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6 Results for "novus"

AF562

MuSK Antibody

(16 Publications)

100 μg

AF562

Recombinant Rat Agrin (Catalog # 550-AG) induces MuSK-dependent acetylcholine receptor clustering on myotubes differentiated from the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recombinant Rat Agrin (16 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat MuSK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF562). The ND50 is typically 0.5-2 µg/mL.

MuSK patient IgG4 or IgG1-3 do not induce endocytosis of MuSK.Patient plasma or purified IgG1-3 or IgG4 were applied at 0.17nM final concentration of MuSK antibody to HEK293 cells expressing MuSK at the cell surface. Cells were either incubated at 4°C to prevent, or at 37°C to allow, endocytosis. Patient antibody binding was visualised by addition of a secondary fluorescent anti-human antibody at the end of the experiment. (A) An example of cells that were incubated with IgG1-3 from patient 9. Robust staining was observed after 6 hours incubation at both temperatures. Scale bar=25µm. (B) Staining was scored by two individuals as described in methods, and the scores were normalised to the score at 0 hours for each condition. A slight decrease in staining was observed for cells incubated at 37°C, and pre-fixed cells also showed this reduction. (C) As a positive control for endocytosis, IgG1-3 or IgG4 was cross-linked by addition of Alexa Fluor 568-conjugated anti-human IgG prior to incubation. Example images show cells treated with patient 9 IgG4 and IgG1-3 in the presence and absence of cross-linking anti-human IgG after 6 hours incubation at 37°C. There is a clear difference when the cross-linking secondary antibody is present. Scale bar =50µm (D) Cross-linking by the secondary antibody induced internalisation of human anti-MuSK antibodies as well as MuSK-EGFP as early as 30 minutes, as observed by confocal microscopy. An example image of a cell from a confocal z-stack with orthogonal side-views is shown. The arrow shows internalised secondary Alexa Fluor 568-conjugated anti-human IgG (red) colocalised with EGFP-tagged MuSK (green). Scale bar =5µm. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0080695), licensed under a CC-BY license. Not internally tested by R&D Systems.

MuSK patient IgG4 or IgG1-3 do not induce endocytosis of MuSK.Patient plasma or purified IgG1-3 or IgG4 were applied at 0.17nM final concentration of MuSK antibody to HEK293 cells expressing MuSK at the cell surface. Cells were either incubated at 4°C to prevent, or at 37°C to allow, endocytosis. Patient antibody binding was visualised by addition of a secondary fluorescent anti-human antibody at the end of the experiment. (A) An example of cells that were incubated with IgG1-3 from patient 9. Robust staining was observed after 6 hours incubation at both temperatures. Scale bar=25µm. (B) Staining was scored by two individuals as described in methods, and the scores were normalised to the score at 0 hours for each condition. A slight decrease in staining was observed for cells incubated at 37°C, and pre-fixed cells also showed this reduction. (C) As a positive control for endocytosis, IgG1-3 or IgG4 was cross-linked by addition of Alexa Fluor 568-conjugated anti-human IgG prior to incubation. Example images show cells treated with patient 9 IgG4 and IgG1-3 in the presence and absence of cross-linking anti-human IgG after 6 hours incubation at 37°C. There is a clear difference when the cross-linking secondary antibody is present. Scale bar =50µm (D) Cross-linking by the secondary antibody induced internalisation of human anti-MuSK antibodies as well as MuSK-EGFP as early as 30 minutes, as observed by confocal microscopy. An example image of a cell from a confocal z-stack with orthogonal side-views is shown. The arrow shows internalised secondary Alexa Fluor 568-conjugated anti-human IgG (red) colocalised with EGFP-tagged MuSK (green). Scale bar =5µm. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0080695), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF562

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF562-SP

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NB120-5619

MuSK Antibody

(1 Publication)

200 μg

NB120-5619

Western Blot: MuSK Antibody [NB120-5619] - Analysis of 3ng of muscle-specific kinase antigen and 10ul PageRuler Plus Prestained Protein Ladder per well.

Immunocytochemistry/Immunofluorescence: MuSK Antibody [NB120-5619] - Analysis of muscle-specific kinase (MuSK) (green) in C2C12 mouse myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody, at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary antibody for 45 minutes at room temperature (green). F-Actin (both panels, red) was stained with DyLight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

Immunohistochemistry-Paraffin: MuSK Antibody [NB120-5619] - Staining on mouse skeletal muscle tissue.

Western Blot: MuSK Antibody [NB120-5619] - Analysis of Rat MuSK transfected COS7 cell lysate.

Western Blot: MuSK Antibody [NB120-5619] - Analysis of 30ug of rat skeletal muscle tissue lysate and 10ul PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.

Immunocytochemistry/Immunofluorescence: MuSK Antibody [NB120-5619] - Analysis of muscle-specific kinase (MuSK) (green) in L6 rat myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody, at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary antibody for 45 minutes at room temperature (green). F-Actin (both panels, red) was stained with DyLight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

Immunohistochemistry-Paraffin: MuSK Antibody [NB120-5619] - Staining on rat skeletal muscle tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB562

MuSK Antibody (78020) [Unconjugated]

500 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB562-SP

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9847-MK

Recombinant Rat MuSK Fc Chimera Protein, CF

50 μg

9847-MK

When Recombinant Rat MuSK Fc Chimera (Catalog #9847-MK) is coated at 2 μg/mL, Recombinant Human LRP-4 (Catalog # 5948-LR) binds with an ED50 of 0.06-0.3 μg/mL in the presence of Recombinant Rat Agrin (Catalog # 550-AG).

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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NBP2-15028

MuSK Antibody

0.1 ml

NBP2-15028

Western Blot: MUSK Antibody [NBP2-15028] - Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: MUSK Antibody [NBP2-15028] - Paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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BAF562

MuSK Antibody [Biotin]

50 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF562
Carrier Free	AF562-SP

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