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![Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP1-87178-img0017.jpg "Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP1-87178] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87178-img0026.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining in human cerebral cortex and pancreas tissues . Corresponding QKI RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0025.jpg)
![Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP1-87178-img0016.jpg)
![Immunohistochemistry: QKI/Quaking Antibody [NBP1-87178] - Staining of mouse brain shows strong positivity in a subset of glial cells in the cerebral cortex.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0021.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebellum shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0022.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0023.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP1-87178-img0024.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0007.jpg "Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QKI protein stained by QKI antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20029-img0004.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0006.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20029-img0003.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029] - Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP2-20029-img0002.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0007.jpg "Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed MCF7 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of K562 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0006.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed HeLa cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0001.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed K562 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0002.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed U87 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0003.jpg)
![Protein Array: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Analysis of Protein Array containing more than 19,000 full-length human proteins using QKI/Quaking Antibody (PCRP-QKI-2F10).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Protein-Array-NBP3-13817-img0009.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunocytochemistry-Immunofluorescence-NBP3-13575-img0004.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI/Quaking protein stained by QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Frozen-NBP3-13575-img0005.jpg)
![Western Blot: QKI/Quaking Antibody (GT1589) [NBP3-13575] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:3000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Western-Blot-NBP3-13575-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT1589) [NBP3-13575] - QKI/Quaking antibody [GT1589] detects QKI/Quaking protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT1589] (NBP3-13575) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT1589-Immunohistochemistry-Paraffin-NBP3-13575-img0003.jpg)
![Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-240-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-NB300-240-img0006.jpg)
![Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunoprecipitation-NB300-240-img0011.jpg)
![Western Blot: QKI/Quaking Antibody (GT2612) [NBP3-13576] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT2612-Western-Blot-NBP3-13576-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT2612) [NBP3-13576] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT2612) [NBP3-13576] - QKI/Quaking antibody [GT2612] detects QKI/Quaking protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT2612-Immunohistochemistry-Paraffin-NBP3-13576-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT2612) [NBP3-13576] - QKI/Quaking antibody [GT2612] detects QKI/Quaking protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT2612-Immunohistochemistry-Paraffin-NBP3-13576-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT2612) [NBP3-13576] - QKI/Quaking antibody [GT2612] detects QKI/Quaking protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.QKI/Quaking stained by QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13576_mouse-qki-quaking-mab-gt2612-91020242063387.jpg)
![Western Blot: QKI/Quaking Antibody (GT2612) [NBP3-13576] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13576_mouse-qki-quaking-mab-gt2612-91020242021990.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT2612) [NBP3-13576] - QKI/Quaking antibody [GT2612] detects QKI/Quaking protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.QKI/Quaking stained by QKI/Quaking antibody [GT2612] (NBP3-13576) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13576_mouse-qki-quaking-mab-gt2612-91020242091486.jpg)
![Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Western blot analysis of extracts of various cell lines, using QKI/Quaking antibody (NBP3-15255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-1D1N6-Western-Blot-NBP3-15255-img0002.jpg "Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Western blot analysis of extracts of various cell lines, using QKI/Quaking antibody (NBP3-15255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunofluorescence analysis of NIH/3T3 cells using QKI/Quaking Rabbit mAb (NBP3-15255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-1D1N6-Immunocytochemistry-Immunofluorescence-NBP3-15255-img0001.jpg)
![Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug QKI/Quaking antibody (NBP3-15255). Western blot was performed from the immunoprecipitate using QKI/Quaking antibody (NBP3-15255) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-1D1N6-Immunoprecipitation-NBP3-15255-img0003.jpg)
![Western Blot: QKI/Quaking Antibody [NB300-239] - Detection of Human QKI by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-QKI antibody NB300-239 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-239-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-239] - Detection of Human QKI by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-QKI antibody NB300-239 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: QKI/Quaking Antibody [NB300-239] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-239-img0002.jpg)
![Western Blot: QKI/Quaking Antibody (GT228) [NBP3-13577] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT228] (NBP3-13577) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT228-Western-Blot-NBP3-13577-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: QKI/Quaking Antibody (GT228) [NBP3-13577] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with QKI/Quaking antibody [GT228] (NBP3-13577) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT228) [NBP3-13577] - QKI/Quaking antibody [GT228] detects QKI/Quaking protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. QKI/Quaking antibody [GT228] (NBP3-13577) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT228-Immunohistochemistry-Paraffin-NBP3-13577-img0002.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT228) [NBP3-13577] - QKI/Quaking antibody [GT228] detects QKI/Quaking protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. QKI/Quaking antibody [GT228] (NBP3-13577) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-GT228-Immunohistochemistry-Paraffin-NBP3-13577-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT228) [NBP3-13577] - QKI/Quaking antibody [GT228] detects QKI/Quaking protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.QKI/Quaking stained by QKI/Quaking antibody [GT228] (NBP3-13577) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13577_mouse-qki-quaking-mab-gt228-910202420131297.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody (GT228) [NBP3-13577] - QKI/Quaking antibody [GT228] detects QKI/Quaking protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.QKI/Quaking stained by QKI/Quaking antibody [GT228] (NBP3-13577) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13577_mouse-qki-quaking-mab-gt228-9102024205659.jpg)
![Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Western-Blot-NBP2-42196-img0003.jpg "Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI/Quaking (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42196-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42196-img0002.jpg)
![Western Blot: QKI/Quaking Antibody [NB100-41401] - (0.3ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB100-41401-img0002.jpg "Western Blot: QKI/Quaking Antibody [NB100-41401] - (0.3ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: QKI/Quaking Antibody (S01-3F7) [NBP3-19753] - Western blot detection of QKI/Quaking in K562 cell lysates using NBP3-19753 (1:1000 diluted). Predicted band size: 38kDa. Observed band size: 38kDa.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S01-3F7-Western-Blot-NBP3-19753-img0001.jpg "Western Blot: QKI/Quaking Antibody (S01-3F7) [NBP3-19753] - Western blot detection of QKI/Quaking in K562 cell lysates using NBP3-19753 (1:1000 diluted). Predicted band size: 38kDa. Observed band size: 38kDa.")