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![Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-NBP1-46557-img0002.jpg "Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.")
![Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is highly expressed in human tissue grafts originating from both fetal and hESC. Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum with the phenotypic staining for TH , and the identification of human NCAM, imaged using LSM. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-NBP1-46557-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is Highly Expressed in Human Tissue Grafts Originating from Both Fetal & hESC Sources(A–E) Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum (A & C) with the phenotypic staining for TH (A & B), & the identification of human NCAM (A & D), imaged using LSM. The 5-HT6 expression was confirmed to reside in DA neurons originating from the transplanted tissue (E).(F–J) Similarly, immunofluorescence staining of a paraffin-embedded section originating from the putamen of a PD patient that received a dopaminergic fetal graft, imaged using LSM, confirmed that the 5-HT6 receptor (F & H) is highly expressed in the TH-positive (F & G, with overlay in J) DA neurons originating from the fetal graft. Nuclear staining (DAPI) confirmed the 5-HT6 expression to be abundant in the neuronal soma (I).(K–O) To confirm if this receptor is also abundant in DA neurons differentiated from hESCs, a graft derived from the H9 hESC line, differentiated using the novel floor-plate-based protocol & transplanted to the parkinsonian striatum of a nude rat, was evaluated using LSM with immunofluorescence for the same genes: 5-HT6 (K & M), TH (K & L), & the human NCAM (K & N). This confirmed that this receptor is highly expressed in DA neurons also differentiated from this cell source, when transplanted to the striatum. Overlay of all three antigens confirmed the 5-HT6 receptor to reside in hESC-derived DA neurons (O). Scale bar, 100 μm (K) & 50 μm (N). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27161524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-46557_rabbit-polyclonal-5-ht6-antibody-310202416171411.jpg)
![Immunohistochemistry-Paraffin: 5-HT6 Antibody [NLS652] - Analysis of anti-5HT6 antibody with human brain, cerebellum.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-Paraffin-NLS652-img0001.jpg "Immunohistochemistry-Paraffin: 5-HT6 Antibody [NLS652] - Analysis of anti-5HT6 antibody with human brain, cerebellum.")
![Immunohistochemistry-Paraffin: 5-HT6 Antibody - BSA Free [NLS652] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human, Brain, Cerebellum tissue](http://images.novusbio.com/fullsize/nls652_rabbit-5-ht6-pab-bsa-free-1092024110317.jpg)
![Immunohistochemistry-Paraffin: 5-HT6 Antibody [NBP3-17679] - Staining of human caudate shows moderate positivity in neuronal cells.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-Paraffin-NBP3-17679-img0001.jpg "Immunohistochemistry-Paraffin: 5-HT6 Antibody [NBP3-17679] - Staining of human caudate shows moderate positivity in neuronal cells.")
![Western Blot: 5-HT6 Antibody [NBP3-10802] - Western blot analysis of 5-HT6 in Human Jurkat Whole Cell lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/5-HT6-Antibody-Western-Blot-NBP3-10802-img0001.jpg "Western Blot: 5-HT6 Antibody [NBP3-10802] - Western blot analysis of 5-HT6 in Human Jurkat Whole Cell lysates. Antibody dilution at 1ug/ml")
![Western Blot: 5-HT6 Antibody [NBP3-10817] - Western blot analysis of 5-HT6 in Human COLO205 Whole Cell lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/5-HT6-Antibody-Western-Blot-NBP3-10817-img0001.jpg "Western Blot: 5-HT6 Antibody [NBP3-10817] - Western blot analysis of 5-HT6 in Human COLO205 Whole Cell lysates. Antibody dilution at 1ug/ml")
![Western Blot: 5HT6 Receptor Overexpression Lysate (Adult Normal) [NBL1-11782] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 5HT6 Receptor.](http://images.novusbio.com/fullsize/5HT6-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11782-img0002.jpg "Western Blot: 5HT6 Receptor Overexpression Lysate (Adult Normal) [NBL1-11782] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 5HT6 Receptor.")
![Immunoprecipitation: 5-HT6 Antibody [NBP2-32079] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-5-HT6 antibody NBP2-32079 used for IP at 6 ug per reaction. 5-HT6 was also immunoprecipitated by rabbit anti-5-HT6 antibody BL16176. For blotting immunoprecipitated 5-HT6, NBP2-32079 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunoprecipitation-NBP2-32079-img0002.jpg "Immunoprecipitation: 5-HT6 Antibody [NBP2-32079] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-5-HT6 antibody NBP2-32079 used for IP at 6 ug per reaction. 5-HT6 was also immunoprecipitated by rabbit anti-5-HT6 antibody BL16176. For blotting immunoprecipitated 5-HT6, NBP2-32079 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")