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![Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Western-Blot-NB120-19294-img0005.jpg "Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) expression and localization in primary neurons. Colocalization of transfected CathD with LAMP-1/CD107a. CathD transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathD. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Lamp1 (red) in HeLa cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin B (CathB) expression and localization in primary neurons. Colocalization of transfected CathB with LAMP-1/CD107a. CathB transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathB. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-52721-img0005.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all the ductal and tubular epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-52721-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-52721-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-52721-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all cells, especially the ductal and tubular epithelial cells. Few cells from the glomeruli showed nuclear signal also.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5E7-Immunohistochemistry-Paraffin-NBP2-52721-img0002.jpg)
![Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Western-Blot-NBP2-25183-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - HeLa cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-25183-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - IHC staining of LAMP-1 [H4A3] catalog number NBP2-25183 in mouse liver. Cytoplasmic and membrane staining was observed in tubule cells and glomeruli.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunohistochemistry-Paraffin-NBP2-25183-img0014.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0017.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human kidney.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Immunohistochemistry-Paraffin-NBP2-25183-img0006.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0009.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0004.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0008.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0010.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) APC.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0011.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor (R) 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-NBP2-25183-img0012.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0013.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A431 cells with LAMP-1/CD107a Antibody [H4A3] NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0015.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-H4A3-Azide-Free-Flow-Cytometry-NBP2-25183-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0007.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of HeLa cells stained with mouse LAMP-1/CD107a mAb, dilution 1:500 (Red), and costained with chicken Vimentin pAb, dilution 1:10000, (Green). DAPI staining of nuclear DNA (Blue). The cells were treated with 50uM of chloroquine, an inhibitor of autophagy, for 16hrs prior to staining. The LAMP-1/CD107a antibody reveals vesicular staining of LAMP1 protein accumulated in swollen lysosomes, while the Vimentin antibody specifically labels the intermediate filament network in these cells.")
![Western Blot: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - Analysis of different cell lysates using mouse mAb to LAMP1, NBP2-25154 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50uM of chloroquine (CQ), an inhibitor of autophagy, for 24 hours: [1] protein standard (red), [2] HeLa Ct, [3] HeLa+CQ, [4] NIH-3T3 Ct, and [5] NIH-3T3+CQ. The smeared band between 75-120kDa corresponds to variably glycosylated forms of the LAMP1 protein detected only in the human cells, this antibody fails to recognize the rodent homologue. The same blot was probed with chicken pAB to HSP60, dilution 1:20,000. The HSP60 antibody reveals heat shock 60 protein with apparent molecular weight of 60kDa in all preparations.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Western-Blot-NBP2-25154-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5H6) [NBP2-25154] - D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for immunofluorescent staining of lysosomal (LAMP-1/CD107a, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04070-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0008.jpg)
![Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Western-Blot-NBP2-59780-img0009.jpg "Western Blot: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human fallopian tube shows granular cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human endometrium shows granular cytoplasmic immunoreactivity in epithelial and stromal cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human prostate shows strong granular cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-3916-Immunohistochemistry-Paraffin-NBP2-59780-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0006.jpg "Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, FITC conjugate.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-956-img0005.jpg)
![Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Western-Blot-NBP3-13609-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - HeLa whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - LAMP-1/CD107a antibody [GT25212] detects LAMP-1/CD107a protein at lysosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: LAMP-1/CD107a stained by LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:2000. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Immunocytochemistry-Immunofluorescence-NBP3-13609-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - Rat liver. LAMP-1/CD107a stained by LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Immunohistochemistry-Paraffin-NBP3-13609-img0004.jpg)
![Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Western-Blot-NBP3-13609-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - Wild-type (WT) and LAMP-1/CD107a knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Western-Blot-NBP3-13609-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (GT25212) [NBP3-13609] - Mouse liver. LAMP-1/CD107a stained by LAMP-1/CD107a antibody [GT25212] (NBP3-13609) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-GT25212-Immunohistochemistry-Paraffin-NBP3-13609-img0003.jpg)
![Western Blot: LAMP-1/CD107a Antibody [NBP1-77241] - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Western-Blot-NBP1-77241-img0011.jpg "Western Blot: LAMP-1/CD107a Antibody [NBP1-77241] - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NBP1-77241] - Staining of rat colon tissue with antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77241-img0009.jpg)
![Immunohistochemistry: LAMP-1/CD107a Antibody [NBP1-77241] - Staining of rat colon tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunohistochemistry-NBP1-77241-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NBP1-77241] - LAMP-1 in Human Colon cells with LAMP-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77241-img0008.jpg)
![Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0006.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human kidney shows strong granular cytoplasmic immunoreactivity in renal glomerulus and tubules cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human fallopian tube shows granular cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0005.jpg)
![Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDa Observed band size:120 kDa](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Western-Blot-NBP2-89844-img0005.jpg "Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDa Observed band size:120 kDa")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LAMP1 monoclonal antibody (1:300). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunocytochemistry-Immunofluorescence-NBP2-89844-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0002.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated, stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Flow-Cytometry-NBP2-89844-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0001.jpg)
![Immunoprecipitation: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Lane A:0.5 mg Jurkat Whole Cell Lysate 1 uL anti-LAMP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LAMP1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 113 kDa](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunoprecipitation-NBP2-89844-img0006.jpg)
![LAMP-1/CD107a was immunoprecipitated using NBP2-89844 at a 1:100 dilution and 60ug Immunomagnetic beads Protein A/G and Goat Anti-Rabbit IgG (H+L)[HRP] as a secondary antibody at 1/10000 dilution: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg Daudi Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-4520231646141.png)
![Western Blot: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Western-Blot-NBP2-59053-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Analysis in human cell line RT-4.")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human skeletal muscle shows only weak cytoplasmic positivity in muscle fibers.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human cervix shows granular cytoplasmic immunoreactivity in squamous epithelium cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human kidney shows strong granular cytoplasmic positivity in renal tubules and glomeruli.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human rectum shows granular cytoplasmic immunoreactivity in glandular cells and in lymphoid cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human endometrium shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous tubules.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0005.jpg)
![Western Blot: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4463-Western-Blot-NBP2-59051-img0006.jpg "Western Blot: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Analysis in human cell line RT-4.")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Staining of human duodenum shows strong cytoplasmic immunoreactivity in glandular cells and in lymphoid cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4463-Immunohistochemistry-Paraffin-NBP2-59051-img0005.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Staining of human prostate shows strong granular cytoplasmic immunoreactivity in glandular cells and moderate positivity in smooth muscle.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4463-Immunohistochemistry-Paraffin-NBP2-59051-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of cells as well as moderate immunoreactivity in neuropil.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4463-Immunohistochemistry-Paraffin-NBP2-59051-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Staining of human fallopian tube shows strong granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4463-Immunohistochemistry-Paraffin-NBP2-59051-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4463) [NBP2-59051] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous tubules.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4463-Immunohistochemistry-Paraffin-NBP2-59051-img0004.jpg)