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![Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Western-Blot-NBP2-12906-img0008.jpg "Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunocytochemistry-Immunofluorescence-NBP2-12906-img0011.jpg)
![Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunohistochemistry-NBP2-12906-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Human hippocampal tissues.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunocytochemistry-Immunofluorescence-NBP2-12906-img0004.jpg)
![Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.](http://images.novusbio.com/fullsize/TRPM7-Antibody-S74-Immunohistochemistry-NBP2-12906-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - IF on PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP2-58525] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58525-img0008.jpg "Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP2-58525] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TRPM7 Antibody [NBP2-58525] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunohistochemistry-Paraffin-NBP2-58525-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TRPM7 Antibody [NBP2-58525] - Analysis in human parathyroid gland and pancreas tissues using Anti-TRPM7 antibody. Corresponding TRPM7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunohistochemistry-Paraffin-NBP2-58525-img0004.jpg)
![Immunohistochemistry-Paraffin: TRPM7 Antibody [NBP2-58525] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunohistochemistry-Paraffin-NBP2-58525-img0005.jpg)
![Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Western-Blot-NBP1-46829-img0002.jpg "Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRPM7 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TRPM7 was also immunoprecipitated by rabbit anti-TRPM7 antibody BL9785, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 minute (B).](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunoprecipitation-NBP1-46829-img0001.jpg)
![Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Detection of human TRPM7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRPM7 antibody NBP1-46829 (lot NBP1-46829-3) used for IP at 6 ug per reaction. TRPM7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46829-2). For blotting immunoprecipitated TRPM7, NBP1-46829 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunoprecipitation-NBP1-46829-img0003.jpg)
![Immunohistochemistry-Paraffin: TRPM7 Antibody [NB100-810] - (4ug/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunohistochemistry-Paraffin-NB100-810-img0004.jpg "Immunohistochemistry-Paraffin: TRPM7 Antibody [NB100-810] - (4ug/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.")
![Western Blot: TRPM7 Antibody (SR1322) [NBP3-21852] - Western blot analysis using NBP3-21852 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21852_rabbit-trpm7-mab-sr1322-57202315123411.jpg "Western Blot: TRPM7 Antibody (SR1322) [NBP3-21852] - Western blot analysis using NBP3-21852 in HeLa cell lysate.")
![Western Blot: TRPM7 Antibody (JE38-36) [NBP3-33061] - Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (NBP3-33061) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 213 kDa Observed band size: 250 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33061) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33061_rabbit-trpm7-mab-je38-36-288202412493421.jpg "Western Blot: TRPM7 Antibody (JE38-36) [NBP3-33061] - Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (NBP3-33061) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 213 kDa Observed band size: 250 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33061) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: TRPM7 Antibody (JE38-36) [NBP3-33061] - Flow cytometric analysis of HeLa cells labeling TRPM7. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33061, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33061_rabbit-trpm7-mab-je38-36-29202416453239.jpg)
![Western Blot: TRPM7 Antibody [NBP3-23361] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23361_rabbit-trpm7-pab-119202316331624.jpg "Western Blot: TRPM7 Antibody [NBP3-23361] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).")
![Western Blot: TRPM7 Antibody (M573) [NBP3-23439] - Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.](http://images.novusbio.com/fullsize/nbp3-23439_mouse-trpm7-mab-m573-11920231633167.jpg "Western Blot: TRPM7 Antibody (M573) [NBP3-23439] - Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.")
![Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23186_rabbit-trpm7-p-ser1513-pab-199202312523634.jpg "Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).")