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![Western Blot: PCK2 Antibody [NBP2-33606] - Analysis using Anti-PCK2 antibody NBP2-33606 (A) shows similar pattern to independent antibody NBP2-33685 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-33606-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PCK2 Antibody [NBP2-33606] - Analysis using Anti-PCK2 antibody NBP2-33606 (A) shows similar pattern to independent antibody NBP2-33685 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP2-33606] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33606-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human liver.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0015.jpg)
![Immunohistochemistry: PCK2 Antibody [NBP2-33606] - Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-NBP2-33606-img0007.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining in human duodenum and pancreas tissues using anti-PCK2 antibody. Corresponding PCK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human cerebral cortex, colon, kidney and liver using Anti-PCK2 antibody NBP2-33606 (A) shows similar protein distribution across tissues to independent antibody NBP2-33685 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0010.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human colon.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0012.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0013.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33606] - Staining of human kidney.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33606-img0014.jpg)
![Western Blot: PCK2 Antibody [NBP2-33685] - Analysis using Anti-PCK2 antibody NBP2-33685 (A) shows similar pattern to independent antibody NBP2-33606 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-33685-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PCK2 Antibody [NBP2-33685] - Analysis using Anti-PCK2 antibody NBP2-33685 (A) shows similar pattern to independent antibody NBP2-33606 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP2-33685] - Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33685-img0003.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human liver.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0012.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0004.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining in human duodenum and skeletal muscle tissues using anti-PCK2 antibody. Corresponding PCK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human cerebral cortex, colon, kidney and liver using Anti-PCK2 antibody NBP2-33685 (A) shows similar protein distribution across tissues to independent antibody NBP2-33606 (B).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0007.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human colon.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0009.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0010.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-33685] - Staining of human kidney.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33685-img0011.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0002.jpg "Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NB100-2422] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2422-img0003.jpg)
![Flow Cytometry: PCK2 Antibody [NB100-2422] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Flow-Cytometry-NB100-2422-img0004.jpg)
![Western Blot: PCK2 Antibody [NB100-2422] - Staining of A431 (A), HEK293 (B) with antibody at 2 ug/mL and HepG2 (C) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NB100-2422-img0001.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0005.jpg "Western Blot: PCK2 Antibody [NBP1-31241] - PCK2 antibody, Internal detects PCK2 protein. A. 30 ug HepG2 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP1-31241] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31241-img0006.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP1-31241] - OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-31241-img0002.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0003.jpg)
![Western Blot: PCK2 Antibody [NBP1-31241] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP1-31241-img0004.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0006.jpg "Western Blot: PCK2 Antibody [NBP2-19729] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NBP2-19729] - HepG2 cells were fixed in MeOH for 5 min. Green: PCK2 protein stained by PCK2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19729-img0007.jpg)
![Immunohistochemistry-Paraffin: PCK2 Antibody [NBP2-19729] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-19729-img0004.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - PCK2 antibody detects PCK2 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract7. 5 % SDS-PAGEPCK2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0001.jpg)
![Western Blot: PCK2 Antibody [NBP2-19729] - PCK2 antibody detects PCK2 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract7. 5 % SDS-PAGEPCK2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PCK2-Antibody-Western-Blot-NBP2-19729-img0002.jpg)
![Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of extracts of various cell lines, using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PCK2-Antibody-10L10J8-Western-Blot-NBP3-16418-img0002.jpg "Western Blot: PCK2 Antibody (10L10J8) [NBP3-16418] - Western blot analysis of extracts of various cell lines, using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PCK2 Antibody (10L10J8) [NBP3-16418] - Immunofluorescence analysis of HeLa cells using PEPCK/PCK2 Rabbit mAb (NBP3-16418) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PCK2-Antibody-10L10J8-Immunocytochemistry-Immunofluorescence-NBP3-16418-img0001.jpg)
![Western Blot: PCK2 Antibody (2C9) [H00005106-M05] - Analysis of PCK2 expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/PCK2-Antibody-2C9-Western-Blot-H00005106-M05-img0003.jpg "Western Blot: PCK2 Antibody (2C9) [H00005106-M05] - Analysis of PCK2 expression in A-431 (Cat # L015V1).")
![Sandwich ELISA: PCK2 Antibody (2C9) [H00005106-M05] - Detection limit for recombinant GST tagged PCK2 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PCK2-Antibody-2C9-Sandwich-ELISA-H00005106-M05-img0002.jpg)
![Western Blot: PCK2 Overexpression Lysate (Adult Normal) [NBL1-14180] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PCK2.](http://images.novusbio.com/fullsize/PCK2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14180-img0002.jpg "Western Blot: PCK2 Overexpression Lysate (Adult Normal) [NBL1-14180] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PCK2.")