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QKI/Quaking Antibody

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Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody [NBP1-87178] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining in human cerebral cortex and pancreas tissues . Corresponding QKI RNA-seq data are presented for the same tissues.
Western Blot: QKI/Quaking Antibody [NBP1-87178] - Analysis in human cerebral cortex tissue.
Immunohistochemistry: QKI/Quaking Antibody [NBP1-87178] - Staining of mouse brain shows strong positivity in a subset of glial cells in the cerebral cortex.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebellum shows moderate to strong nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP1-87178] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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QKI/Quaking Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
QKI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
QKI/Quaking Protein (NBP1-87178PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for QKI/Quaking Antibody

  • DKFZp586I0923
  • HKQ
  • homolog of mouse quaking QKI (KH domain RNA binding protein)
  • Hqk
  • hqkI
  • protein quaking
  • QK
  • QK1
  • QK3
  • quaking homolog, KH domain RNA binding (mouse)
  • RNA binding protein HQK

Background

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol QKI