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NeuroD1 Antibody [Unconjugated]

 (18 Publications)
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Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2746
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2746-SP
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NeuroD1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562
Specificity
Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NEUROD1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2746 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NeuroD1 Antibody [Unconjugated]

  • basic helix-loop-helix transcription factor
  • BETA2
  • BHF-1
  • BHLHA3
  • bHLHa3beta-cell E-box transactivator 2
  • Class A basic helix-loop-helix protein 3
  • MODY6
  • NeuroD
  • NeuroD1
  • neurogenic differentiation 1
  • neurogenic differentiation factor 1
  • neurogenic helix-loop-helix protein NEUROD
  • NIDDM

Background

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NeuroD1 Antibody (AF2746)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunocytochemistry.


Filter By Application
Flow Cytometry
(1)
ICC
(4)
IHC
(3)
IHC-P
(1)
Immunocytochemistry
(1)
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Human
(6)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF2746 Applications Species
Salas-Lucia, F;Escamilla, S;Bianco, AC;Dumitrescu, A;Refetoff, S; Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie the Allan-Herndon-Dudley syndrome JCI insight 2024-02-20 [PMID: 38376950] (Immunocytochemistry, Human) Immunocytochemistry Human
Jiang, HC;Park, SJ;Wang, IH;Bear, DM;Nowlan, A;Greer, PL; CD20 is a mammalian odorant receptor expressed in a subset of olfactory sensory neurons that mediates innate avoidance of predators bioRxiv : the preprint server for biology 2023-08-10 [PMID: 37609248] (IHC, Mouse) IHC Mouse
Shenghui Liang, Jia Zhao, Robert K. Baker, Elisa Tran, Lisa Zhan, Timothy J. Kieffer Differentiation of stem cell-derived pancreatic progenitors into insulin-secreting islet clusters in a multiwell-based static 3D culture system Cell Reports Methods 2023-05-22 [PMID: 37323565]
Raghu Ram Katreddi, Ed Zandro M. Taroc, Sawyer M. Hicks, Jennifer M. Lin, Shuting Liu, Mengqing Xiang et al. Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents Development 2022-07-01 [PMID: 35781337]
M Jumabay, L Zhang, J Yao, KI Boström Progenitor cells from brown adipose tissue undergo neurogenic differentiation Scientific Reports, 2022-04-04;12(1):5614. 2022-04-04 [PMID: 35379860] (ICC, Mouse) ICC Mouse
NN Nawarawong, KR Thompson, SP Guerin, C Anasooya S, H Peng, K Nixon Reactive, Adult Neurogenesis From Increased Neural Progenitor Cell Proliferation Following Alcohol Dependence in Female Rats Frontiers in Neuroscience, 2021-09-14;15(0):689601. 2021-09-14 [PMID: 34594180] (IHC, Rat) IHC Rat
H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells Nature Communications, 2021-06-07;12(1):3330. 2021-06-07 [PMID: 34099664] (IHC, Human) IHC Human
Manuela Ceccarelli, Giorgio D'Andrea, Laura Micheli, Felice Tirone Deletion of Btg1 Induces Prmt1-Dependent Apoptosis and Increased Stemness in Shh-Type Medulloblastoma Cells Without Affecting Tumor Frequency Frontiers in Oncology 2020-03-13 [PMID: 32231994]
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094658] (ICC, Human) ICC Human
Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020-02-17;21(3):274-286. 2020-02-17 [PMID: 32066947] (ICC, Human) ICC Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol NEUROD1
Uniprot