Immunohistochemistry Resources

Read our immunohistochemistry protocols and troubleshooting guides to understand IHC sample preparation, fixation, blocking, epitope retrieval, staining, and IHC detection. Our comprehensive resources cover both IHC staining of paraffin-embedded and frozen tissue sections.

Protocols

• Dual ISH-IHC Protocol
• Chromogenic Staining of FFPE Tissue Protocol
• Chromogenic Staining of Frozen Tissue Protocol
• Fluorescent Staining of FFPE Tissue Protocol
• Fluorescent Staining of Frozen Tissue Protocol
• IHC Troubleshooting Guide (FFPE)
• IHC Troubleshooting Guide (Frozen)
• Antigen Retrieval (HIER)
• Antigen Retrieval (PIER)
• Antigen Retrieval (Frozen Sections)
• Antigen Retrieval (Chinese)

Other

• Understanding Peptide Blocking
• Peptide Blocking Protocol
• ICC vs IHC vs IF

Understanding IHC

• Sample Preparation (Fr vs. P) for IHC
• Sample Fixation for IHC
• Blocking Non-Specific Binding in IHC
• Selecting a Primary Antibody for IHC
• Epitope Retrieval (HIER vs. PIER)
• Detection Methods for IHC
• Immunohistochemistry (IHC) Handbook

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