![Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NB300-240-img0008.jpg "Western Blot: QKI/Quaking Antibody [NB300-240] - Detection of Human and Mouse QKI by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody NB300-240 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to the C-terminus of isoform 5 of human quaking homolog according to entry AAH19917.1 (GeneID 9444). The epitope is not found in isoforms 1, 2, 3, 4, 6, 7 or 7B according to TrEMBL entries Q9P0Y1, Q9P0Y0, Q9P0X6, Q9P0X8, Q96PU7, Q969L9 and Q96PU6, respectively. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | QKI |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | By Western Blot, this antibody recognizes a protein ~38 kDa, representing QKI. Immunoprecipitation was reported in scientific literature. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for QKI/Quaking Antibody (NB300-240)Find related products by research area.
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| Gene Symbol | QKI |
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![Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-NB300-240-img0006.jpg "Immunohistochemistry: QKI/Quaking Antibody [NB300-240] - Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- QKI used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunoprecipitation-NB300-240-img0011.jpg "Immunoprecipitation: QKI/Quaking Antibody [NB300-240] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. antibodies: Affinity purified rabbit anti-QKI antibody used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot of this antibody. For blotting immunoprecipitated QKI, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")