Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0010.jpg "Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.")
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-NB600-607-img0011.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![TOR/mTOR [p Ser2448] Antibody [NB600-607]](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![ELISA: TOR/mTOR [p Ser2448] Antibody [NB600-607] - ELISA Results of TOR/TOR/mTOR [p Ser2448] antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-ELISA-NB600-607-img0012.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - TOR/mTOR (phospho Ser2448) antibody detects TOR/mTOR (phospho Ser2448) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right). Sample: Paraffin-embedded human lung cancer. TOR/mTOR (phospho Ser2448) stained by TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NBP3-13144-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0002.jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-2622024152248.jpg "Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: TOR/mTOR [p Ser2448] Antibody at 0.825ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241140938.jpg)
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Staining of Hela cells with TOR/mTOR [p Ser2448] Antibody (1G6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241312233.jpg)