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![Western Blot: ULK1 Antibody [NBP2-41217] - Analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ULK1-Antibody-Western-Blot-NBP2-41217-img0001.jpg "Western Blot: ULK1 Antibody [NBP2-41217] - Analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry-Paraffin: ULK1 Antibody [NBP2-41217] - Rat brain tissue with ULK1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/ULK1-Antibody-Immunohistochemistry-NBP2-41217-img0002.jpg)
![Immunofluorescence: ULK1 Antibody [NBP2-41217] - ULK1 in rat brain tissue with ULK1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/ULK1-Antibody-Immunofluorescence-NBP2-41217-img0004.jpg)
![Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Western-Blot-NBP2-66765-img0005.jpg "Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate")
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0004.jpg)
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human colon cancer tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0001.jpg)
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0002.jpg)
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded mouse brain tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0003.jpg)
![Western Blot: ULK1 Antibody [NBP2-94753] - Western blot analysis of extracts of various cell lines, using ULK1 antibody (NBP2-94753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ULK1-Antibody-Western-Blot-NBP2-94753-img0003.jpg "Western Blot: ULK1 Antibody [NBP2-94753] - Western blot analysis of extracts of various cell lines, using ULK1 antibody (NBP2-94753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: ULK1 Antibody [NBP2-94753] - Analysis of L929 cells using ULK1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ULK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94753-img0001.jpg)
![Western Blot: ULK1 Antibody [NBP2-94753] - Western blot analysis of extracts of Rat brain, using ULK1 antibody (NBP2-94753) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ULK1-Antibody-Western-Blot-NBP2-94753-img0002.jpg)
![Western Blot: ULK1 Antibody [NBP2-94753] - Western blot analysis of extracts of Mouse brain, using ULK1 antibody (NBP2-94753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/ULK1 Antibody-Western Blot-NBP2-94753-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ULK1 Antibody [NBP2-56576] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ULK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56576-img0001.jpg "Immunocytochemistry/Immunofluorescence: ULK1 Antibody [NBP2-56576] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.")
![Western Blot: ULK1 [p Ser467] Antibody [NBP3-16957] - Western blot analysis of extracts of various cell lines, using ULK1 antibody (NBP3-16957) at 1:1000 dilution.HeLa and Jurkat cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ULK1-[p-Ser467]-Antibody-Western-Blot-NBP3-16957-img0001.jpg "Western Blot: ULK1 [p Ser467] Antibody [NBP3-16957] - Western blot analysis of extracts of various cell lines, using ULK1 antibody (NBP3-16957) at 1:1000 dilution.HeLa and Jurkat cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: ULK1 [p Ser 556] Antibody (SR1119) [NBP3-22612] - Western blot analysis using NBP3-22612 in 293T transfected with ULK1 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22612_rabbit-ulk1-p-ser-556-mab-sr1119-57202315521847.jpg "Western Blot: ULK1 [p Ser 556] Antibody (SR1119) [NBP3-22612] - Western blot analysis using NBP3-22612 in 293T transfected with ULK1 cell lysate.")
![Western Blot: ULK1 [p Ser556] Antibody (JE59-28) [NBP3-32814] - Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (NBP3-32814) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: MCF7 treated with pp for 1 hour whole cell lysate Lane 4: MCF7 treated with 0.5uM Oligomycin for 30 minutes, then treated with pp for 1 hour whole cell lysate Lysates/proteins at 14 ug/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32814) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32814_rabbit-ulk1-p-ser556-mab-je59-28-288202412461674.jpg "Western Blot: ULK1 [p Ser556] Antibody (JE59-28) [NBP3-32814] - Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (NBP3-32814) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 0.5uM Oligomycin for 30 minutes whole cell lysate Lane 3: MCF7 treated with pp for 1 hour whole cell lysate Lane 4: MCF7 treated with 0.5uM Oligomycin for 30 minutes, then treated with pp for 1 hour whole cell lysate Lysates/proteins at 14 ug/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32814) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ULK1 Antibody (RM488) [NBP3-23466] - Western Blot of the lysates of A431 cells nontreated (-) or treated (+) with Calyculin A/Okadaic Acid, using Clone RM488) at a 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-23466_rabbit-ulk1-mab-24620241332441.png "Western Blot: ULK1 Antibody (RM488) [NBP3-23466] - Western Blot of the lysates of A431 cells nontreated (-) or treated (+) with Calyculin A/Okadaic Acid, using Clone RM488) at a 1:1000 dilution.")
![Western Blot: ULK1 [p Ser638] Antibody [NBP3-13254] - Untreated (-) and treated (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser638) antibody (NBP3-13254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ULK1-[p-Ser638]-Antibody-Western-Blot-NBP3-13254-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ULK1 [p Ser638] Antibody [NBP3-13254] - Untreated (-) and treated (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser638) antibody (NBP3-13254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ULK1 [p Ser555] Antibody [NBP3-13124] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser555) antibody (NBP3-13124) diluted at 1:1000.](http://images.novusbio.com/fullsize/ULK1-[p-Ser555]-Antibody-Western-Blot-NBP3-13124-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ULK1 [p Ser555] Antibody [NBP3-13124] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser555) antibody (NBP3-13124) diluted at 1:1000.")
![Western Blot: ULK1 [p Ser758] Antibody [NBP3-13123] - Untreated (-) and treated (+) MCF7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser758) antibody (NBP3-13123) diluted at 1:500.](http://images.novusbio.com/fullsize/ULK1-[p-Ser758]-Antibody-Western-Blot-NBP3-13123-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ULK1 [p Ser758] Antibody [NBP3-13123] - Untreated (-) and treated (+) MCF7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser758) antibody (NBP3-13123) diluted at 1:500.")
![Western Blot: ULK1 Overexpression Lysate (Adult Normal) [NBL1-17607] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ULK1.](http://images.novusbio.com/fullsize/ULK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17607-img0002.jpg "Western Blot: ULK1 Overexpression Lysate (Adult Normal) [NBL1-17607] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ULK1.")