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HNF-6/ONECUT1 Antibody [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HNF-6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by ...read more
HNF-6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 10 µg/mL for 3 hours at room ...read more
HNF‑6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Sheep Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF6277-SP
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HNF-6/ONECUT1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HNF-6/ONECUT1
Gln139-Gly283
Accession # Q9UBC0
Specificity
Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ONECUT1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF6277 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-6/ONECUT1 Antibody [Unconjugated]

  • HNF6
  • HNF-6
  • HNF-6A
  • HNF6Ahepatocyte nuclear factor 6, alpha
  • HNF6hepatocyte nuclear factor 6
  • HNF-6One cut domain family member 1
  • OC-1
  • one cut homeobox 1one cut domain, family member 1
  • ONECUT1

Background

HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha 1AT, alpha -fetoprotein and HNF-3 beta . Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-6/ONECUT1 Antibody (AF6277)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, IHC.


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Flow Cytometry
(1)
IHC
(1)
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(2)
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Showing Publications 1 - 5 of 5.
Publications using AF6277 Applications Species
M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits Sci Rep, 2020-01-22;10(1):996. 2020-01-22 [PMID: 31969659] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Gauhar Masgutova, Audrey Harris, Benvenuto Jacob, Lynn M. Corcoran, Frédéric Clotman Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord Frontiers in Molecular Neuroscience 11/7/2019 [PMID: 31787878]
Audrey Harris, Gauhar Masgutova, Amandine Collin, Mathilde Toch, Maria Hidalgo-Figueroa, Benvenuto Jacob et al. Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord Frontiers in Cellular Neuroscience 6/5/2019 [PMID: 31231191]
KU Kabayiza, G Masgutova, A Harris, V Rucchin, B Jacob, F Clotman The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development Front Mol Neurosci, 2017-05-26;10(0):157. 2017-05-26 [PMID: 28603487] (IHC, Mouse) IHC Mouse
Cédric Francius, María Hidalgo-Figueroa, Stéphanie Debrulle, Barbara Pelosi, Vincent Rucchin, Kara Ronellenfitch et al. Vsx1 Transiently Defines an Early Intermediate V2 Interneuron Precursor Compartment in the Mouse Developing Spinal Cord Frontiers in Molecular Neuroscience 12/26/2016 [PMID: 28082864]

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Bioinformatics

Gene Symbol ONECUT1
Uniprot