| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80561 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-66486-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-65674B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64526-0.025mg | |||
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0019.jpg "Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 5 ug/mL overnight at 4C and detected with an anti-Mouse IgM DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0018.jpg)
![Simple Western: Actin Antibody (mAbGEa) [NB100-74340] - Image shows a specific band for Actin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Simple-Western-NB100-74340-img0014.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0005.jpg)
![Flow Cytometry: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Flow-Cytometry-NB100-74340-img0015.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Increased relative abundance of Park7 in Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for the Park7 protein. Pooled liver protein extracts (n = 4 livers per pool) were separated by SDS PAGE in multiple replicates (n = 7) and transferred onto nitrocellulose membrane. Membranes were probed with an anti-Park7 antibody using an anti-actin antibody as a loading control. Liver protein extracts were the same as those used for Figure 4(a) as well as fresh liver protein extracts from new mice of the same age, sex, and genotype. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Actin Antibody (mAbGEa) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0021.jpg)
![Flow (Intracellular): Actin Antibody (mAbGEa) [NB100-74340] - An intracellular stain was performed on A549 cells with NB100-74340 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Flow-Intracellular-NB100-74340-img0020.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0008.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - Actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB100-74340) at 1:1800. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 2 ug/mL overnight at 4C and detected with an anti-mouse IgM DyLight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0017.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0010.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0004.jpg)
![Analysis of Ia production by engineered S. cerevisiae. (B) Production of Ia by the wild type S. cerevisiae strain. Wild type yeast strains harboring the Ia-containing plasmid (Ia) or the control vector (vector) were cultivated in glucose-containing liquid medium until OD595 = 0.5. Afterwards, glucose was replaced by galactose and cultivation continued for 9 h at 30C. Cells were lysed and the resulting extract preparations were ADP-ribosylated in the presence of Ia (+ Ia), TccC3 toxin of P. luminescens [42] (+ TccC3), purified muscle actin (+alpha-actin) or tested in Western blotting with the anti-actin serum to show equal actin concentrations in the samples. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145708), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-291220231223247.jpg)
![Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Western-Blot-NB500-574-img0005.jpg "Western Blot: gamma Tubulin Antibody (TU-30) [NB500-574] - Western blotting analysis of differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.")
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of HeLa cells using anti-gamma Tubulin antibody (green). Nuclei were stained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunofluorescence-NB500-574-img0004.jpg)
![Immunohistochemistry-Paraffin: gamma Tubulin Antibody (TU-30) - BSA Free [NB500-574] - FFPE Glioblastoma PDX is stained with mouse-gamma tubulin antibody (NB500-574) (red) for centrosomes and rabbit Arl13B (green) for primary cilia. Gamma Tubulin antibody is diluted 1:25, incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-BSA-Free-Immunohistochemistry-Paraffin-NB500-574-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunocytochemistry-Immunofluorescence-NB500-574-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunocytochemistry-Immunofluorescence-NB500-574-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (TU-30) [NB500-574] - Staining of microtubular networks in 3T3 mouse fibroblasts. 3A - metaphase; 3B - anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-30-Immunocytochemistry-Immunofluorescence-NB500-574-img0003.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody (M02), clone 4H3.Lane 1: RPS5 transfected lysate(22.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0014.jpg "Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody (M02), clone 4H3.Lane 1: RPS5 transfected lysate(22.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of monoclonal antibody to RPS5 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Immunocytochemistry-Immunofluorescence-H00006193-M02-img0008.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3 Analysis of RPS5 expression in HeLa.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0010.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in PC-12.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0011.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in Raw 264.7.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0012.jpg)
![Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in NIH/3T3.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-Western-Blot-H00006193-M02-img0013.jpg)
![ELISA: RPS5 Antibody (4H3) [H00006193-M02] - Detection limit for recombinant GST tagged RPS5 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RPS5-Antibody-4H3-ELISA-H00006193-M02-img0009.jpg)
![Flow Cytometry: Toxoplasma Gondii P30 Antibody (P30/3) [NB100-66486] - analysis of Toxoplasma Gondii P30 in human foreskin fibroblasts using anti-oxoplasma Gondii P30 antibody. Sample was infected with RH Toxoplasma gondii. Image from verified customer review.](http://images.novusbio.com/fullsize/Toxoplasma-Gondii-P30-Antibody-P30-3-Flow-Cytometry-NB100-66486-img0001.jpg "Flow Cytometry: Toxoplasma Gondii P30 Antibody (P30/3) [NB100-66486] - analysis of Toxoplasma Gondii P30 in human foreskin fibroblasts using anti-oxoplasma Gondii P30 antibody. Sample was infected with RH Toxoplasma gondii. Image from verified customer review.")
![Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0004.jpg "Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot analysis. Whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 and P31r cells. Proteins (90 ug) were resolved in 10% SDS-PAGE gels and transferred to a PVDF membrane. Blots were probed for SIRT1, PGC1alpha, TFAM and SIRT3 or the loading control gamma-tubulin. Figure shows representative blots of (A) of SIRT1, (B) PGC1alpha, (C) SIRT3, (D) TFAM protein expression; each from three independent experiments. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885) licensed under a CC-BY license.")
![Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0001.jpg)
![Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.](http://images.novusbio.com/fullsize/gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0003.jpg)
![Western Blot: HSP65 (mycobacterial) Antibody (3F7) [NBP1-97880] - Analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E. coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.](http://images.novusbio.com/fullsize/HSP65-mycobacterial-Antibody-3F7-Western-Blot-NBP1-97880-img0001.jpg "Western Blot: HSP65 (mycobacterial) Antibody (3F7) [NBP1-97880] - Analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E. coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.")
![Flow Cytometry: Cryptosporidium Antibody (BEL 0126) [NB100-64526] - Analysis using the FITC conjugate of NB100-64526. Staining of Cryptosporidium parvum oocytes.](http://images.novusbio.com/fullsize/Cryptosporidium-Antibody-BEL-0126-Flow-Cytometry-NB100-64526-img0003.jpg "Flow Cytometry: Cryptosporidium Antibody (BEL 0126) [NB100-64526] - Analysis using the FITC conjugate of NB100-64526. Staining of Cryptosporidium parvum oocytes.")
![Western Blot: Tbj51 Antibody [NBP3-18235] - Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~80 kDa Tbj51 protein using Rabbit Anti-Tbj51 Polyclonal Antibody (NBP3-18235). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37C. Lane 3: Cells incubated for 1 hour at 42C. Lane 4: Purified recombinant Tbj51 in E. coli. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Tbj51 Polyclonal Antibody (NBP3-18235) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~80 kDa.](http://images.novusbio.com/fullsize/Tbj51 Antibody-Western Blot-NBP3-18235-img0001.jpg "Western Blot: Tbj51 Antibody [NBP3-18235] - Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~80 kDa Tbj51 protein using Rabbit Anti-Tbj51 Polyclonal Antibody (NBP3-18235). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37C. Lane 3: Cells incubated for 1 hour at 42C. Lane 4: Purified recombinant Tbj51 in E. coli. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Tbj51 Polyclonal Antibody (NBP3-18235) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~80 kDa.")
![Western Blot: TbHsp70 Antibody [NBP3-18236] - Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (NBP3-18236). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37C. Lane 3: Cells incubated for 1 hour at 42C. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (NBP3-18236) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.](http://images.novusbio.com/fullsize/TbHsp70 Antibody-Western Blot-NBP3-18236-img0001.jpg "Western Blot: TbHsp70 Antibody [NBP3-18236] - Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody (NBP3-18236). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37C. Lane 3: Cells incubated for 1 hour at 42C. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody (NBP3-18236) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.")