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Pax6 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and rat cortical stem cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal ...read more
Immersion fixed SA01 human embryonic stem cells were differentiated for 6 days with Recombinant Human Noggin (Catalog # 6057‑NG) and SB431542 (upper panel) or differentiated for 6 days with Recombinant Human BMP-4 ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Pax6 at approximately 59 kDa (as indicated) using 5 µg/mL of Sheep ...read more
Jurkat cells were stained with Sheep Anti-Human Pax6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8150, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Pax6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pax6
Met1-Arg272
Accession # P26367
Specificity
Detects human Pax6 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PAX6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF8150 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax6 Antibody [Unconjugated]

  • keratitis)
  • MGC17209
  • Oculorhombin
  • paired box 6
  • paired box protein Pax-6
  • Pax6

Background

Pax6 (paired box 6; also Oculorhombin) is a 48‑50 kDa member of the paired homeobox family of transcription factors.  It is expressed in developing optic vesicle, olfactory dopaminergic neurons, and pancreatic endocrine cells.  Pax6 is a transactivating protein that interacts with MAF, CDX2 and SOX2.  Human Pax6 is 422 amino acids (aa) in length.  It contains an N-terminal paired box DNA-binding domain (aa 4‑130), a Gly-rich central region (aa 131‑209), a homeodomain (aa 213‑269) and a C-terminal Pro/Ser/Thr-rich regulatory domain (aa 279‑422).  Phosphorylation of the C‑terminal domain at Thr281/304/373 promotes Pax6 activity.  Multiple splice forms of Pax6 exist. There are alternative start sites at Met137 and a position 34 aa upstream of the standard site. There is also a deletion of aa 22‑26 and 37‑39, plus a 14 aa insertion after Gln47 that generates a C‑terminal DNA binding site.  Human and mouse Pax6 are absolutely identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax6 Antibody (AF8150)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, Immunocytochemistry, Western Blot.


Filter By Application
ICC
(4)
IHC
(5)
Immunocytochemistry
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(9)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF8150 Applications Species
Gleixner, S;Zahn, I;Dietrich, J;Singh, S;Drobny, A;Schneider, Y;Schwendner, R;Socher, E;Blavet, N;Bräuer, L;Gostian, AO;Balk, M;Schulze-Tanzil, G;Günther, C;Paulsen, F;Arnold, P; A New Immortalized Human Lacrimal Gland Cell Line Cells 2024-04-03 [PMID: 38607061] (Immunocytochemistry, Human) Immunocytochemistry Human
M Shigyo, Y Kobayashi, O Platoshyn, S Marsala, T Kato, N Takamura, K Yoshida, A Kishino, M Bravo-Hern, S Juhas, J Juhasova, H Studenovsk, V Proks, JD Ciacci, M Marsala Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization Cell Transplantation, 2023-01-01;32(0):9636897231163. 1/1/2023 [PMID: 36959733]
Wang L, Park JY, Liu F et al. A kinase-independent function of cyclin-dependent kinase 6 promotes outer radial glia expansion and neocortical folding Proceedings of the National Academy of Sciences 2022-09-20 [PMID: 36095192]
Zhen Gu, Jia Guo, Jinglei Zhai, Guihai Feng, Xianning Wang, Zili Gao et al. A Uterus‐Inspired Niche Drives Blastocyst Development to the Early Organogenesis Advanced Science 2022-10-01 [PMID: 35843885]
X Dong, Q Zhang, X Yu, D Wang, J Ma, J Ma, SH Shi Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex Nature Neuroscience, 2022-06-20;0(0):. 2022-06-20 [PMID: 35726058] (IHC, Mouse) IHC Mouse
Eriksen A, Moller R, Markovoz B et al. Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids STAR Protocols 4/1/2022 [PMID: 35664254]
You Y, Muraoka S, Jedrychowski MP et al. Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain Journal of Extracellular Vesicles 2022-01-13 [PMID: 35029059]
HS Meharena, A Marco, V Dileep, ER Lockshin, GY Akatsu, J Mullahoo, LA Watson, T Ko, LN Guerin, F Abdurrob, S Rengarajan, M Papanastas, JD Jaffe, LH Tsai Down-syndrome-induced senescence disrupts the nuclear architecture of neural progenitors Cell Stem Cell, 2022-01-06;29(1):116-130.e7. 2022-01-06 [PMID: 34995493] (ICC, Human) ICC Human
M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021-10-01;0(0):. 2021-10-01 [PMID: 34599305] (IHC, Human) IHC Human
AZ Eriksen, R Møller, B Makovoz, SA Uhl, BR tenOever, TA Blenkinsop SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium Cell Stem Cell, 2021-05-17;0(0):. 2021-05-17 [PMID: 34022129] (IHC, Human) IHC Human
Show All 16 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Pax6 Antibody (AF8150). (Showing 1 - 1 of 1 FAQs).

  1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
    • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

Secondary Antibodies

 

Isotype Controls

Additional Pax6 Products

Blogs on Pax6.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol PAX6
Uniprot