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![Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/NEK2-Antibody-3B7-Western-Blot-H00004751-M11-img0011.jpg "Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of monoclonal antibody to NEK2 on HeLa cell. Antibody concentration 25 ug/ml](http://images.novusbio.com/fullsize/NEK2-Antibody-3B7-Immunocytochemistry-Immunofluorescence-H00004751-M11-img0007.jpg)
![Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/NEK2-Antibody-3B7-Western-Blot-H00004751-M11-img0008.jpg)
![Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/NEK2-Antibody-3B7-Western-Blot-H00004751-M11-img0009.jpg)
![Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/fullsize/NEK2-Antibody-3B7-Western-Blot-H00004751-M11-img0010.jpg)
![ELISA: NEK2 Antibody (3B7) [H00004751-M11] - Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK2-Antibody-3B7-ELISA-H00004751-M11-img0006.jpg)
![Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Western-Blot-H00004751-M01-img0009.jpg "Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Immunohistochemistry-Paraffin-H00004751-M01-img0006.jpg)
![Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 transfected lysate using anti-NEK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEK2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Immunoprecipitation-H00004751-M01-img0007.jpg)
![Sandwich ELISA: NEK2 Antibody (2F6) [H00004751-M01] - Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F6-Sandwich-ELISA-H00004751-M01-img0008.jpg)
![Western Blot: NEK2 Antibody (2F9) [H00004751-M02] - NEK2 monoclonal antibody (M02), clone 2F9 Analysis of NEK2 expression in PC-12.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F9-Western-Blot-H00004751-M02-img0009.jpg "Western Blot: NEK2 Antibody (2F9) [H00004751-M02] - NEK2 monoclonal antibody (M02), clone 2F9 Analysis of NEK2 expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: NEK2 Antibody (2F9) [H00004751-M02] - Analysis of monoclonal antibody to NEK2 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F9-Immunocytochemistry-Immunofluorescence-H00004751-M02-img0007.jpg)
![Western Blot: NEK2 Antibody (2F9) [H00004751-M02] - WB analysis of NEK2 in human whole cell lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F9-Western-Blot-H00004751-M02-img0005.jpg)
![Sandwich ELISA: NEK2 Antibody (2F9) [H00004751-M02] - Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK2-Antibody-2F9-Sandwich-ELISA-H00004751-M02-img0008.jpg)
![Western Blot: NEK2 Antibody [NBP2-19510] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NEK2-Antibody-Western-Blot-NBP2-19510-img0001.jpg "Western Blot: NEK2 Antibody [NBP2-19510] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: NEK2 Antibody [NBP2-19510] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NEK2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/NEK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19510-img0002.jpg)
![Immunohistochemistry-Paraffin: NEK2 Antibody [NBP2-19510] - Paraffin-embedded human ovarian carcinoma. NEK2 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/NEK2-Antibody-Immunohistochemistry-Paraffin-NBP2-19510-img0003.jpg)
![Western Blot: NEK2 Antibody [NBP1-78022] - Western blot analysis is shown using Affinity Purified NEK2 antibody to detect enCanineenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/NEK2-Antibody-Western-Blot-NBP1-78022-img0005.jpg "Western Blot: NEK2 Antibody [NBP1-78022] - Western blot analysis is shown using Affinity Purified NEK2 antibody to detect enCanineenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.")
![Western Blot: NEK2 Antibody [NBP1-78022] - Western Blot of NEK2 antibody. Lane 1: Opal Pre-stained Molecular Weight Marker. Lane 2: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10ug). Primary Antibody: Anti-NEK2 at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30mins at RT. Block: BlockOut Buffer. Expect/Observed MW: ~76kDa rec NEK2.](http://images.novusbio.com/fullsize/NEK2-Antibody-Western-Blot-NBP1-78022-img0004.jpg)
![N/A: NEK2 Antibody [NBP1-78022]](http://images.novusbio.com/fullsize/NEK2-Antibody-N-A-NBP1-78022-img0003.jpg)
![Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NEK2-Antibody-5O0A1-Western-Blot-NBP3-16641-img0001.jpg "Western Blot: NEK2 Antibody (5O0A1) [NBP3-16641] - Western blot analysis of extracts of various cell lines, using NEK2 Rabbit mAb (NBP3-16641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: NEK2 Antibody (1B6) [NBP1-41207] - Detection of human NEK2 protein in Jurkat whole cell extract.](http://images.novusbio.com/fullsize/NEK2-Antibody-1B6-Western-Blot-NBP1-41207-img0001.jpg "Western Blot: NEK2 Antibody (1B6) [NBP1-41207] - Detection of human NEK2 protein in Jurkat whole cell extract.")
![Immunocytochemistry/Immunofluorescence: NEK2 Antibody [NBP2-57424] - Staining of human cell line RH-30 shows localization to nucleoplasm & centrosome.](http://images.novusbio.com/fullsize/NEK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57424-img0001.jpg "Immunocytochemistry/Immunofluorescence: NEK2 Antibody [NBP2-57424] - Staining of human cell line RH-30 shows localization to nucleoplasm & centrosome.")
![Western Blot: NEK2 Overexpression Lysate (Adult Normal) [NBP2-08109] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEK2.](http://images.novusbio.com/fullsize/NEK2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08109-img0001.jpg "Western Blot: NEK2 Overexpression Lysate (Adult Normal) [NBP2-08109] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEK2.")
![Western Blot: NEK2 Antibody (S03-3D8) [NBP3-15065] - Western blot detection of NEK2 in Hela, A549, HL-60 cell lysates using NEK2 Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/NEK2-Antibody-S03-3D8-Western-Blot-NBP3-15065-img0001.jpg "Western Blot: NEK2 Antibody (S03-3D8) [NBP3-15065] - Western blot detection of NEK2 in Hela, A549, HL-60 cell lysates using NEK2 Rabbit mAb (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")