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![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0013.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31378-img0006.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - NCI-N87 xenograft, using AKR1C3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NBP1-31378-img0007.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0008.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0010.jpg)
![Immunoprecipitation: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 ug of AKR1C3 antibody [C2C3], C-term. Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunoprecipitation-NBP1-31378-img0012.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-57202317215160.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - Western blot analysis using NBP3-22033 in A549 cell lysate.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: MCF-7. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462214.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: SH-SY5Y. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-2342024155511.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Hep-G2. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462212.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Mouse lung. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202415232515.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (SR1612) [NBP3-22033] - All Lanes: NBP3-22033 at 1/ 5000 dilution. Lane1: Rat kidney. Proteins at 20ug per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 37kDa. Observed band size: 37kDa](http://images.novusbio.com/fullsize/nbp3-22033_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-sr1612-234202414462210.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NB100-1940-img0002.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of human breast cells lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NB100-1940] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL.Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NB100-1940-img0003.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-33556-img0006.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NBP1-33556-img0004.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-33556-img0005.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Western blot analysis of extracts of various cell lines, using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb (NBP3-16288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-7T10F5-Western-Blot-NBP3-16288-img0002.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Western blot analysis of extracts of various cell lines, using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb (NBP3-16288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Confocal imaging of A549 cells using AKR1C3 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16288_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-7t10f5-immunocytochemistry-immunofluorescence-186202491659..jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (4D12) [NBP3-15212] - Immunofluorescence staining of Hela Cells with at Aldo-keto Reductase 1C3/AKR1C3 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-4D12-Immunocytochemistry-Immunofluorescence-NBP3-15212-img0002.jpg "Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (4D12) [NBP3-15212] - Immunofluorescence staining of Hela Cells with at Aldo-keto Reductase 1C3/AKR1C3 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Aldo-keto Reductase 1C3/AKR1C3 Antibody (4D12) [NBP3-15212] - Overlay histogram showing A549 cells stained with Aldo-keto Reductase 1C3/AKR1C3 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-4D12-Flow-Cytometry-NBP3-15212-img0001.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28) [NBP3-32015] - Western blot analysis of Aldo-keto Reductase 1C3/AKR1C3 on different lysates with Rabbit anti-Aldo-keto Reductase 1C3/AKR1C3 antibody (NBP3-32015) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Human kidney tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32015) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32015_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-je36-28-27820241514172.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28) [NBP3-32015] - Western blot analysis of Aldo-keto Reductase 1C3/AKR1C3 on different lysates with Rabbit anti-Aldo-keto Reductase 1C3/AKR1C3 antibody (NBP3-32015) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Human kidney tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32015) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Overexpression Lysate [NBP2-07959] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1C3.](http://images.novusbio.com/fullsize/AKR1C3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07959-img0001.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Overexpression Lysate [NBP2-07959] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1C3.")
