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Nestin Antibody (196908) [Unconjugated]

Images

 
Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and ...read more
A172 cells were stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated ...read more
Cardiolipin is externalized to the mitochondrial surface in alpha -syn mutant hNs and transgenic mice in response to alpha -syn accumulation. a Western analysis of lysates from corrected and A53T cells at DIV 14 and ...read more
MET and TMZ effects in in vivo glioma models(A) Variation of U251 tumor volume measured at contrast-enhanced T1 MRI after treatment expressed as percentage of volume change. Error bars indicate SEM. (B) U251 derived ...read more
MET and TMZ effects in in vivo glioma models(A) Variation of U251 tumor volume measured at contrast-enhanced T1 MRI after treatment expressed as percentage of volume change. Error bars indicate SEM. (B) U251 derived ...read more
Neural stem cells could proliferate and differentiate into neurons and astrocytes.(A) Representative photomicrograph of neurospheres in culture. (B) Immunocytochemical staining of purified NSCs with Nestin. (C) ...read more
miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was ...read more
Dissimilar tumorigenesis in the in vivo animal model.(a) Kaplan-Meier survival plots; 3 mice were used for cells of each clone. (b) Representative brain tumors of NOD-SCID mice harboring xenografts of the clones #2 ...read more
Cardiolipin is externalized to the mitochondrial surface in alpha -syn mutant hNs and transgenic mice in response to alpha -syn accumulation. a Western analysis of lysates from corrected and A53T cells at DIV 14 and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
196908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Nestin Antibody (196908) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Specificity
Detects human Nestin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Reviewed Applications
Read 3 Reviews rated 4.3
using
MAB1259 in the following applications:

Publications
Read Publications using
MAB1259 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908) [Unconjugated]

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (MAB1259)(158)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenograft.

We have publications tested in 10 applications: Bioassay, Cell Culture, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Western Blot.


Filter By Application
Bioassay
(1)
Cell Culture
(1)
Flow Cytometry
(9)
ICC
(68)
IF
(2)
IHC
(17)
IHC-Fr
(4)
IHC-P
(8)
Immunocytochemistry
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(97)
Mouse
(8)
Rat
(1)
Xenograft
(2)
All Species
Showing Publications 1 - 10 of 158. Show All 158 Publications.
Publications using MAB1259 Applications Species
Jahn, C;Juchem, M;Sonnenschein, K;Gietz, A;Buchegger, T;Lachmann, N;Göhring, G;Behrens, YL;Bär, C;Thum, T;Hoepfner, J; Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A > T mutation Stem cell research 2024-03-24 [PMID: 38552356] (Flow Cytometry, Immunocytochemistry, Human) Flow Cytometry, Immunocytochemistry Human
Frenz-Wiessner, S;Fairley, SD;Buser, M;Goek, I;Salewskij, K;Jonsson, G;Illig, D;Zu Putlitz, B;Petersheim, D;Li, Y;Chen, PH;Kalauz, M;Conca, R;Sterr, M;Geuder, J;Mizoguchi, Y;Megens, RTA;Linder, MI;Kotlarz, D;Rudelius, M;Penninger, JM;Marr, C;Klein, C; Generation of complex bone marrow organoids from human induced pluripotent stem cells Nature methods 2024-02-19 [PMID: 38374263] (IHC, Human) IHC Human
Gao, L;Behrens, A;Rodschinka, G;Forcelloni, S;Wani, S;Strasser, K;Nedialkova, DD; Selective gene expression maintains human tRNA anticodon pools during differentiation Nature cell biology 2024-01-08 [PMID: 38191669] (ICC, Human) ICC Human
Zimmer, N;Trzeciak, ER;Müller, A;Licht, P;Sprang, B;Leukel, P;Mailänder, V;Sommer, C;Ringel, F;Tuettenberg, J;Kim, E;Tuettenberg, A; Nuclear Glycoprotein A Repetitions Predominant (GARP) Is a Common Trait of Glioblastoma Stem-like Cells and Correlates with Poor Survival in Glioblastoma Patients Cancers 2023-12-05 [PMID: 38136258] (IHC, Xenograft) IHC Xenograft
Patrycja Mulica, Carmen Venegas, Zied Landoulsi, Katja Badanjak, Sylvie Delcambre, Maria Tziortziou et al. Comparison of two protocols for the generation of iPSC-derived human astrocytes Biol Proced Online 2023-09-20 [PMID: 37730545]
Garrudo, FFF;Filippone, G;Resina, L;Silva, JC;Barbosa, F;Ferreira, LFV;Esteves, T;Marques, AC;Morgado, J;Ferreira, FC; Production of Blended Poly(acrylonitrile): Poly(ethylenedioxythiophene):Poly(styrene sulfonate) Electrospun Fibers for Neural Applications Polymers 2023-06-21 [PMID: 37447406] (Human) Human
Jia-jun Qiu, Yan-na Liu, Hao Wei, Fanyi Zeng, Jing-bin Yan Single-cell RNA sequencing of neural stem cells derived from human trisomic iPSCs reveals the abnormalities during neural differentiation of Down syndrome Frontiers in Molecular Neuroscience 6/15/2023 [PMID: 37396785]
Tam, KW;Wong, CY;Wu, KL;Lam, G;Liang, X;Wong, WT;Li, MT;Liu, WY;Cai, S;Shea, GK;Shum, DK;Chan, YS; IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries Cells 2023-05-25 [PMID: 37296600] (ICC, Human) ICC Human
Dell'Amico, C;Angulo Salavarria, MM;Takeo, Y;Saotome, I;Dell'Anno, MT;Galimberti, M;Pellegrino, E;Cattaneo, E;Louvi, A;Onorati, M; Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors eLife 2023-06-05 [PMID: 37272619] (ICC, Human) ICC Human
Karapurkar JK, Kim MS, Colaco JC et al. CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis Journal of experimental & clinical cancer research : CR 2023-05-12 [PMID: 37170124]

Details:
IHC-P 1:100
Show All 158 Publications.

Reviews for Nestin Antibody (MAB1259) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using MAB1259:
Filter by Applications
ICC
(1)
Immunofluorescence - paraffin
(1)
IF
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ICC Human 02/10/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedNeural stem cells
SpeciesHuman
Lot196908
Other Nestin MAB1259
Enlarge
4
reviewed by:
Verified Customer
Immunofluorescence - paraffin Human 03/09/2019
View

Summary

ApplicationOther
Sample Testedhuman glioblastoma
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 heat induced antigen retrieval
  4
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24213561
SpeciesHuman

FAQs for Nestin Antibody (MAB1259) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Nestin Products

Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
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2
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1
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Verified Customer
02/10/2022
Application: ICC
Species: Human

Verified Customer
03/09/2019
Application: Immunofluorescence - paraffin
Species: Human

 
Verified Customer
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NES