Hu,
Mu,
Rt,
Am,
Av,
Ma,
Sh,
Bv,
Ca,
Ch,
Eq,
Fe,
Av-Bi,
Gt,
Gp,
Ha,
Pm,
Rb,
XpSpecies Glossary
Reactivity: Hu, Mu, Rt, Am, Av, Ma, Sh, Bv, Ca, Ch, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Xp
WB, Simple Western, ICC/IF, IHC
Applications: WB, Simple Western, ICC/IF, IHC
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Western-Blot-NBP1-30054-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.")
![Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Immunohistochemical staining targeting Collagen I alpha 1 revealed severe disorganization of the annulus fibrosus (AF), and a decrease in staining intensity for group D. On the contrary, the arcshaped and layered AF structure with immunoreactivity for type I collagen staining were mostly maintained in group S when compared to group C. Presented images show the AF at site of surgery, defect, and transplant. Abbreviations: D: AF defect only group, S: AF defect treated with sheet transplantation group, C: sham group, NC: negative control of sample stained without primary antibody (scale bar=300um). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31463464/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30054-img0006.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30054-img0004.jpg)
![Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Simple-Western-NBP1-30054-img0003.jpg)
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0008.jpg "Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from soleus muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0009.jpg)
![Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30042-img0003.jpg)
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0004.jpg)
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from cardiac muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression within the infarct region of hearts from Postn^MCM/+; R26^EGFP mice versus Postn^MCM/MCM; R26^EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2 Antibody - C-terminus - Azide and BSA Free-Immunohistochemistry-NBP1-30042-img0010.jpg)
![Knockout Validated: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and periostin from isolated infarct areas of the hearts of the indicated groups of mice 7 days after MI. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0181945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Knockout-Validated-NBP1-30042-img0005.jpg)
![Western Blot: P4HA1 Antibody [H00005033-B01P] - Analysis of P4HA1 expression in transfected 293T cell line by P4HA1 polyclonal antibody. Lane 1: P4HA1 transfected lysate(58.74 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/P4HA1-Antibody-Western-Blot-H00005033-B01P-img0003.jpg "Western Blot: P4HA1 Antibody [H00005033-B01P] - Analysis of P4HA1 expression in transfected 293T cell line by P4HA1 polyclonal antibody. Lane 1: P4HA1 transfected lysate(58.74 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: P4HA1 Antibody [H00005033-B01P] - P4HA1 MaxPab polyclonal antibody. Western Blot analysis of P4HA1 expression in A-431.](http://images.novusbio.com/fullsize/P4HA1-Antibody-Western-Blot-H00005033-B01P-img0002.jpg)
![Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.](http://images.novusbio.com/fullsize/TAO2-[p-Ser181]-Antibody-Western-Blot-NB300-233-img0005.jpg "Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.")
![Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat cortex lysate showing specific immunolabeling of the ~120k TAO2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubatede (1200 units for 30 min) before being exposed to the Ser181 TAO2 antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/TAO2-[p-Ser181]-Antibody-Western-Blot-NB300-233-img0004.jpg)
![Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP2-29651-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.")
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP2-29651] - Collagen I Antibody [NBP2-29651] - fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-NBP2-29651-img0004.jpg)
![Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PTPN3-[p-Ser459]-Antibody-Western-Blot-NBP2-29518-img0002.jpg "Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/GAP43-[p-Ser41]-Antibody-Western-Blot-NB300-179-img0002.jpg "Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.")
![Western Blot: CtIP [p Thr847] Antibody [NBP2-60770] - Western blot of human T47D cell lysate showing specificimmunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+)where immunolabeling is completely eliminated by blot treatmentwith lambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/CtIP-[p-Thr847]-Antibody-Western-Blot-NBP2-60770-img0001.jpg "Western Blot: CtIP [p Thr847] Antibody [NBP2-60770] - Western blot of human T47D cell lysate showing specificimmunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+)where immunolabeling is completely eliminated by blot treatmentwith lambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")