![Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Western-Blot-NBP2-66765-img0005.jpg "Western Blot: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of ULK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: MCF-7 cell lysate Lane 3: mouse spleen tissue lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | JA58-36 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human ULK1 aa 930-979 / 1050. (SwissProt: O75385 Human; SwissProt: O70405 Mouse; SwissProt: D3ZMG0 Rat) |
| Localization | Cytosol, Preautophagosomal structure. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ULK1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunohistochemistry ATG7 Antibody (683906) [Unconjugated]](https://images.novusbio.com/images2/ATG7_MAB6608_Immunohistochemistry_10631.jpg)
![Simple Western ATG7 Antibody (683906) [Unconjugated]](https://images.novusbio.com/images2/ATG7_MAB6608_Simple_Western_16409.jpg)
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Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ULK1 |
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0004.jpg "Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human colon cancer tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0001.jpg "Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human colon cancer tissue using anti-ULK1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0002.jpg "Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded mouse brain tissue using anti-ULK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ULK1-Antibody-JA58-36-Immunohistochemistry-Paraffin-NBP2-66765-img0003.jpg "Immunohistochemistry-Paraffin: ULK1 Antibody (JA58-36) [NBP2-66765] - Analysis of paraffin-embedded mouse brain tissue using anti-ULK1 antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")