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MDM2/HDM2 Antibody

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 (2 Reviews)
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Summary
Product Discontinued
View other related MDM2/HDM2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-02158
    • Availability
      Product Discontinued

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MDM2/HDM2 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human MDM2 (within residues 300-400). [Swiss-Prot# Q00987]
Localization
Nucleus: nucleoplasm. Cytoplasm. Nucleus: nucleolus. Note= Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 ma
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDM2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
This MDM2 antibody is useful for Western blot, where a band is seen at ~56 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
MDM2/HDM2 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-02158 in the following application:
Publications
Read Publication using NBP1-02158.

Reactivity Notes

Predicted to react with monkey based on 100% sequence identity.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDM2/HDM2 Antibody

  • ACTFS
  • HDM2
  • HDMX
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • MDM2

Background

MDM2 is an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 transcription factor. MDM2 is a p53 responsive gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDM2/HDM2 Antibody (NBP1-02158)(1)


Reviews for MDM2/HDM2 Antibody (NBP1-02158) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-02158:
Filter by Applications
WB
(2)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot MDM2/HDM2 NBP1-02158
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample TestedSJSA-1 Whole Cell Lysate
SpeciesHuman
LotA
Western Blot MDM2/HDM2 NBP1-02158
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 10/15/2014
View

Summary

ApplicationWestern Blot
Sample TestedSJSA-1 Whole Cell Lysate
SpeciesHuman
LotA

Product General Protocols

View specific protocols for MDM2/HDM2 Antibody (NBP1-02158): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MDM2/HDM2 Antibody (NBP1-02158) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Human Liver Whole Tissue Lysate (Adult Whole Normal)
MDM2/HDM2 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for MDM2/HDM2 Antibody (NBP1-02158)

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Blogs on MDM2/HDM2

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Contact Information

Product PDFs

  NBP1-02158 Specific Protocol
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  PDF Datasheet
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Calculators

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Recent Reviews

5
5
2
4
0
3
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2
0
1
0
Western Blot MDM2/HDM2 NBP1-02158
Enlarge
 Verified Customer
10/16/2014
Application: WB
Species: Human
read this review
Western Blot MDM2/HDM2 NBP1-02158
Enlarge
 Verified Customer
10/15/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol MDM2
Entrez
Uniprot