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![Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NB200-210-img0012.jpg "Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: p400 Antibody [NB200-210] - Detection of human p400 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NB200-210-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p400 Antibody [NB200-210] - Detection of human p400 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p400 antibody NB200-210 (lot 2) used for IP at 6 ug per reaction. p400 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-p400 antibody from Company B. For blotting immunoprecipitated p400, NB200-210 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/p400-Antibody-Immunoprecipitation-NB200-210-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP1-88230] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p400-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88230-img0005.jpg "Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP1-88230] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/p400-Antibody-Immunohistochemistry-Paraffin-NBP1-88230-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230] - Analysis in human testis and liver tissues. Corresponding EP400 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p400-Antibody-Immunohistochemistry-Paraffin-NBP1-88230-img0006.jpg)
![Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/p400-Antibody-Immunohistochemistry-Paraffin-NBP1-88230-img0007.jpg)
![Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230] - Staining of human liver shows no nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/p400-Antibody-Immunohistochemistry-Paraffin-NBP1-88230-img0008.jpg)
![Immunohistochemistry-Paraffin: p400 Antibody [NBP1-88230] - Staining of human skin shows moderate nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/p400-Antibody-Immunohistochemistry-Paraffin-NBP1-88230-img0009.jpg)
![Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NBP2-19663-img0001.jpg "Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP2-19663] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/p400-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19663-img0002.jpg)
![Western Blot: p400 Antibody [H00057634-B01P] - Analysis of EP400 expression in transfected 293T cell line by EP400 polyclonal antibody. Lane 1: EP400 transfected lysate(108.35 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-H00057634-B01P-img0004.jpg "Western Blot: p400 Antibody [H00057634-B01P] - Analysis of EP400 expression in transfected 293T cell line by EP400 polyclonal antibody. Lane 1: EP400 transfected lysate(108.35 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: p400 Antibody [H00057634-B01P] - Analysis of purified antibody to EP400 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/p400-Antibody-Immunocytochemistry-Immunofluorescence-H00057634-B01P-img0003.jpg)
![Western Blot: p400 Antibody (2A7) [H00057634-M01] - Analysis of EP400 expression in transfected 293T cell line by EP400 monoclonal antibody (M01), clone 2A7.Lane 1: EP400 transfected lysate(105.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p400-Antibody-2A7-Western-Blot-H00057634-M01-img0001.jpg "Western Blot: p400 Antibody (2A7) [H00057634-M01] - Analysis of EP400 expression in transfected 293T cell line by EP400 monoclonal antibody (M01), clone 2A7.Lane 1: EP400 transfected lysate(105.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP2-55019] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/p400-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55019-img0001.jpg "Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP2-55019] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")