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Products Related to Gene-ID: 9423

24 Results for "9423"
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1109-N1

Recombinant Mouse Netrin-1 Protein

(41 Publications)
25 μg
1109-N1
1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.
Mu
Species Glossary
Reactivity: Mu
Binding Activity
Applications: Binding Activity
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free 1109-N1-025/CF
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1109-N1/CF

Recombinant Mouse Netrin-1 Protein, CF

(38 Publications)
25 μg
1109-N1/CF
1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.
Mu
Species Glossary
Reactivity: Mu
Binding Activity
Applications: Binding Activity
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AF1109

Netrin-1 Antibody [Unconjugated]

(17 Publications)
100 μg
AF1109
UNC5A and UNC5C induce caspase-3 activation through DAPK1/PR65b during UPR. (A) Quantification of netrin-1–receptor expression by qRT-PCR (mean + SEM, n = 3). (B–F) Identification of the implicated netrin-1 receptors. HepaRG cells were transfected with siRNAs and treated with DTT or not for 4 hours (mock). (B) Assessment of XBP1 mRNA splicing by RT-PCR. Representative result, n = 3. (C) Assessment of netrin-1 protein knockdown by immunoblotting. Representative result, n = 3. (D and E) Assessment of transcript knockdown efficiencies. Graphs indicate (D) UNC5A and (E) UNC5C mRNA levels in siRNA-treated cells in comparison with control siRNA-treated cells (mean + SEM; n = 3; Mann–Whitney test; P < .05). (F) Caspase-3 activation is reversed by UNC5A or UNC5C knockdown after UPR induction. Graph indicates caspase-3 activity ratio of DTT vs untreated cells for each condition (mean + SEM; n = 3; Mann–Whitney test; P < .05). (G–J) Identification of the downstream signaling pathway. HepaRG cells were transfected with siRNAs and treated with DTT or not for 4 hours (mock). (G) Assessment of XBP1 mRNA splicing by RT-PCR. Representative result, n = 3. (H) Evaluation of netrin-1, DAPK1, and PR65 beta depletion by immunoblotting. Representative result, n = 3. (I) Caspase-3 activation is reversed by DAPK1 or PR65 beta knockdown. Graph indicates the caspase-3 activity ratio for each condition (mean + SEM; n = 3; Mann–Whitney test; P < .05). (J) PP2A activity is increased by netrin-1 depletion and reversed by reduced expression of PR65 beta . Graph indicates PP2A activity ratio for each condition (mean + SEM; n = 3; Mann–Whitney test; P < .05). *,**, or *** refer to statistical analyses. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28174720), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mu
Species Glossary
Reactivity: Mu
WB, B/N
Applications: WB, B/N
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Biotin BAF1109
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1109-SP
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6419-N1

Recombinant Human Netrin-1 Protein

(18 Publications)
25 μg
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free 6419-N1-025/CF
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6419-N1/CF

Recombinant Human Netrin-1 Protein, CF

(17 Publications)
25 μg
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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MAB1109

Netrin-1 Antibody (158936) [Unconjugated]

(12 Publications)
100 μg
MAB1109
UNC5A and UNC5C induce caspase-3 activation through DAPK1/PR65b during UPR. (A) Quantification of netrin-1–receptor expression by qRT-PCR (mean + SEM, n = 3). (B–F) Identification of the implicated netrin-1 receptors. HepaRG cells were transfected with siRNAs and treated with DTT or not for 4 hours (mock). (B) Assessment of XBP1 mRNA splicing by RT-PCR. Representative result, n = 3. (C) Assessment of netrin-1 protein knockdown by immunoblotting. Representative result, n = 3. (D and E) Assessment of transcript knockdown efficiencies. Graphs indicate (D) UNC5A and (E) UNC5C mRNA levels in siRNA-treated cells in comparison with control siRNA-treated cells (mean + SEM; n = 3; Mann–Whitney test; P < .05). (F) Caspase-3 activation is reversed by UNC5A or UNC5C knockdown after UPR induction. Graph indicates caspase-3 activity ratio of DTT vs untreated cells for each condition (mean + SEM; n = 3; Mann–Whitney test; P < .05). (G–J) Identification of the downstream signaling pathway. HepaRG cells were transfected with siRNAs and treated with DTT or not for 4 hours (mock). (G) Assessment of XBP1 mRNA splicing by RT-PCR. Representative result, n = 3. (H) Evaluation of netrin-1, DAPK1, and PR65 beta depletion by immunoblotting. Representative result, n = 3. (I) Caspase-3 activation is reversed by DAPK1 or PR65 beta knockdown. Graph indicates the caspase-3 activity ratio for each condition (mean + SEM; n = 3; Mann–Whitney test; P < .05). (J) PP2A activity is increased by netrin-1 depletion and reversed by reduced expression of PR65 beta . Graph indicates PP2A activity ratio for each condition (mean + SEM; n = 3; Mann–Whitney test; P < .05). *,**, or *** refer to statistical analyses. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28174720), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mu
Species Glossary
Reactivity: Mu
WB
Applications: WB
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1109-SP
Carrier Free MAB1109-500
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AF6419

Netrin-1 Antibody [Unconjugated]

(8 Publications)
100 μg
AF6419
Western blot shows lysates of IMR-32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Netrin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6419) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Netrin-1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB
Applications: WB
Sheep Polyclonal
Host: Sheep Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF6419-SP
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NB100-1605

Netrin-1 Antibody

(8 Publications)
0.5 ml
NB100-1605
Immunocytochemistry/Immunofluorescence: Netrin-1 Antibody [NB100-1605] - Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (1:500 dilution).
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Chicken Polyclonal
Host: Chicken Polyclonal
Unconjugated  
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128-N1/CF

Recombinant Chicken Netrin-1 Protein, CF

(8 Publications)
25 μg
Ch
Species Glossary
Reactivity: Ch
Binding Activity
Applications: Binding Activity
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128-N1

Recombinant Chicken Netrin-1 Protein

(8 Publications)
25 μg
Ch
Species Glossary
Reactivity: Ch
Binding Activity
Applications: Binding Activity
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free 128-N1-025/CF
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MAB128

Netrin-1 Antibody (126518)

(2 Publications)
500 μg
Ch
Species Glossary
Reactivity: Ch
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB128-SP
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AF128

Netrin-1 Antibody

(2 Publications)
100 μg
Ch
Species Glossary
Reactivity: Ch
WB
Applications: WB
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF128
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF128-SP
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