![Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Western-Blot-NBP2-42196-img0003.jpg "Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S147-6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b. |
| Localization | Cytoplasm , Nucleus |
| Specificity | Greater then 90% identity with QKI-6, QKI-7 and QKI-7b.Detects approx 36-38kDa. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | QKI |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of QKI (pan) Antibody was sufficient for detection of Pan-QKI in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | QKI |
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI/Quaking (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42196-img0004.jpg "Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI/Quaking (pan) Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-S147-6-Pan-Specific-Immunocytochemistry-Immunofluorescence-NBP2-42196-img0002.jpg "Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.")
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