antibody [NBP3-05328] - Western blot analysis of extracts of HeLa cells, using ABCG1 antibody (NBP3-05328) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ABCG1-Antibody-Western-Blot-NBP3-05328-img0001.jpg "Western Blot: ABCG1 Antibody [NBP3-05328] - Western blot analysis of extracts of HeLa cells, using ABCG1 antibody (NBP3-05328) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: ABCG1 Antibody [NBP2-54681] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, the Golgi apparatus & vesicles.](http://images.novusbio.com/fullsize/ABCG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54681-img0006.jpg "Immunocytochemistry/Immunofluorescence: ABCG1 Antibody [NBP2-54681] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm, the Golgi apparatus & vesicles.")
![ELISA: Human ABCG1 ELISA Kit (Chemiluminescence) [NBP2-66378] - Samples were spiked with high concentrations of Human ABCG1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66378_human-abcg1-elisa-kit-chemiluminescence-13220241626149.jpg "ELISA: Human ABCG1 ELISA Kit (Chemiluminescence) [NBP2-66378] - Samples were spiked with high concentrations of Human ABCG1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ABCG1 ELISA Kit (Chemiluminescence) [NBP2-66378] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66378_human-abcg1-elisa-kit-chemiluminescence-4320249124851.jpg)
![Sandwich ELISA: ABCG1 Antibody (2E11) [H00009619-M01] - Detection limit for recombinant GST tagged ABCG1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ABCG1-Antibody-2E11-Sandwich-ELISA-H00009619-M01-img0002.jpg "Sandwich ELISA: ABCG1 Antibody (2E11) [H00009619-M01] - Detection limit for recombinant GST tagged ABCG1 is approximately 0.1ng/ml as a capture antibody.")