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Neurogenin-3 Antibody [Biotin]

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Product Details

Summary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF3444
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3444
Carrier Free
AF3444
Carrier Free
AF3444-SP
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Neurogenin-3 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human Neurogenin-3
Met1-Leu214
Accession # Q9Y4Z2
Specificity
Detects human Neurogenin‑3 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NEUROG3
Purity Statement
Antigen Affinity-purified
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Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta  cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NEUROG3