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![Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391] - Rat heart. Cardiac Troponin T stained by Cardiac Troponin T antibody (NBP3-13391) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Immunohistochemistry-Paraffin-NBP3-13391-img0003.jpg)
![Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0001.jpg)
![Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0004.jpg)
![Western Blot: Cardiac Troponin T Antibody [NBP3-13391] - Human heart (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13391-img0005.jpg)
![Immunohistochemistry-Paraffin: Cardiac Troponin T Antibody [NBP3-13391] - Mouse heart. Cardiac Troponin T stained by Cardiac Troponin T antibody (NBP3-13391) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Immunohistochemistry-Paraffin-NBP3-13391-img0002.jpg)
![Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP1-88070-img0015.jpg "Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Staining of human skeletal muscle shows very weak positivity in myocytes.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Analysis in human heart muscle and liver tissues. Corresponding TNNT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP1-88070-img0011.jpg)
![Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP1-88070-img0012.jpg)
![Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP1-88070-img0013.jpg)
![Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP1-88070-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody [NBP1-88070] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody (A) NBP1-88070 shows similar protein distribution across tissues to independent antibody NBP1-88069 (B).](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-Immunohistochemistry-Paraffin-NBP1-88070-img0016.jpg)
![Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-1C11-Sandwich-ELISA-NB200-412-img0002.jpg "Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).")
![Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13030-img0003.jpg "Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13030-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Cardiac Troponin T Antibody [NBP3-13030] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membranes were blotted with Cardiac Troponin T antibody (NBP3-13030) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Cardiac-Troponin-T-Antibody-Western-Blot-NBP3-13030-img0002.jpg)