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![Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Western-Blot-NBP2-42190-img0003.jpg "Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Lgi1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Lgi1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0005.jpg)
![Western Blot: Lgi1 Antibody [NBP1-77320] - HeLa cell lysate with LGI1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Western-Blot-NBP1-77320-img0003.jpg "Western Blot: Lgi1 Antibody [NBP1-77320] - HeLa cell lysate with LGI1 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody - BSA Free [NBP1-77320] - Lgi1 Antibody [NBP1-77320] - Human Brain cells with LGI1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Immunofluorescence-NBP1-77320-img0004.jpg)
![Immunohistochemistry-Paraffin: Lgi1 Antibody [NBP1-77320] - Human brain tissue using LGI1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Immunohistochemistry-Paraffin-NBP1-77320-img0002.jpg)
![Western Blot: Lgi1 Antibody [NBP1-33087] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Western-Blot-NBP1-33087-img0004.jpg "Western Blot: Lgi1 Antibody [NBP1-33087] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody [NBP1-33087] - LGI1 antibody [N2C2], Internal detects LGI1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: LGI1 protein stained by LGI1 antibody [N2C2], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33087-img0005.jpg)
![Western Blot: Lgi1 Antibody [NBP1-33087] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Western-Blot-NBP1-33087-img0002.jpg)
![Western Blot: Lgi1 Antibody [NBP1-33087] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Western-Blot-NBP1-33087-img0003.jpg)
![Western Blot: Lgi1 Antibody (SR2092) [NBP3-22331] - Western blot analysis using NBP3-22331 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22331_rabbit-lgi1-mab-sr2092-5720231754882.jpg "Western Blot: Lgi1 Antibody (SR2092) [NBP3-22331] - Western blot analysis using NBP3-22331 in HeLa cell lysate.")
![Western Blot: Lgi1 Antibody [NBP1-76379] - Mouse brain tissue lysate with LGI1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Western-Blot-NBP1-76379-img0002.jpg "Western Blot: Lgi1 Antibody [NBP1-76379] - Mouse brain tissue lysate with LGI1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Western Blot: Lgi1 Antibody (2P8Z9) [NBP3-15694] - Western blot analysis of extracts of various cell lines, using Lgi1 antibody (NBP3-15694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Lgi1-Antibody-2P8Z9-Western-Blot-NBP3-15694-img0001.jpg "Western Blot: Lgi1 Antibody (2P8Z9) [NBP3-15694] - Western blot analysis of extracts of various cell lines, using Lgi1 antibody (NBP3-15694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Lgi1 Overexpression Lysate (Adult Normal) [NBL1-12506] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lgi1.](http://images.novusbio.com/fullsize/Lgi1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12506-img0002.jpg "Western Blot: Lgi1 Overexpression Lysate (Adult Normal) [NBL1-12506] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lgi1.")
![Western Blot: Lgi1 Antibody [H00009211-B01P] - Analysis of LGI1 expression in transfected 293T cell line by LGI1 polyclonal antibody. Lane 1: LGI1 transfected lysate(61.27 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Lgi1-Antibody-Western-Blot-H00009211-B01P-img0001.jpg "Western Blot: Lgi1 Antibody [H00009211-B01P] - Analysis of LGI1 expression in transfected 293T cell line by LGI1 polyclonal antibody. Lane 1: LGI1 transfected lysate(61.27 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Lgi1 Peptide [NBP1-76379PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.](http://images.novusbio.com/fullsize/Lgi1-Peptide-Western-Blot-NBP1-76379PEP-img0001.jpg "Western Blot: Lgi1 Peptide [NBP1-76379PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.")