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QKI/Quaking Antibody (PCRP-QKI-2F10)

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Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat ...read more
Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed MCF7 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control ...read more
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI/Quaking Antibody ...read more
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of K562 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse ...read more
Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed HeLa cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control ...read more
Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed K562 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control ...read more
Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed U87 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control ...read more
Protein Array: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Analysis of Protein Array containing more than 19,000 full-length human proteins using QKI/Quaking Antibody (PCRP-QKI-2F10).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, MA
Clone
PCRP-QKI-2F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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QKI/Quaking Antibody (PCRP-QKI-2F10) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14072)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human QKI/Quaking protein (Uniprot: Q96PU8)
Localization
Nucleus, Cytoplasm
Isotype
IgG2c
Clonality
Monoclonal
Host
Mouse
Gene
QKI
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: concentration is assay dependent

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for QKI/Quaking Antibody (PCRP-QKI-2F10)

  • DKFZp586I0923
  • HKQ
  • homolog of mouse quaking QKI (KH domain RNA binding protein)
  • Hqk
  • hqkI
  • protein quaking
  • QK
  • QK1
  • QK3
  • quaking homolog, KH domain RNA binding (mouse)
  • RNA binding protein HQK

Background

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for QKI/Quaking Antibody (NBP3-13817) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QKI
Uniprot