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![Knockdown Validated: Fer Antibody [NBP1-20089] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fer knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Fer Polyclonal Antibody (Catalog # NBP1-20089) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fer at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Fer-Antibody-Knockdown-Validated-NBP1-20089-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Fer Antibody [NBP1-20089] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fer knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Fer Polyclonal Antibody (Catalog # NBP1-20089) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fer at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FER Antibody [NBP1-20089] - Jurkat cell lysates.](http://images.novusbio.com/fullsize/FER-Antibody-Western-Blot-NBP1-20089-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FER Antibody [NBP1-20089] - FER antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/FER-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20089-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Fer Antibody [NBP1-86894] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fer-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86894-img0006.jpg "Immunocytochemistry/Immunofluorescence: Fer Antibody [NBP1-86894] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Fer Antibody [NBP1-86894] - Staining of human tonsil shows moderate nuclear positivity in lymphoid cells outside reaction center.](http://images.novusbio.com/fullsize/Fer-Antibody-Immunohistochemistry-Paraffin-NBP1-86894-img0001.jpg)
![Western Blot: Fer Antibody [NBP3-04827] - Analysis of extracts of various cell lines, using Fer antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/Fer-Antibody-Western-Blot-NBP3-04827-img0001.jpg "Western Blot: Fer Antibody [NBP3-04827] - Analysis of extracts of various cell lines, using Fer antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")