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QKI/Quaking Antibody

Images

 
Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% ...read more
Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI protein stained ...read more
Western Blot: QKI/Quaking Antibody [NBP2-20029] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029] - Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: QKI protein stained by QKI ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, FeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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QKI/Quaking Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human QKI/Quaking. The exact sequence is proprietary.
Localization
Nucleus (By similarity); Cytoplasm
Predicted Species
Porcine (100%), Bovine (100%), Feline (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
QKI
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for QKI/Quaking Antibody

  • DKFZp586I0923
  • HKQ
  • homolog of mouse quaking QKI (KH domain RNA binding protein)
  • Hqk
  • hqkI
  • protein quaking
  • QK
  • QK1
  • QK3
  • quaking homolog, KH domain RNA binding (mouse)
  • RNA binding protein HQK

Background

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998 [PubMed 9671495]). QKI proteins regulate RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability (Lauriat et al., 2008 [PubMed 17918747]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QKI
Uniprot