Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ce,
Ch,
Dr,
Fi,
Gp,
Ha,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Dr, Fi, Gp, Ha, Pm, Rb, Sh
WB, ELISA, Flow, ICC/IF, IHC, MA, MS, Binding Activity
Applications: WB, ELISA, Flow, ICC/IF, IHC, MA, MS, Binding Activity
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-C92F3A-5-Western-Blot-NB110-61582-img0018.jpg "Western Blot: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (NB110-61582). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-61582) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-C92F3A-5-Immunohistochemistry-NB110-61582-img0020.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (NB110-61582). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (NB110-61582) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-C92F3A-5-Flow-Cytometry-NB110-61582-img0021.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Ceter and Inst. Of Oncology, Poland.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-C92F3A-5-Immunohistochemistry-Paraffin-NB110-61582-img0016.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (C92F3A-5) [NB110-61582] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (NB110-61582). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-61582) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-C92F3A-5-Immunohistochemistry-NB110-61582-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77455-img0009.jpg "Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-NBP1-77455-img0010.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0014.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - Single cell suspension generated from B16-F10 tumor excised from C57BL/6 Mouse stained with Hsp70 & FITC secondary (blue) or unstained (red). [Image provided by a varified research professional; see Product Reviews for details on protocol employed].](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0011.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on A549 cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0013.jpg)
![Simple Western: HSP70/HSPA1A Antibody [NBP1-77455] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Simple-Western-NBP1-77455-img0012.jpg)
![Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-BB70-Western-Blot-NB110-96427-img0016.jpg "Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSPA1A cells stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-BB70-Immunohistochemistry-NB110-96427-img0018.jpg)
![Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-BB70-Western-Blot-NB110-96427-img0013.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Detection in paraffin embedded liver sections and nuclear sections of rat hepatocytes by staining with BB70. First pictures in series show two hours after exposure to stress, the second shows the control.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-BB70-Immunohistochemistry-NB110-96427-img0008.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-BB70-Immunohistochemistry-Paraffin-NB110-96427-img0015.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSPA1A stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-BB70-Immunohistochemistry-NB110-96427-img0017.jpg)
![Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).](http://images.novusbio.com/fullsize/Hsp70---Hsc70-Antibody-Western-Blot-NBP1-97872-img0003.jpg "Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).")
![Immunohistochemistry-Paraffin: HSP70 / HSC70 Antibody [NBP1-97872] - Analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/HSP70---HSC70-Antibody-Immunohistochemistry-Paraffin-NBP1-97872-img0001.jpg)